Expression of adhesion molecule ICAM-1 (CD54) in thyroid papillary adenocarcinoma

1994 ◽  
Vol 17 (11) ◽  
pp. 843-848 ◽  
Author(s):  
M. Nakashima ◽  
K. Eguchi ◽  
N. Ishikawa ◽  
I. Yamashita ◽  
M. Sakai ◽  
...  
Keyword(s):  
Adhesion Molecule ◽  
Papillary Adenocarcinoma ◽  
Thyroid Papillary ◽  
Thyroid Papillary Adenocarcinoma

scholarly journals Thyroid Papillary Adenocarcinoma in a Woman with Peutz-Jeghers Syndrome.

1992 ◽  
Vol 31 (9) ◽  
pp. 1117-1119 ◽  
Author(s):  
Masahiro YAMAMOTO ◽  
Hiroshi HOSHINO ◽  
Toshio ONIZUKA ◽  
Masafumi ICHIKAWA ◽  
Akitoshi KAWAKUBO ◽  
...  
Keyword(s):  
Papillary Adenocarcinoma ◽  
Thyroid Papillary ◽  
Peutz Jeghers Syndrome ◽  
Thyroid Papillary Adenocarcinoma

scholarly journals Hyperthyroidism in a Patient with TSH-Producing Pituitary Adenoma Coexisting with Thyroid Papillary Adenocarcinoma.

2000 ◽  
Vol 47 (6) ◽  
pp. 731-738 ◽  
Author(s):  
MASAYUKI KISHIDA ◽  
FUMIO OTSUKA ◽  
HIDEO KATAOKA ◽  
KAZUAKI YOKOTA ◽  
TETSUYA OISHI ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Papillary Adenocarcinoma ◽  
Thyroid Papillary ◽  
Thyroid Papillary Adenocarcinoma

Cavernous sinus metastasis from thyroid papillary adenocarcinoma

2002 ◽  
Vol 9 (5) ◽  
pp. 598-600 ◽  
Author(s):  
Toshihiro Takami ◽  
Kenji Ohata ◽  
Naohiro Tsuyuguchi ◽  
Ying Mao ◽  
Yuichi Inoue ◽  
...  
Keyword(s):  
Cavernous Sinus ◽  
Papillary Adenocarcinoma ◽  
Thyroid Papillary ◽  
Thyroid Papillary Adenocarcinoma

A pre-embedding double staining procedure used to observe the effect of fixation on the activity of intercellular adhesion molecule on T and B cells

1990 ◽  
Vol 48 (3) ◽  
pp. 378-379
Author(s):  
Jane E. Ramberg ◽  
Shigeto Tohma ◽  
Peter E. Lipsky
Keyword(s):  
B Cells ◽  
Adhesion Molecule ◽  
Colloidal Gold ◽  
Intercellular Adhesion ◽  
Intercellular Adhesion Molecule ◽  
Staining Procedure ◽  
Double Staining ◽  
T And B Cells ◽  
Microtiter Plates ◽  
Streptavidin Peroxidase

Intercellular adhesion molecule (ICAM-1) appears to be a ligand for LFA-1 dependent adhesion in T cell mediated cytotoxcity. It is found on cells of both hematopoietic and non-hematopoietic origin. While observing the activity of ICAM-1 on the surfaces of interacting T and B cells, we found that we could successfully carry out a pre-embedding double staining procedure utilizing both colloidal gold and peroxidase conjugated reagents.On 24-well microtiter plates, mitomycin-treated T4 cells were stimulated with 64.1 (anti-CD3) for one hour before the addition, in some instances, of B cells. Following a 12-48 hour incubation at 38°C, the cells were washed and then immunostained with a colloidal gold conjugated RFB-4 (anti-CD22); biotinylated R6.5 (anti-ICAM-1); followed by streptavidin/peroxidase. This method allowed us to observe two different antigens without concern about possible cross-reaction of reagents. Because we suspected ICAM-1 and R6.5 were sensitive to fixation, we tried varying concentrations of fresh paraformaldehyde before R6.5, after R6.5 and after streptavidin/peroxidase. All immunostaining and washing was done on ice with ice cold reagents.

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