Identification and Partial Characterization of Trypsin Inhibitory Activity in Seed of Some Fruit Plants

2010 ◽  
Vol 19 (2) ◽  
pp. 235-237 ◽  
Author(s):  
Deepankar Gahloth ◽  
Ashwani Kumar Sharma
Keyword(s):  
Inhibitory Activity ◽  
Partial Characterization ◽  
Trypsin Inhibitory Activity ◽  
Fruit Plants

scholarly journals Inhibition of protohaem ferro-lyase in experimental porphyria. Isolation and partial characterization of a modified porphyrin inhibitor

1980 ◽  
Vol 188 (1) ◽  
pp. 145-152 ◽  
Author(s):  
F De Matteis ◽  
A H Gibbs ◽  
T R Tephly
Keyword(s):  
Inhibitory Activity ◽  
Partial Characterization ◽  
Selective Reagent ◽  
Radioactive Drug ◽  
Active Centres

1. A modified porphyrin with inhibitory activity towards protohaem ferro-lyase was purified from the livers of mice treated with 3,5-diethoxycarbonyl-1,4-dihydrocollidine, and partially characterized. 2. The inhibitor can be labelled by 5-amino[4-14C]-laevulinate, suggesting that it originates from pre-labelled liver haem. No radioactivity from 3,5-diethoxycarbonyl-1,4-dihydro[2,6-14C]collidine can be recovered bound to the purified abnormal porphyrin when the radioactive drug is used to induce its formation. 3. Similar modified porphyrins isolated from the livers of animals treated with 2-allyl-2-isopropylacetamide, secobarbitone or 1-ethynylcyclohexanol did not exhibit inhibitory activity toward protohaem ferro-lyase. 4. The inhibition of protohaem ferro-lyase was progressive, could be slowed down by cooling and partially prevented by preincubating the enzyme with the porphyrin substrate. Once established, inhibition could not be reversed by addition of the substrate 5. These results suggest that the modified porphyrin irreversibly inhibits protohaem ferro-lyase and may be used as a sensitive and selective reagent to titrate the number of active centres of the enzyme.

Characterization of a Saccharide-Binding Protein from Talisia esculenta Seeds with Trypsin Inhibitory Activity

2009 ◽  
Vol 16 (12) ◽  
pp. 1557-1564 ◽  
Author(s):  
Maria das Gracas Freire ◽  
Ilka Vasconcelos ◽  
Marcos Oliveira ◽  
Goncalo de Souza Filho ◽  
Maria Ligia Macedo
Keyword(s):  
Inhibitory Activity ◽  
Binding Protein ◽  
Saccharide Binding ◽  
Trypsin Inhibitory Activity

Characterization of an Abnormal Antithrombin (Milano 2) with Defective Thrombin Binding

1986 ◽  
Vol 56 (03) ◽  
pp. 349-352 ◽  
Author(s):  
A Tripodi ◽  
A Krachmalnicoff ◽  
P M Mannucci
Keyword(s):  
Venous Thromboembolism ◽  
Active Site ◽  
Inhibitory Activity ◽  
Antithrombin Iii ◽  
Factor Xa ◽  
Molecular Defect ◽  
Affinity Adsorption ◽  
Italian Family ◽  
Crossed Immunoelectrophoresis

SummaryFour members of an Italian family (two with histories of venous thromboembolism) had a qualitative defect of antithrombin III reflected by normal antigen concentrations and halfnormal antithrombin activity with or without heparin. Anti-factor Xa activities were consistently borderline low (about 70% of normal). For the propositus’ plasma and serum the patterns of antithrombin III in crossed-immunoelectrophoresis with or without heparin were indistinguishable from those of normal plasma or serum. A normal affinity of antithrombin III for heparin was documented by heparin-sepharose chromatography. Affinity adsorption of the propositus’ plasma to human α-thrombin immobilized on sepharose beads revealed defective binding of the anti thrombin III to thrombin-sepharose. Hence the molecular defect of this variant appears to be at the active site responsible for binding and neutralizing thrombin, thus accounting for the low thrombin inhibitory activity.

Partial characterization of the N-linked oligosaccharides occurring on rat hepatocyte glycoproteins

2010 ◽  
Vol 108 (10) ◽  
pp. 323-329 ◽  
Author(s):  
Marti F. A. Bierhuizen ◽  
Moniek de Wit ◽  
Carin A. R. L. Govers ◽  
Willem van Dijk
Keyword(s):  
Partial Characterization ◽  
Rat Hepatocyte

scholarly journals Localization and Partial Characterization of an Aldehydic Component in Tropocollagen

1966 ◽  
Vol 241 (7) ◽  
pp. 1530-1536
Author(s):  
Marcos Rojkind ◽  
Olga O. Blumenfeld ◽  
Paul M. Gallop
Keyword(s):  
Partial Characterization
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