High Frequency Regeneration of Brassica napus Varieties and Genetic Transformation of Stocks Containing Fertility Restorer Genes for Two Cytoplasmic Male Sterility Systems

2000 ◽  
Vol 9 (2) ◽  
pp. 73-79 ◽  
Author(s):  
Sanjay Kumar Phogat ◽  
Pradeep Kumar Burma ◽  
Deepak Pental
Keyword(s):  
Brassica Napus ◽  
Genetic Transformation ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
High Frequency ◽  
Fertility Restorer ◽  
Restorer Genes ◽  
Fertility Restorer Genes

Male sterility in Indian mustard (Brassica juncea (L.) Coss). IV. Genetics of MS-4

1985 ◽  
Vol 27 (5) ◽  
pp. 487-490 ◽  
Author(s):  
Surinder S. Banga ◽  
K. S. Labana
Keyword(s):  
Male Sterility ◽  
Brassica Juncea ◽  
Indian Mustard ◽  
Field Trials ◽  
Genetic Constitution ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Male Sterile Plant ◽  
Restorer Genes ◽  
Fertility Restorer Genes

Male sterile plants of Indian mustard (Brassica juncea (L.) Coss.) were observed in the F2 generation of the cross RLM-198 × EJ-33. The genetic analysis revealed that male sterility occurred when the cytoplasm of RLM-198 interacted with recessive nuclear genes of EJ-33. The genetic constitution of RLM-198 was postulated to be (S) RF RF, EJ-33 as (F) rf rf, and the male sterile plants as (S) rf rf. Varieties of Indian mustard from India mostly contained dominant fertility restorer genes, while European varieties had a greater frequency of the recessive maintainer genes. None of these varieties, however, was capable of complete maintenance of male sterility. Heterosis for yield up to 56% over the national check was observed in field trials. The use of this cytoplasmic male sterile plant in hybrid mustard production will not be economical, until a complete maintainer for male sterility is identified.Key words: Brassica juncea, Indian mustard, male sterility, hybrids.

A binary vector-based large insert library for Brassica napus and identification of clones linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Yongzhong Wu ◽  
Lomas Tulsieram ◽  
Quangzhou Tao ◽  
Hong-Bin Zhang ◽  
Steven J Rothstein
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Plant Transformation ◽  
Transformation Efficiency ◽  
Binary Vector ◽  
Brassica Species ◽  
Chromosome Walking ◽  
Fertility Restorer ◽  
Plant Transformation Vector

We constructed and characterized a large DNA insert library for Brassica napus that would facilitate genome-related research and map-based cloning efforts in Brassica species. This library, consisting of 92 160 clones arrayed in 384-well microtiter dishes, was based on a conventional plant transformation vector (binary vector), and was constructed using a single ligation with transformation efficiency of over 5000 recombinants per microliter of ligation mixture. Every clone in this library contains an insert in the size range of 30-190 kb, facilitating both chromosome walking and plant transformation. Screening this library with three DNA markers (C2, F10, and CabR) that are linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS) identified at least 17 positive clones for each probe. Among the 17 positive clones identified by C2, nine are linked to the restorer locus. Marker F10 identified 21 clones, of which only two are linked to the restorer locus. None of 68 clones identified by CabR is linked to the restorer locus. A stability test using two clones identified by the C2 marker indicated that large DNA inserts are stable in this conventional vector in both Escherichia coli and Agrobacterium. Key words: Brassica napus, binary vector, large DNA insert library, restorer gene.

Identification and Inheritance of Fertility Restorer Genes for 'tour' CMS in Rapeseed (Brassica napus L.)

1994 ◽  
Vol 112 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Y. S. Sodhi ◽  
A. K. Pradhan ◽  
J. K. Verma ◽  
N. Arumugam ◽  
A. Mukhopadhyay ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Restorer Genes ◽  
Fertility Restorer Genes

Alloplasmic male sterile Brassica napus with Enarthrocarpus lyratus cytoplasm: introgression and molecular mapping of an E. lyratus chromosome segment carrying a fertility restoring gene

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 792-797 ◽  
Author(s):  
H S Janeja ◽  
S K Banga ◽  
P B Bhaskar ◽  
S S Banga
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Molecular Mapping ◽  
Bulked Segregant Analysis ◽  
Fertility Restoration ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Fertility Restorer ◽  
Donor Species

A cytoplasmic male sterility (CMS) system for Brassica napus (2n = 38; AACC) was developed by backcross substitution of its nucleus into the cytoplasm of a wild crucifer, Enarthrocarpus lyratus. Male sterility was complete, stable, and expressed in small flowers with rudimentary anthers. Since the B. napus germplasm lines were complete or partial maintainers of male sterility, the required fertility restorer gene (Rfl) was introgressed from the cytoplasm donor species. Inheritance studies carried out on F1 and F2 populations derived from hybridizing cytoplasmic male sterile and male fertile near-isogenic (PNILs) lines of B. napus 'Westar', revealed a monogenic dominant control for fertility restoration. Bulked segregant analysis with 215 RAPD primers helped in the identification of putative primers associated with fertility restoration. Co-segregation analysis of eight such primers with Rfl gene revealed two markers, OPK 15700 and OPZ 061300, which flank the Rfl locus on either side at a distance of 8.2 and 2.5 cM, respectively. These DNA markers will be useful in marker-assisted selection for improving the commercial potential of this newly developed CMS-fertility-restorer system for hybrid seed production programs in rapeseed.Key words: oilseed rape, hybrids, cytoplasmic male sterility, fertility restoration, RAPD mapping.

Inheritance of male fertility restoration and allelism of restorer genes for the Polima cytoplasmic male sterility system in oilseed rape

Genome ◽  
1989 ◽  
Vol 32 (6) ◽  
pp. 1044-1047 ◽  
Author(s):  
G. H. Fang ◽  
P. B. E. McVetty
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Restorer Gene ◽  
Fertility Restorer ◽  
Brassica Napus L ◽  
Restorer Genes ◽  
Segregation Ratios ◽  
Male Fertility Restoration

The inheritance of male fertility restoration for the pol cytoplasmic male sterility (CMS) system in summer rape (Brassica napus L.) was determined. Male fertility:sterility segregation ratios observed in F2 and backcross generations derived from crosses and backcrosses between two pol CMS A lines and the male fertility restorer gene(s) sources Italy and UM2353 were used in this study. Italy and UM2353 were found to possess a single Mendelian dominant gene with high male fertility restoration capabilities for the pol CMS system. Tests for allelism of the restorer genes were also conducted using male fertility:sterility segregation ratios observed in F3 families derived from crosses between F1 plants containing genes for male fertility restoration from the Italy and UM2353 restorer gene sources. The male fertility restorer gene from Italy (designated Rfp1) was found to be different (i.e., nonallelic) from the restorer gene possessed by UM2353 (designated Rfp2).Key words: Brassica napus L., oilseed rape, male fertility restoration, pol cytoplasmic male sterility.

Evaluation of the cost of restoration of male fertility in Brassica napus

Botany ◽  
2014 ◽  
Vol 92 (11) ◽  
pp. 847-853 ◽  
Author(s):  
Benjamin R. Montgomery ◽  
Maia F. Bailey ◽  
Gregory G. Brown ◽  
Lynda F. Delph
Keyword(s):  
Brassica Napus ◽  
Sex Determination ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Fertility Restoration ◽  
Brassica Napus L ◽  
Pollen Counts ◽  
Restorer Genes ◽  
The Cost

Gynodioecy frequently results from the interplay of mitochondrial cytoplasmic male sterility (CMS) and nuclear fertility-restoration genes. Models suggest that maintaining cytonuclear gynodioecy requires that restorer genes incur a cost to fitness because otherwise they would increase toward fixation. Direct tests of costs of restorer alleles require knowledge of the underlying genetics of sex determination. We use a well characterized CMS system in Brassica napus L. to measure aspects of fitness in four lineages that vary in whether they carry the pol CMS gene or male-fertile cytoplasm (cam), and whether they carry the Rfp restorer of pol or Rfn restorer of the nap CMS gene. As expected, plants with pol CMS and only the Rfn restorer experienced reduced flower size, stamen length, and pollen counts. Plants with pol and the Rfp restorer showed incomplete restoration with shorter stamens than both lines with cam cytoplasm and reduced pollen counts compared with plants with cam cytoplasm and the Rfp restorer. Among plants with cam cytoplasm, pollen counts were higher for those with the Rfp than Rfn restorer, indicating a greater cost of restoration associated with Rfn. These results demonstrate that costs of restoration differ for the Rfn and Rfp alleles in B. napus.

A binary vector-based large insert library for Brassica napus and identification of clones linked to a fertility restorer locus for Ogura cytoplasmic male sterility (CMS)

Genome ◽  
2000 ◽  
Vol 43 (1) ◽  
pp. 102-109 ◽  
Author(s):  
Yongzhong Wu ◽  
Lomas Tulsieram ◽  
Quangzhou Tao ◽  
Hong-Bin Zhang ◽  
Steven J. Rothstein
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Binary Vector ◽  
Fertility Restorer

Cytoplasmic male sterility in rapeseed (Brassica napus L.): female fertility of restored rapeseed with "Ogura" and cybrids cytoplasms

Genome ◽  
1988 ◽  
Vol 30 (2) ◽  
pp. 234-238 ◽  
Author(s):  
R. Pellan-Delourme ◽  
M. Renard
Keyword(s):  
Brassica Napus ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Male Fertility ◽  
Raphanus Sativus ◽  
Seed Set ◽  
Embryo Sac ◽  
Female Fertility ◽  
Brassica Napus L ◽  
Restorer Genes

The study of Brassica napus L. plants carrying restorer genes introgressed from radish (Raphanus sativus L.) showed that these genes ensured restoration of male fertility in rapeseed for all the male sterility-inducing cytoplasm studied, i.e., "Ogura"-type cytoplasm and that of four cybrids obtained by protoplast fusion. Plants with high levels of restored male fertility were obtained. However, the introduction of restorer genes was accompanied by a large decrease in seed set. Observations of embryo sacs inside the ovules and correlation between number of seeds per pod and percentage of octonucleate embryo sacs indicated that low seed set could be attributed to a high rate of embryo sac abortion, mainly at the uninucleate stage. Introduction of too much radish genetic information was assumed to be the cause of this low female fertility. Female fertility must be improved before the restored material can be used for F1 hybrid rapeseed production.Key words: Brassica napus, Raphanus sativus, cytoplasmic male sterility, restorer, cybrid, female fertility, embryo sac.

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