Synthesis of Silver Nanoparticles Using Callus Extract of Carica papaya — A First Report

2008 ◽  
Vol 18 (1) ◽  
pp. 83-86 ◽  
Author(s):  
Namrata Mude ◽  
Avinash Ingle ◽  
Aniket Gade ◽  
Mahendra Rai
Keyword(s):  
Silver Nanoparticles ◽  
Carica Papaya ◽  
First Report

scholarly journals Antimicrobial and Anticancer properties of Carica papaya leaves derived di-methyl flubendazole mediated silver nanoparticles

2021 ◽  
Author(s):  
Sandhanasamy Devanesan ◽  
Murugesan Jayamala ◽  
Mohamad S. AlSalhi ◽  
Amirtham S. Umamaheshwari ◽  
Jacob A. Ranjitsingh
Keyword(s):  
Silver Nanoparticles ◽  
Carica Papaya ◽  
Anticancer Properties

scholarly journals First Report of Anthracnose of Papaya (Carica papaya L.) Caused by Colletotrichum siamense in China

Plant Disease ◽  
2021 ◽  
Author(s):  
Yujie Zhang ◽  
Wenxiu Sun ◽  
Ping Ning ◽  
Tangxun Guo ◽  
SuiPing Huang ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Disease Incidence ◽  
Aerial Mycelium ◽  
Postharvest Disease ◽  
Distilled Water ◽  
First Report ◽  
Surface Samples ◽  
Initial Symptoms ◽  
Colletotrichum Siamense ◽  
Pathogenicity Tests

Papaya (Carica papaya L.) is a rosaceous plant widely grown in China, which is economically important. Anthracnose caused by Colletotrichum sp. is an important postharvest disease, which severely affects the quality of papaya fruits (Liu et al., 2019). During April 2020, some mature papaya fruits with typical anthracnose symptoms were observed in Fusui, Nanning, Guangxi, China with an average of 30% disease incidence (DI) and over 60% DI in some orchards. Initial symptoms of these papayas appeared as watery lesions, which turned dark brown, sunken, with a conidial mass appearing on the lesions under humid and warm conditions. The disease severity varied among fruits, with some showing tiny light brown spots, and some ripe fruits presenting brownish, rounded, necrotic and depressed lesions over part of their surface. Samples from two papaya plantations (107.54°E, 22.38°N) were collected, and brought to the laboratory. Symptomatic diseased tissues were cut into 5 × 5 mm pieces, surface sterilized with 2% (v/v) sodium hypochlorite for 1 minute, and rinsed three times with sterilized water. The pieces were then placed on potato dextrose agar (PDA). After incubation at 25°C in the dark for one week, colonies with uniform morphology were obtained. The aerial mycelium on PDA was white on top side, and concentric rings of salmon acervuli on the underside. A gelatinous layer of spores was observed on part of PDA plates after 7 days at 28°C. The conidia were elliptical, aseptate and hyaline (Zhang et al., 2020). The length and width of 60 conidia were measured for each of the two representative isolates, MG2-1 and MG3-1, and these averaged 13.10 × 5.11 μm and 14.45 × 5.95 μm. DNA was extracted from mycelia of these two isolates with the DNA secure Plant Kit (TIANGEN, Biotech, China). The internal transcribed spacer (ITS), partial actin (ACT), calmodulin (CAL), chitin synthase (CHS), β-tubulin 2 (TUB2) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) regions were amplified by PCR and sequenced. The sequences were deposited into GenBank with accessions MT904003, MT904004, and MT898650 to MT898659. BLASTN analyses against the GenBank database showed that they all had over 99% identity to the type strain of Colletotrichum siamense isolate ICMP 18642 (GenBank accession numbers JX010278, GQ856775, JX009709, GQ856730, JX010410, JX010019) (Weir et al., 2012). A phylogenetic tree based on the combined ITS, ACT, CAL, CHS, TUB2 and GAPDH sequences using the Neighbor-joining algorithm also showed that the isolates were C. siamense. Pathogenicity tests were conducted on 24 mature, healthy and surface-sterilized papaya fruits. On 12 papaya fruits, three well separated wounded sites were made for inoculation, and for each wounded site, six adjacent pinhole wounds were made in a 5-mm-diameter circular area using a sterilized needle. A 10 µl aliquot of 1 × 106 conidia/ml suspension of each of the isolates (MG2-1 and MG3-1) was inoculated into each wound. For each isolate, there were six replicate fruits. The control fruits were inoculated with sterile distilled water. The same inoculation was applied to 12 non-wound papaya fruits. Fruits were then placed in boxes which were first washed with 75% alcohol and lined with autoclaved filter paper moistened with sterilized distilled water to maintain high humidity. The boxes were then sealed and incubated at 28°C. After 10 days, all the inoculated fruits showed symptoms, while the fruits that were mock inoculated were without symptoms. Koch's postulates were fulfilled by re-isolation of C. siamense from diseased fruits. To our knowledge, this is the first report of C. siamense causing anthracnose of papaya in China. This finding will enable better control of anthracnose disease caused by C. siamense on papaya.

scholarly journals First report of biomimetic synthesis of silver nanoparticles using aqueous callus extract of Centella asiatica and their antimicrobial activity

2014 ◽  
Vol 5 (7) ◽  
pp. 801-807 ◽  
Author(s):  
Vasudeva Reddy Netala ◽  
Venkata Subbaiah Kotakadi ◽  
Venkateswarlu Nagam ◽  
Pushpalatha Bobbu ◽  
Sukhendu Bikash Ghosh ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Centella Asiatica ◽  
Biomimetic Synthesis ◽  
First Report

scholarly journals Comparative Study on Antibacterial Activity of Crude and Silver Nanoparticles Synthesized Leaf Extract of Carica papaya against Chosen Isolates

2021 ◽  
Vol 70 (1) ◽  
Author(s):  
Pandiarajan. P ◽  
Andrew Pradeep ◽  
Januja J ◽  
Dhurairaj Satheesh
Keyword(s):  
Silver Nanoparticles ◽  
Antibacterial Activity ◽  
Carica Papaya ◽  
Proteolytic Enzymes ◽  
Antibiotic Resistance Genes ◽  
Human Pathogens ◽  
Zone Of Inhibition ◽  
Antimicrobial Drugs ◽  
Ft Ir ◽  
Phytochemical Components

The Emergence of Human Pathogens with Antibiotic resistance genes has shifted the focus towards the plant origin antimicrobial drugs with advanced nano-based methods. Screening of phytochemical components is done using various biochemical methods. UVvisible spectrophotometer is utilized to determine the silver Nanoparticle's size, shape, and its stable nature in the aqueous colloidal solution. FT-IR spectral analysis to identify the bio molecules responsible for the reduction of Ag+ ions. The extracts of Carica papaya leaves were found to be rich in Vitamins, Phenols, Proteolytic enzymes which acts as excellent Antimicrobial agent. Antibacterial activity of crude extracts and silver Nanoparticles synthesized from Carica papaya leaves was evaluated against chosen clinical isolates, which shows the effective zone of inhibition lesser side effects. Though crude extract has shown significant results, silver nanoparticles synthesized using specific key phytochemical component would have the better inhibiting capacity.

scholarly journals First Report of Papaya (Carica papaya) Anthracnose Caused by Colletotrichum plurivorum in Mexico

Plant Disease ◽  
2020 ◽  
Vol 104 (2) ◽  
pp. 589-589
Author(s):  
R. S. García-Estrada ◽  
I. Cruz-Lachica ◽  
L. A. Osuna-García ◽  
I. Márquez-Zequera
Keyword(s):  
Carica Papaya ◽  
First Report

scholarly journals A purely green synthesis of silver nanoparticles using Carica papaya, Manihot esculenta, and Morinda citrifolia: synthesis and antibacterial evaluations

2017 ◽  
Vol 40 (9) ◽  
pp. 1349-1361 ◽  
Author(s):  
Achmad Syafiuddin ◽  
Salmiati ◽  
Tony Hadibarata ◽  
Mohd Razman Salim ◽  
Ahmad Beng Hong Kueh ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Manihot Esculenta ◽  
Carica Papaya ◽  
Morinda Citrifolia
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