Ultrastructural morphology and evidence of iodide uptake and protein synthesis in normal human thyroid transplants in nude mice

1984 ◽  
Vol 7 (6) ◽  
pp. 569-578 ◽  
Author(s):  
S. Smeds ◽  
E. Jörtsö ◽  
B. Anderberg ◽  
B. Boeryd ◽  
L. E. Ericson ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Nude Mice ◽  
Human Thyroid ◽  
Iodide Uptake ◽  
Ultrastructural Morphology ◽  
Normal Human

IN-VITRO STUDIES OF NORMAL HUMAN THYROID CELLS: RESPONSES TO THYROTROPHIN AND DIBUTYRYL CYCLIC AMP

1977 ◽  
Vol 72 (1) ◽  
pp. 87-96 ◽  
Author(s):  
S. P. BIDEY ◽  
P. MARSDEN ◽  
J. ANDERSON ◽  
C. G. McKERRON ◽  
H. BERRY
Keyword(s):  
Cyclic Amp ◽  
Thyroid Tissue ◽  
Three Dimensional ◽  
Human Thyroid ◽  
Dibutyryl Cyclic Amp ◽  
Iodide Uptake ◽  
Thyroid Cells ◽  
Normal Human

SUMMARY Follicular cells isolated from normal human thyroid tissue have been cultured for up to 140 h with bovine thyrotrophin (TSH) or dibutyryl cyclic AMP (DBcAMP). Both compounds induced marked reorganization of the cells into three-dimensional follicular structures, whilst non-supplemented cells assumed a monolayer form. Cultures treated initially with TSH or DBcAMP showed a greater iodide uptake capacity, in comparison with unsupplemented cultures, in which iodide uptake was markedly diminished after 24 h. The release of tri-iodothyronine (T3) and thyroxine (T4) into the medium was determined by radioimmunoassay. Both TSH- and DBcAMP-treated cells showed a significant increase in iodothyronine output compared with unsupplemented control cells. In contrast to the 'classical' TSH-induced depression of the T4:T3 ratio in vivo, an increase in the ratio was observed for both TSH- and DBcAMP-supplemented cells in vitro. The ratio was also significantly greater after TSH than after DBcAMP, and possible implications of this finding are discussed.

scholarly journals Induction of follicle formation in long-term cultured normal human thyroid cells treated with thyrotropin stimulates iodide uptake but not sodium/iodide symporter messenger RNA and protein expression

2000 ◽  
Vol 167 (1) ◽  
pp. 125-135 ◽  
Author(s):  
T Kogai ◽  
F Curcio ◽  
S Hyman ◽  
EM Cornford ◽  
GA Brent ◽  
...  
Keyword(s):  
Messenger Rna ◽  
Sodium Iodide ◽  
Human Thyroid ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake ◽  
Thyroid Cells ◽  
Protein Levels ◽  
Normal Human ◽  
Tsh Stimulation ◽  
Stimulation Of

Iodide uptake by the sodium/iodide symporter (NIS) in thyrocytes is essential for thyroid hormone production. Reduced NIS activity has been reported in thyroid diseases, including thyroid cancer and congenital hypothyroidism. The study of iodide uptake in thyrocytes has been limited by the availability of appropriate in vitro models. A new culture technique was recently developed that allows normal human thyroid primary culture cells to grow as monolayer cells and express differentiated functions for more than 3 months. We used this technique to study the effect of follicle formation and TSH on iodide uptake in these cells. Iodide uptake by the cells grown in monolayer was very low. Follicle formation was induced from monolayer cells, and electron micrographs demonstrated cell polarity in the follicles. No significant increase in iodide uptake was observed after TSH treatment of cells in monolayer or when follicle formation was induced without TSH. TSH stimulation of follicles, however, significantly increased iodide uptake ( approximately 4. 4-fold; P<0.001). Compared with iodide uptake in monolayers, the combination of follicle formation and TSH treatment stimulated iodide uptake synergistically to 12.0-fold (P<0.001). NIS messenger RNA (mRNA) and protein levels were almost the same in both monolayer cells and follicles. TSH treatment of monolayers and follicles produced significant (P<0.05) stimulation of mRNA ( approximately 4. 8- and approximately 4.3-fold respectively) and protein ( approximately 6.8- and 4.9-fold respectively). TSH stimulated NIS protein levels in both monolayer and follicles, however, stimulation of functional iodide uptake was only seen with TSH stimulation of follicles. The function of NIS may involve post-transcriptional events, such as intracellular sorting, membrane localization of NIS or another NIS regulatory factor. Polarized functions, such as iodide efflux into follicular lumina, may also contribute to the increased iodide concentration after follicle formation.

Vascularization of Normal Human Thyroid Tissue Transplanted to Nude Mice

Pathobiology ◽  
1987 ◽  
Vol 55 (2) ◽  
pp. 104-114
Author(s):  
Johan M&ouml;lne ◽  
Erik J&ouml;rts&ouml; ◽  
Staffan Smeds ◽  
Lars E. Ericson
Keyword(s):  
Nude Mice ◽  
Thyroid Tissue ◽  
Human Thyroid ◽  
Human Thyroid Tissue ◽  
Normal Human

Ultrastructural modification of cellular interactions in cancer tumor

1978 ◽  
Vol 36 (2) ◽  
pp. 318-319
Author(s):  
N. P. Dmitrieva
Keyword(s):  
Cancer Cells ◽  
Human Thyroid ◽  
Adjacent Cell ◽  
Main Role ◽  
Cellular Interactions ◽  
Electron Microscopic ◽  
Cancer Tumor ◽  
Normal Human ◽  
Characteristic Features

One of the most characteristic features of cancer cells is their ability to metastasia. It is suggested that the modifications of the structure and properties of cancer cells surfaces play the main role in this process. The present work was aimed at finding out what ultrastructural features apear in tumor in vivo which removal of individual cancer cells from the cell population can provide. For this purpose the cellular interactions in the normal human thyroid and cancer tumor of this gland electron microscopic were studied. The tissues were fixed in osmium tetroxide and were embedded in Araldite-Epon.In normal human thyroid the most common type of intercellular contacts was represented by simple junction formed by the parallelalignment of adjacent cell membranees leaving in between an intermembranes space 15-20 nm filled with electronlucid material (Fig. 1a). Sometimes in the basal part of cells dilatations of the intercellular space 40-50 nm wide were found (Fig. 1a). Here the cell surfaces may form single short microvilli.

COMPOSITION EN ACIDES AMINES DE THYROGLOBULINES PROVENANT DE SUJETS EUTHYROIDIENS ET GOITREUX

1966 ◽  
Vol 53 (2) ◽  
pp. 297-302 ◽  
Author(s):  
Janine Bismuth ◽  
Marcel Rolland ◽  
Serge Lissitzky
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
Acid Composition ◽  
Human Thyroid ◽  
Thyroid Glands ◽  
Acides Aminés ◽  
Normal Human

ABSTRACT Amino acid composition of S19 thyroglobulin purified from three normal human thyroid glands, a hyperthyroid goiter, two hypothyroid familial goiters and a euthyroid goiter, has been determined. No significant differences in amino acid composition has been observed.

Parafollicular Cells in the Normal Human Thyroid

Nature ◽  
1971 ◽  
Vol 230 (5292) ◽  
pp. 334-335 ◽  
Author(s):  
STEVEN L. TEITELBAUM ◽  
KENNETH E. MOORE ◽  
WILLIAM SHIEBER
Keyword(s):  
Human Thyroid ◽  
Parafollicular Cells ◽  
Normal Human
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