Detection and molecular characterization of an aster yellows phytoplasma in rhodiola in Alberta, Canada

2009 ◽  
Vol 116 (4) ◽  
pp. 145-148
Author(s):  
S.-F. Hwang ◽  
J. Feng ◽  
R. Hwang ◽  
S. E. Strelkov ◽  
K. Ampong-Nyarko ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Aster Yellows ◽  
Aster Yellows Phytoplasma

Detection and molecular characterization of an aster yellows phytoplasma in parsley

1998 ◽  
Vol 20 (1) ◽  
pp. 55-61 ◽  
Author(s):  
A.-H. Khadhair ◽  
L. M. Kawchuk ◽  
R.C. Taillon ◽  
G. Botar
Keyword(s):  
Molecular Characterization ◽  
Aster Yellows ◽  
Aster Yellows Phytoplasma

scholarly journals Detection and molecular characterization of an aster yellows phytoplasma in poker statice and Queen Anne's lace in Alberta, Canada

2004 ◽  
Vol 159 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Kan-Fa Chang ◽  
Sheau-Fang Hwang ◽  
Abdul-Hameed Khadhair ◽  
Lawrence Kawchuk ◽  
Ronald Howard
Keyword(s):  
Molecular Characterization ◽  
Aster Yellows ◽  
Aster Yellows Phytoplasma

Molecular Characterization of Aster Yellows Subgroup 16SrI-B Phytoplasma in Verbena  × hybrida

2014 ◽  
Vol 163 (7-8) ◽  
pp. 664-669
Author(s):  
Jaroslava Přibylová ◽  
Karel Petrzik ◽  
Jana Fránová ◽  
Josef Špak
Keyword(s):  
Molecular Characterization ◽  
Aster Yellows

scholarly journals Detection and molecular characterization of phytoplasmas infecting apple trees in Poland

2014 ◽  
Vol 41 (No. 1) ◽  
pp. 27-33 ◽  
Author(s):  
M. Cieślińska ◽  
D.E. Kruczyńska
Keyword(s):  
Rflp Analysis ◽  
Rrna Gene ◽  
Apple Trees ◽  
Aster Yellows ◽  
Candidatus Phytoplasma Mali ◽  
Multiple Alignments ◽  
Pcr Rflp ◽  
Aster Yellows Phytoplasma ◽  
Reference Strains

During 2010&ndash;2012, samples from 225 apple trees growing in six regions of Poland were tested for phytoplasmas. 16S&nbsp;rRNA gene and 16S-23S spacer region sequences were amplified from total DNAs prepared from phloem tissue of apple shoots. According to the results of PCR-RFLP and sequence analyses, apple trees were infected by Candidatus Phytoplasma mali and Ca. P. asteris. Fragments of 16S rDNA plus 16S-23S spacer region of the Ca. P. mali isolates digested with HpaII enzyme showed two restriction profiles: P-I and P-II. Multiple alignments of 16S rRNA gene fragments revealed that the isolates of Ca. P. mali shared 100% sequence identity among themselves as well as with reference strains AT and AP-15 of apple proliferation phytoplasma. The nucleotide sequence of the same region of <br /> Ca. P. asteris isolates confirmed the phylogenetic relationship with reference strains OAY (MIAY) and AY1 of aster yellows phytoplasma PCR-RFLP analysis of ribosomal protein (rpl22 and rpS3), secY, and tuf genes did not show the sequence diversity of the isolates of aster yellows phytoplasma. &nbsp; &nbsp;

Characterization of putative membrane protein genes of the ‘Candidatus Phytoplasma asteris’, chrysanthemum yellows isolate

2008 ◽  
Vol 54 (5) ◽  
pp. 341-351 ◽  
Author(s):  
Luciana Galetto ◽  
Jacqueline Fletcher ◽  
Domenico Bosco ◽  
Massimo Turina ◽  
Astri Wayadande ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Expression Vector ◽  
Polyclonal Antibodies ◽  
Western Blots ◽  
Protein Fragments ◽  
Aster Yellows ◽  
Euscelidius Variegatus ◽  
Macrosteles Quadripunctulatus ◽  
Aster Yellows Phytoplasma

To characterize potentially important surface-exposed proteins of the phytoplasma causing chrysanthemum yellows (CY), new primers were designed based on the conserved regions of 3 membrane protein genes of the completely sequenced onion yellows and aster yellows witches’ broom phytoplasmas and were used to amplify CY DNA. The CY genes secY, amp, and artI, encoding the protein translocase subunit SecY, the antigenic membrane protein Amp and the arginine transporter ArtI, respectively, were cloned and completely sequenced. Alignment of CY-specific secY sequences with the corresponding genes of other phytoplasmas confirmed the 16S rDNA-based classification, while amp sequences were highly variable within the ‘Candidatus Phytoplasma asteris’. Five CY partial sequences were cloned into the pRSetC expression vector, and 3 of the encoded protein fragments (Amp 64/651, Amp 64/224, ArtI 131/512) were expressed as fusion antigens for the production of CY-specific polyclonal antibodies (A416 against Amp 64/224; A407 against ArtI 131/512). A416 recognized, in Western blots, the full-length Amp from CY-infected plants (periwinkle, daisy) and insect vectors ( Euscelidius variegatus , Macrosteles quadripunctulatus ). A416 also reacted to European aster yellows, to primula yellows phytoplasmas, to northern Italian strains of ‘Ca. Phytoplasma asteris’ from lettuce and gladiolus, but it did not react to American aster yellows phytoplasma.

Molecular characterization of a phytoplasma from the aster yellows (16SrI) group naturally infecting Populus nigra L. 'Italica' trees in Croatia

2003 ◽  
Vol 33 (2) ◽  
pp. 113-125 ◽  
Author(s):  
M. Seruga ◽  
D. Skoric ◽  
S. Botti ◽  
S. Paltrinieri ◽  
N. Juretic ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Populus Nigra ◽  
Aster Yellows
Sign in / Sign up

Export Citation Format

Share Document