Host Range of an Insecticidal Crystal Protein of Bacillus thuringiensis subsp. kurstaki Produced in Escherichia coli

1994 ◽  
Vol 3 (1) ◽  
pp. 15-18 ◽  
Author(s):  
P. V. A. L. Ratnakar ◽  
N. Vijayalakshmi ◽  
Jyoti Kapila ◽  
H. D. Rananavare ◽  
Manjula Mathur ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Bacillus Thuringiensis ◽  
Host Range ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein ◽  
Bacillus Thuringiensis Subsp

Characterization and expression of a novel holotype insecticidal crystal protein gene from nativeBacillus thuringiensisBM59-2

2010 ◽  
Vol 56 (2) ◽  
pp. 156-161 ◽  
Author(s):  
Aiping Zheng ◽  
Jun Zhu ◽  
Lingxia Wang ◽  
Shuangcheng Li ◽  
Qiming Deng ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Sequence Analysis ◽  
Bacillus Thuringiensis ◽  
Aedes Aegypti ◽  
Larvicidal Activity ◽  
Insecticidal Activity ◽  
Protein Gene ◽  
Crystal Protein ◽  
Insecticidal Crystal Protein ◽  
Escherichia Coli Bl21

We characterized a novel holotype cry gene (cry52Ba1) harbored in a Bacillus thuringiensis isolate BM59-2 native to China that expresses a spherical crystal protein of about 80 kDa. In this study, the full length of the cry52Ba1 gene was cloned from this strain. Sequence analysis of the gene was also performed. Furthermore, cry52Ba1 was expressed in Escherichia coli BL21(DE)pLysS, and the resulting insecticidal activity showed that the Cry52Ba1 protein exhibited high larvicidal activity against Aedes aegypti (Diptera), with a lethal concentration 50 of 1.526 µg/mL (95% confidence: 0.740–3.499 µg/mL).

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