Expression of Multiple Virus-derived Resistance Determinants in Transgenic Plants Does Not Lead to Additive Resistance Properties

Indu B. Maiti; Carol Von Lanken; Yiling Hong; Nrisingha Dey; Arthur G. Hunt

doi:10.1007/bf03263061

Expression of Multiple Virus-derived Resistance Determinants in Transgenic Plants Does Not Lead to Additive Resistance Properties

Journal of Plant Biochemistry and Biotechnology ◽

10.1007/bf03263061 ◽

1999 ◽

Vol 8 (2) ◽

pp. 67-73 ◽

Cited By ~ 5

Author(s):

Indu B. Maiti ◽

Carol Von Lanken ◽

Yiling Hong ◽

Nrisingha Dey ◽

Arthur G. Hunt

Keyword(s):

Transgenic Plants ◽

Resistance Determinants ◽

Multiple Virus ◽

Additive Resistance

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A minimum length of N gene sequence in transgenic plants is required for RNA-mediated tospovirus resistance

Microbiology ◽

10.1099/0022-1317-81-1-235 ◽

2000 ◽

Vol 81 (1) ◽

pp. 235-242 ◽

Cited By ~ 40

Author(s):

Fuh-Jyh Jan ◽

Carmen Fagoaga ◽

Sheng-Zhi Pang ◽

Dennis Gonsalves

Keyword(s):

Transgenic Plants ◽

Gene Silencing ◽

Tobacco Mosaic Virus ◽

Virus Resistance ◽

N Gene ◽

Transcriptional Gene Silencing ◽

Viral Gene ◽

Minimum Length ◽

Post Transcriptional Gene Silencing ◽

Multiple Virus

We showed previously that transgenic plants with the green fluorescent protein (GFP) gene fused to segments of the nucleocapsid (N) gene of tomato spotted wilt virus (TSWV) displayed post-transcriptional gene silencing of the GFP and N gene segments and resistance to TSWV. These results suggested that a chimeric transgene composed of viral gene segments might confer multiple virus resistance in transgenic plants. To test this hypothesis and to determine the minimum length of the N gene that could trans-inactivate the challenging TSWV, transgenic plants were developed that contained GFP fused with N gene segments of 24–453 bp. Progeny from these plants were challenged with: (i) a chimeric tobacco mosaic virus containing the GFP gene, (ii) a chimeric tobacco mosaic virus with GFP plus the N gene of TSWV and (iii) TSWV. A number of transgenic plants expressing the transgene with GFP fused to N gene segments from 110 to 453 bp in size were resistant to these viruses. Resistant plants exhibited post-transcriptional gene silencing. In contrast, all transgenic lines with transgenes consisting of GFP fused to N gene segments of 24 or 59 bp were susceptible to TSWV, even though the transgene was post-transcriptionally silenced. Thus, virus resistance and post-transcriptional gene silencing were uncoupled when the N gene segment was 59 bp or less. These results provide evidence that multiple virus resistance is possible through the simple strategy of linking viral gene segments to a silencer DNA such as GFP.

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