Ultrasound Mediated Delivery of Compounds into Petunia Protoplasts and Cells

1995 ◽  
Vol 4 (1) ◽  
pp. 37-39 ◽  
Author(s):  
M. L. Choudhary ◽  
Chee Kok Chin
Keyword(s):  
Petunia Protoplasts

scholarly journals Regulation of Activator/Dissociation Transposition by Replication and DNA Methylation

Genetics ◽  
2001 ◽  
Vol 157 (4) ◽  
pp. 1723-1733
Author(s):  
Francesca Ros ◽  
Reinhard Kunze
Keyword(s):  
Dna Methylation ◽  
Transposable Elements ◽  
Strong Evidence ◽  
Complementary Strand ◽  
Regulatory Effect ◽  
Ds Excision ◽  
Dna Strand ◽  
Petunia Protoplasts ◽  
Ac Transposase ◽  
High Binding Affinity

Abstract In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly after replication from one of the two resulting chromatids (“chromatid selectivity”). A model has been suggested that explains this phenomenon as a consequence of different affinity for Ac transposase binding to holo-, hemi-, and unmethylated transposon ends. Here we demonstrate that in petunia cells a holomethylated Ds is unable to excise from a nonreplicating vector and that replication restores excision. A Ds element hemi-methylated on one DNA strand transposes in the absence of replication, whereas hemi-methylation of the complementary strand causes a >6.3-fold inhibition of Ds excision. Consistently in the active hemi-methylated state, the Ds ends have a high binding affinity for the transposase, whereas binding to inactive ends is strongly reduced. These results provide strong evidence for the above-mentioned model. Moreover, in the absence of DNA methylation, replication enhances Ds transposition in petunia protoplasts >8-fold and promotes formation of a predominant excision footprint. Accordingly, replication also has a methylation-independent regulatory effect on transposition.

Cell cycle regulation by plant growth regulators: involvement of auxin and cytokinin in the re-entry of Petunia protoplasts into the cell cycle

Planta ◽  
1998 ◽  
Vol 206 (2) ◽  
pp. 215-224 ◽  
Author(s):  
Christophe Tréhin ◽  
Séverine Planchais ◽  
Nathalie Glab ◽  
Claudette Perennes ◽  
James Tregear ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Cell Cycle Regulation ◽  
Petunia Protoplasts ◽  
Cycle Regulation

Ti Plasmid Homologous Sequences Present in Tissues from Agrobacterium Plasmid-transformed Petunia Protoplasts

1982 ◽  
Vol 23 (3) ◽  
pp. 451-458 ◽  
Author(s):  
J. Draper ◽  
M. R. Davey ◽  
J. P. Freeman ◽  
E. C. Cocking ◽  
B. J. Cox
Keyword(s):  
Ti Plasmid ◽  
Homologous Sequences ◽  
Petunia Protoplasts

scholarly journals Polyamine Binding to Proteins in Oat and Petunia Protoplasts

1989 ◽  
Vol 91 (2) ◽  
pp. 738-743 ◽  
Author(s):  
Yosef Mizrahi ◽  
Philip B. Applewhite ◽  
Arthur W. Galston
Keyword(s):  
Petunia Protoplasts

Detection of Silver Nanoparticles Internalization into Petunia, Petunia hybrida, Isolated Protoplasts

2012 ◽  
Vol 622-623 ◽  
pp. 878-882
Author(s):  
Roohangiz Naderi ◽  
Sarah Jafari Rad ◽  
Aliakbar Saboor Yaraghi ◽  
Mehdi Farhoodi ◽  
Mina Nemati
Keyword(s):  
Electron Microscopy ◽  
Drug Delivery ◽  
Silver Nanoparticles ◽  
Cell Membrane ◽  
Clinical Diagnosis ◽  
Petunia Hybrida ◽  
Nano Particles ◽  
Isolated Protoplasts ◽  
Long Period ◽  
Petunia Protoplasts

The use of nanoparticles has spannded many divisions of biological fields including sensors, drug delivery and clinical diagnosis. These functions all based on the most important action in cells called Endocytosis. The study of endocytosis in higher order plants remained inactive for a long period of time. In this work we use Silver Nano Particles (S.N.Ps) as a endocytosic substances were incubated by isolated protoplast of petunia .Concurrently, with the study of internalization, their appear aggregation inter and intracellular were investigated by Transmissions Electron Microscopy (TEM). Results and observations showed that the S.N.Ps crossed the cell membrane and internalized in to the cells. It can be assumed that the particles with the organic functionality – PEG coated – were internalized by the petunia protoplasts.

Sign in / Sign up

Export Citation Format

Share Document