Transfection with the cDNA of the human thyrotropin receptor of a poorly differentiated rat thyroid cell line (FRT)

1996 ◽  
Vol 19 (4) ◽  
pp. 230-235 ◽  
Author(s):  
R. Elisei ◽  
A. Pinchera ◽  
L. Chiovato ◽  
C. Mammoli ◽  
P. Agretti ◽  
...  
Keyword(s):  
Cell Line ◽  
Thyroid Cell ◽  
Thyrotropin Receptor ◽  
Thyroid Cell Line ◽  
Poorly Differentiated ◽  
Rat Thyroid

Properties and Regulation of the Thyrotropin Receptor in the FRTL5 Rat Thyroid Cell Line*

Endocrinology ◽  
1986 ◽  
Vol 118 (5) ◽  
pp. 1945-1951 ◽  
Author(s):  
DONATELLA TRAMONTANO ◽  
SIDNEY H. INGBAR
Keyword(s):  
Cell Line ◽  
Thyroid Cell ◽  
Thyrotropin Receptor ◽  
Thyroid Cell Line ◽  
Rat Thyroid

Characterization of the Subunit Structure of the Thyrotropin Receptor in the FRTL-5 Rat Thyroid Cell Line*

Endocrinology ◽  
1987 ◽  
Vol 121 (6) ◽  
pp. 2119-2130 ◽  
Author(s):  
SHARON E. GENNICK ◽  
COLIN G. THOMAS ◽  
SHIHADEH N. NAYFEH
Keyword(s):  
Cell Line ◽  
Thyroid Cell ◽  
Subunit Structure ◽  
Thyrotropin Receptor ◽  
Thyroid Cell Line ◽  
Rat Thyroid

Regulation of major histocompatibility complex class II antigen expression by the FRTL-5 rat thyroid cell line

1987 ◽  
Vol 115 (3) ◽  
pp. 481-487 ◽  
Author(s):  
A. P. Weetman ◽  
C. Green ◽  
L. K. Borysiewicz
Keyword(s):  
Major Histocompatibility Complex ◽  
Cell Line ◽  
Antigen Expression ◽  
Class Ii ◽  
Thyroid Cell ◽  
Histocompatibility Complex ◽  
Thyroid Cell Line ◽  
Rat Thyroid ◽  
Class Ii Antigen ◽  
Γ Interferon

ABSTRACT We have used the continuously growing FRTL-5 rat thyroid cell line to examine the regulation of major histocompatibility complex (MHC) class II (or la) antigen expression. Of the various stimuli investigated, only the supernatant from activated T cells or recombinant γ-interferon induced Ia expression. All Ia-inducing activity was removed from the T cell supernatant by acid dialysis, suggesting that γ-interferon is the single critical mediator for class II antigen expression. Its action was not TSH dependent but expression of class II antigens increased from the G0-G1 to the S and G2 phases of the cell cycle, so that TSH enhanced Ia expression by its action on cell division. Other agents including lectins, hormones, epidermal growth factor, a calcium ionophore and a phorbol ester did not induce Ia expression. Substances known to inhibit murine macrophage Ia expression (cortisol, prostaglandin E2 and 5-hydroxytryptamine) had no effect on FRTL-5 Ia expression. The use of this thyroid cell line has permitted direct examination of modulators in the absence of any possible effects from contaminating non-thyroid cells present in primary cultures and the results suggest that, of the agents tested, only γ-interferon has significance in the context of Ia antigen expression by the thyroid. J. Endocr. (1987) 115, 481–487

Adhesion molecule expression by the FRTL-5 rat thyroid cell line

1991 ◽  
Vol 130 (3) ◽  
pp. 451-456 ◽  
Author(s):  
N. Tandon ◽  
C. Dinsdale ◽  
T. Tamatani ◽  
M. Miyasaka ◽  
A. P. Weetman
Keyword(s):  
Cell Line ◽  
Adhesion Molecule ◽  
Interleukin 1 ◽  
Intercellular Adhesion Molecule ◽  
Thyroid Cell ◽  
Lymphocyte Function ◽  
Intercellular Adhesion Molecule 1 ◽  
Thyroid Cells ◽  
Thyroid Cell Line ◽  
Rat Thyroid

ABSTRACT We have examined the expression and function of rat CD54, a homologue of human intercellular adhesion molecule-1 (ICAM-1), by the continuously growing rat thyroid cell line FRTL-5. Approximately 10% of FRTL-5 cells express CD54 under basal conditions and this is not influenced by thyrotrophin. Expression of CD54 is increased by cytokines (γ-interferon, tumour necrosis factor, interleukin-1) and by an activator of C-kinase, phorbol 12-myristate 13-acetate. Blocking ICAM-1 with a monoclonal antibody directed against this molecule significantly (P <0·01) reduced the binding of splenic lymphocytes to FRTL-5 cells but inhibition was consistently greater (P <0·01) in the presence of antibodies against a rat homologue of lymphocyte function-associated antigen-1, the receptor on T cells for ICAM-1. In no case was complete blocking of cluster formation observed. These results show that a pure line of rat thyroid cells can express an ICAM-1 homologue and this is directly enhanced by cytokines. Expression of this homologue is partially responsible for lymphocyte adhesion to thyroid cells, which is likely to be a major event in T cell recognition of thyroid antigens in autoimmune thyroiditis. Journal of Endocrinology (1991) 130, 451–456

High-efficient Nonviral Transfection of the Rat Thyroid Cell Line FRTL-5

2010 ◽  
Vol 42 (12) ◽  
pp. 897-899 ◽  
Author(s):  
A. Klagge ◽  
K. Krause ◽  
K. Müller ◽  
J. Haag ◽  
D. Fuhrer
Keyword(s):  
Cell Line ◽  
Thyroid Cell ◽  
Thyroid Cell Line ◽  
High Efficient ◽  
Nonviral Transfection ◽  
Rat Thyroid
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