Possibility of Bean yellow mosaic virus detection in Gladiolus plants by different methods

2011 ◽  
Vol 118 (1) ◽  
pp. 2-6 ◽  
Author(s):  
Ganesh S. Duraisamy ◽  
Radovan Pokorný ◽  
Ludmila Holková
Keyword(s):  
Mosaic Virus ◽  
Virus Detection ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus

scholarly journals The Complete Nucleotide Sequence of Bean Yellow Mosaic Virus Genomic RNA.

1996 ◽  
Vol 62 (5) ◽  
pp. 472-477 ◽  
Author(s):  
Shigeo NAKAMURA ◽  
Ryoso HONKURA ◽  
Takayoshi IWAI ◽  
Masashi UGAKI ◽  
Yuko OHASHI
Keyword(s):  
Nucleotide Sequence ◽  
Mosaic Virus ◽  
Complete Nucleotide Sequence ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Genomic Rna

scholarly journals PCNA Interacts with Indian Mung Bean Yellow Mosaic Virus Rep and Downregulates Rep Activity

2004 ◽  
Vol 78 (21) ◽  
pp. 11890-11903 ◽  
Author(s):  
Basavaraj Bagewadi ◽  
Shoajiang Chen ◽  
Sunil K. Lal ◽  
Nirupam Roy Choudhury ◽  
Sunil K. Mukherjee
Keyword(s):  
Mosaic Virus ◽  
Mung Bean ◽  
Bean Yellow Mosaic Virus ◽  
Yellow Mosaic Virus ◽  
Nuclear Antigen ◽  
Resting Cells ◽  
Rolling Circle Replication ◽  
Proliferative Cell Nuclear Antigen ◽  
Bipartite Genome

ABSTRACT Proliferative cell nuclear antigen (PCNA), a conserved plant protein as well as an important replication factor, is induced in response to geminivirus infection in the resting cells of the phloem tissues. The biochemical role of PCNA in rolling circle replication (RCR) of geminivirus DNA has not been explored in detail. The initiation of RCR of the bipartite genome of a geminivirus, Indian mung bean yellow mosaic virus (IMYMV), is mainly controlled by viral protein Rep (or AL1 or AC1). The role of host PCNA in RCR of IMYMV was revealed by studying the physical and functional interactions between recombinant PCNA and recombinant IMYMV Rep. Pea nuclear PCNA as well as recombinant pea PCNA showed binding to recombinant Rep in experiments involving both affinity chromatography and yeast two-hybrid approaches. The contacting amino acid residues of PCNA seemed to be present throughout a wide region of the trimeric protein, while those of Rep appeared to be localized only in the middle part of the protein. The site-specific nicking-closing activity and the ATPase function of IMYMV Rep were impaired by PCNA. These observations lead to interesting speculations about the control of viral RCR and dynamic profiles of protein-protein interactions at the RCR origin of the geminiviruses.

Sign in / Sign up

Export Citation Format

Share Document