Neurotransmitter regulation of somatostatin secretion by fetal rat cerebral cortical cells in culture

1993 ◽  
Vol 16 (9) ◽  
pp. 661-668 ◽  
Author(s):  
M. T. de los Frailes ◽  
L. Cacicedo ◽  
M. J. Lorenzo ◽  
R. M. Tolón ◽  
G. Fernández ◽  
...  
Keyword(s):  
Cortical Cells ◽  
Cells In Culture ◽  
Somatostatin Secretion ◽  
Fetal Rat

scholarly journals Transforming Growth Factor-βs Inhibit Somatostatin Messenger Ribonucleic Acid Levels and Somatostatin Secretion in Hypothalamic Cells in Culture*

Endocrinology ◽  
1997 ◽  
Vol 138 (10) ◽  
pp. 4401-4409 ◽  
Author(s):  
M. Quintela ◽  
R. M. SeñarÍs ◽  
C. Diéguez
Keyword(s):  
Growth Factor ◽  
Messenger Rna ◽  
Transforming Growth Factor ◽  
Inhibitory Effect ◽  
Mrna Levels ◽  
Messenger Ribonucleic Acid ◽  
Cells In Culture ◽  
Somatostatin Secretion ◽  
Hypothalamic Cells ◽  
The Stability

Abstract Treatment of hypothalamic cells in monolayer culture with transforming growth factor-β1 (TGFβ1) significantly reduced both basal and cAMP-induced somatostatin messenger RNA (mRNA) levels and somatostatin secretion. This inhibitory effect was dose- and time-dependent and not mediated by glial cells, as it was also observed in glial-free hypothalamic cell cultures treated with cytosine arabinonucleoside. TGFβ2 and -β3 mimicked the actions of TGFβ1, which indicated that the three isoforms of the TGFβ family expressed in the central nervous system displayed similar effects on the somatostatinergic neurons. The blockade of synthesis of proteins with either cycloheximide or puromycin for 24 h prevented the inhibitory effect of TGFβ1 on somatostatin mRNA. This implied that the reduction of this mRNA by TGFβ1 required de novo protein synthesis. We next studied whether TGFβ1 acted at the transcriptional or posttranscriptional level by altering the stability of somatostatin mRNA. Examination of the rate of disappearance of somatostatin mRNA by Northern blot, after inhibition of mRNA transcription with either actinomycin D (AcD) or 5,6-dichloro-1β-ribofuranosyl benzimidazole revealed that TGFβ1 did reduce the stability of somatostatin mRNA. This effect was observed when we pretreated the cultures with TGFβ1 4 h before the addition of AcD, but not when we administered TGFβ1 simultaneously with AcD or 5,6-dichloro-1β-ribofuranosyl benzimidazole. Altogether these results demonstrated that the treatment of hypothalamic cells in culture with TGFβ1, TGFβ2, or TGFβ3 resulted in a decrease in somatostatin mRNA levels and somatostatin secretion. TGFβ1 reduced the steady state levels of somatostatin mRNA by inducing the synthesis of a protein (s), that appears to accelerate the degradation of the mRNA of somatostatin. Whether TGFβ1 has additional effects on the transcription of the somatostatin gene will require further study.

Stimulatory effects of phenytoin on osteoblastic differentiation of fetal rat calvaria cells in culture

Bone ◽  
1999 ◽  
Vol 25 (6) ◽  
pp. 653-660 ◽  
Author(s):  
D Ikedo ◽  
K Ohishi ◽  
N Yamauchi ◽  
M Kataoka ◽  
J Kido ◽  
...  
Keyword(s):  
Osteoblastic Differentiation ◽  
Cells In Culture ◽  
Rat Calvaria ◽  
Fetal Rat

scholarly journals Effects of 5-bromodeoxyuridine on the ACTH-dependent mitochondrial biogenesis in cortical cells of fetal rat adrenals in tissue culture.

1976 ◽  
Vol 71 (3) ◽  
pp. 951-956 ◽  
Author(s):  
A I Kahri ◽  
M Salmenperä ◽  
A Saure
Keyword(s):  
Tissue Culture ◽  
Mitochondrial Dna ◽  
Dna Synthesis ◽  
Nuclear Dna ◽  
Acth Stimulation ◽  
Cortical Cells ◽  
Proliferative Phase ◽  
Fetal Rat ◽  
Rat Adrenals ◽  
Mitochondrial Dna Synthesis

Cortical cells of fetal rat adrenals in tissue culture were treated with 5-bromodeoxyuridine (BrdU) during their proliferative phase and during ACTH stimulation when nuclear DNA synthesis has almost ceased. Pretreatment with 0.5 mug/ml/day of BrdU inhibited the ACTH-induced differentiation of cortical cells as well as the secretion of corticosterone and 18-OH-deoxycorticosterone (18-OHDOC). When nuclear DNA synthesis was suppressed and mitochondrial DNA synthesis was stimulated by ACTH BrdU addition (30 mug/ml/day) permitted normal untrastructural differentiation of cortical cells, except that the development of mitochondrial inner membranes was inhibited. Simultaneously mitochondrial inner membranes was inhibited. Simultaneously mitochondrial 11beta- and 18-hydroxylations were strongly inhibited while cytoplasmic 21-hydroxylation was not affected.

scholarly journals Effects of 3-isobutyryl-2-isopropylpyrazolo-(1,5-a)-pyridine(Ibudilast) on hypoxic injury in fetal rat cortical cells.

Nosotchu ◽  
1994 ◽  
Vol 16 (5) ◽  
pp. 354-359
Author(s):  
Takayuki Sakaki ◽  
Kazuo Yamada ◽  
Hideo Otsuki ◽  
Takamichi Yuguchi ◽  
Toru Hayakawa
Keyword(s):  
Cortical Cells ◽  
Hypoxic Injury ◽  
Fetal Rat

Expression and characterization of parathyroid hormone receptors on fetal rat calvaria cells in culture

1994 ◽  
Vol 12 (S1) ◽  
pp. S33-S37 ◽  
Author(s):  
Noriyuki Yamauchi ◽  
Keiji Ohishi ◽  
Seiji Nishikawa ◽  
Toshihiko Nagata ◽  
Masatoshi Kataoka ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Hormone Receptors ◽  
Cells In Culture ◽  
Rat Calvaria ◽  
Fetal Rat

Thyroid Hormone Action on Biosynthesis of Somatostatin by Fetal Rat Brain Cells in Culture*

Endocrinology ◽  
1988 ◽  
Vol 123 (2) ◽  
pp. 898-904 ◽  
Author(s):  
MARIA TERESA DE LOS FRAILES ◽  
LUCINDA CACICEDO ◽  
MARIA JESUS LORENZO ◽  
GUMERSINDO FERNANDEZ ◽  
FRANCO SANCHEZ-FRANCO
Keyword(s):  
Thyroid Hormone ◽  
Rat Brain ◽  
Brain Cells ◽  
Hormone Action ◽  
Cells In Culture ◽  
Thyroid Hormone Action ◽  
Fetal Rat ◽  
Fetal Rat Brain
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