Agrobacterium tumefaciens-Mediated Genetic Transformation of Sugar Beet (Beta vulgaris L.)

1994 ◽  
Vol 3 (1) ◽  
pp. 37-41 ◽  
Author(s):  
B. K. Konwar
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Sugar Beet ◽  
Genetic Transformation ◽  
Beta Vulgaris

Transformation and regeneration in sugar beet (Beta vulgaris L.) induced by ?shooter? mutants of Agrobacterium tumefaciens

Euphytica ◽  
1988 ◽  
Vol 39 (S3) ◽  
Author(s):  
F.A. Krens ◽  
C. Zijlstra ◽  
W.v.d. Molen ◽  
D. Jamar ◽  
H.J. Huizing
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Shooter Mutants

scholarly journals Creation of transgenic sugar beet lines containing synthetic gene cry1C

1970 ◽  
pp. 221-225
Author(s):  
V. V. Kurylo ◽  
E. N. Shysha ◽  
A. I. Yemets
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Sugar Beet ◽  
Genetic Transformation ◽  
Marker Gene ◽  
Leaf Explants ◽  
Plant Genome ◽  
Direct Regeneration ◽  
Pcr Analysis ◽  
Cry Genes ◽  
Sugar Beet Crop

Aim. Insect pest’s impact makes a significant limitation of the sugar beet crop yield. Integration of cry-genes of Bacillus thuringiensis into the plant genome is one of the promising strategies to ensure of plant resistance. The aim of this work was the production of sugar beet lines (based on the MM1/2 line) expressing cry1C genes. Methods. Genetic transformation of sugar beet was performed using the method of co-cultivation of leaf explants with Agrobacterium tumefaciens. Results. Sugar beet line MM1/2 was transformed by Agrobacterium-mediated method of transformation using binary vector pRD400-cry1CST, containing synthetic cry1C gene and selectable marker gene neomycin phosphotransferase II (nptII) conferring resistance to kanamycin. After the optimization protocol of genetic transformation and direct regeneration from leaf discs a transgenic sugarbeet lines were obtained. Conclusions. PCR analysis revealed integration of cry1C into the genome of transgenic lines of B. vulgaris.Keywords: genetic transformation, Agrobacterium tumefaciens, Beta vulgaris, cry-genes.

Susceptibility of sugar beet (Beta vulgaris L.) genotypes at different ploidy levels to Agrobacterium tumefaciens infection

2012 ◽  
Vol 29 ◽  
pp. S134
Author(s):  
Cansu Telci Kahramanoğullari ◽  
Selcen Darcin ◽  
Ayse Simsek ◽  
Burak Onol ◽  
Mustafa Yildiz
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Ploidy Levels

Plant regeneration from protoplasts of sugar beet (Beta vulgaris)

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder
Keyword(s):  
Plant Regeneration ◽  
Sugar Beet ◽  
Beta Vulgaris

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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