Mitochondrial DNA-RFLP Analysis Distinguishes New CMS Sources in Pearl Millet (Pennisetum glaucum (L.) R. Br.)

1998 ◽  
Vol 7 (2) ◽  
pp. 85-92 ◽  
Author(s):  
A. K. Chhabra ◽  
K. N. Rai ◽  
I. S. Khairwal ◽  
S. Sivaramakrishnan ◽  
C. T. Hash
Keyword(s):  
Mitochondrial Dna ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Rflp Analysis

scholarly journals RFLP analysis of mitochondrial DNA from cytoplasmic male-sterile lines of pearl millet

1994 ◽  
Vol 88-88 (3-4) ◽  
pp. 441-448 ◽  
Author(s):  
R. Rajeshwari ◽  
S. Sivaramakrishnan ◽  
R. L. Smith ◽  
N. C. Subrahmanyam
Keyword(s):  
Mitochondrial Dna ◽  
Pearl Millet ◽  
Rflp Analysis ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals A new source of cytoplasmic male sterility in pearl millet: RFLP analysis of mitochondrial DNA

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 482-486 ◽  
Author(s):  
V. Sujata ◽  
S. Sivaramakrishnan ◽  
K. N. Rai ◽  
K. Seetha
Keyword(s):  
Mitochondrial Dna ◽  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Southern Blot ◽  
Pearl Millet ◽  
Pennisetum Glaucum ◽  
Blot Hybridization ◽  
Southern Blot Hybridization ◽  
Early Gene ◽  
Male Sterile

A new source of cytoplasmic male sterility (cms) in pearl millet (Pennisetum glaucum (L.) R.Br.) derived from a half-sib progeny of the Early Gene Pool (EGP 261) and used in a male-sterile line, ICMA 90111, was compared with other known cms sources for RFLP of mitochondrial (mt) DNA. Southern blot hybridization of mtDNA from ICMA 90111 digested with several restriction enzymes and probed with homologous mtDNA clones from pearl millet and heterologous gene clones from maize and wheat revealed the RFLP patterns of ICMA 90111 distinct from others studied so far. The dendrogram of male-sterile lines constructed from the Southern blot hybridization patterns indicated that ICMA 90111 represents a separate group. Our results suggest that this source of cms is unique in several respects.Key words: Pennisetum glaucum, cytoplasmic male sterility, mitochondrial DNA, RFLP.

scholarly journals Constraints to Pearl Millet (Pennisetum glaucum) Production and Farmers’ Approaches to Striga hermonthica Management in Burkina Faso

2021 ◽  
Vol 13 (15) ◽  
pp. 8460
Author(s):  
Armel Rouamba ◽  
Hussein Shimelis ◽  
Inoussa Drabo ◽  
Mark Laing ◽  
Prakash Gangashetty ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Pearl Millet ◽  
Management Practices ◽  
Pennisetum Glaucum ◽  
Control Measures ◽  
Striga Hermonthica ◽  
Soil Conditions ◽  
Management Options ◽  
Central Plateau ◽  
South Central

Pearl millet (Pennisetum glaucum) is a staple food crop in Burkina Faso that is widely grown in the Sahelian and Sudano-Sahelian zones, characterised by poor soil conditions and erratic rainfall, and high temperatures. The objective of this study was to document farmers’ perceptions of the prevailing constraints affecting pearl millet production and related approaches to manage the parasitic weeds S. hermonthica. The study was conducted in the Sahel, Sudano-Sahelian zones in the North, North Central, West Central, Central Plateau, and South Central of Burkina Faso. Data were collected through a structured questionnaire and focus group discussions involving 492 participant farmers. Recurrent drought, S. hermonthica infestation, shortage of labour, lack of fertilisers, lack of cash, and the use of low-yielding varieties were the main challenges hindering pearl millet production in the study areas. The majority of the respondents (40%) ranked S. hermonthica infestation as the primary constraint affecting pearl millet production. Respondent farmers reported yield losses of up to 80% due to S. hermonthica infestation. 61.4% of the respondents in the study areas had achieved a mean pearl millet yields of <1 t/ha. Poor access and the high cost of introduced seed, and a lack of farmers preferred traits in the existing introduced pearl millet varieties were the main reasons for their low adoption, as reported by 32% of respondents. S. hermonthica management options in pearl millet production fields included moisture conservation using terraces, manual hoeing, hand weeding, use of microplots locally referred to as ‘zaï’, crop rotation and mulching. These management techniques were ineffective because they do not suppress the below ground S. hermonthica seed, and they are difficult to implement. Integrated management practices employing breeding for S. hermonthica resistant varieties with the aforementioned control measures could offer a sustainable solution for S. hermonthica management and improved pearl millet productivity in Burkina Faso.

scholarly journals Effects of Cover Crops, Compost, and Vermicompost on Strawberry Yields and Nitrogen Availability in North Carolina

2016 ◽  
Vol 26 (5) ◽  
pp. 604-613 ◽  
Author(s):  
John E. Beck ◽  
Michelle S. Schroeder-Moreno ◽  
Gina E. Fernandez ◽  
Julie M. Grossman ◽  
Nancy G. Creamer
Keyword(s):  
North Carolina ◽  
Pearl Millet ◽  
Cover Crops ◽  
Cover Crop ◽  
Crop Yields ◽  
Pennisetum Glaucum ◽  
Organic Production ◽  
Organic N ◽  
N Availability ◽  
Soil N

Summer cover crop rotations, compost, and vermicompost additions can be important strategies for transition to organic production that can provide various benefits to crop yields, nitrogen (N) availability, and overall soil health, yet are underused in strawberry (Fragaria ×ananassa) production in North Carolina. This study was aimed at evaluating six summer cover crop treatments including pearl millet (Pennisetum glaucum), soybean (Glycine max), cowpea (Vigna unguiculata), pearl millet/soybean combination, pearl millet/cowpea combination, and a no cover crop control, with and without vermicompost additions for their effects on strawberry growth, yields, nutrient uptake, weeds, and soil inorganic nitrate-nitrogen and ammonium-nitrogen in a 2-year field experiment. Compost was additionally applied before seeding cover crops and preplant N fertilizer was reduced by 67% to account for organic N additions. Although all cover crops (with compost) increased soil N levels during strawberry growth compared with the no cover crop treatment, cover crops did not impact strawberry yields in the first year of the study. In the 2nd year, pearl millet cover crop treatments reduced total and marketable strawberry yields, and soybean treatments reduced marketable strawberry yields when compared with the no cover crop treatment, whereas vermicompost additions increased strawberry biomass and yields. Results from this study suggest that vermicompost additions can be important sustainable soil management strategies for transitional and certified organic strawberry production. Summer cover crops integrated with composts can provide considerable soil N, reducing fertilizer needs, but have variable responses on strawberry depending on the specific cover crop species or combination. Moreover, these practices are suitable for both organic and conventional strawberry growers and will benefit from longer-term studies that assess these practices individually and in combination and other benefits in addition to yields.

Identification of Hake Species (MerlucciusGenus) Using Sequencing and PCR−RFLP Analysis of Mitochondrial DNA Control Region Sequences

2001 ◽  
Vol 49 (11) ◽  
pp. 5108-5114 ◽  
Author(s):  
Javier Quinteiro ◽  
Rodrigo Vidal ◽  
Mónica Izquierdo ◽  
Carmen G. Sotelo ◽  
María José Chapela ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Control Region ◽  
Rflp Analysis ◽  
Mitochondrial Dna Control Region ◽  
Control Region Sequences ◽  
Pcr Rflp
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