Genotyping of the MTHFR Gene Polymorphism, C677T in Patients with Leukemia by Melting Curve Analysis

2003 ◽  
Vol 7 (3) ◽  
pp. 181-185 ◽  
Author(s):  
Ugur Deligezer ◽  
Ebru Akisik ◽  
Nejat Dalay
Keyword(s):  
Gene Polymorphism ◽  
Melting Curve ◽  
Mthfr Gene ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
Mthfr Gene Polymorphism

High-resolution melting curve analysis for genotyping of common SNP in MTHFR gene using fixed-cell suspension

2008 ◽  
Vol 22 (5-6) ◽  
pp. 329-332 ◽  
Author(s):  
Thivaratana Sinthuwiwat ◽  
Phanasit Poowasanpetch ◽  
Angsana Wongngamrungroj ◽  
Somying Promso ◽  
Chirayu Auewarakul ◽  
...  
Keyword(s):  
High Resolution ◽  
Cell Suspension ◽  
High Resolution Melting ◽  
Melting Curve ◽  
Mthfr Gene ◽  
Curve Analysis ◽  
Melting Curve Analysis ◽  
High Resolution Melting Curve ◽  
Fixed Cell

MTHFR Gene Polymorphism and Diabetic Retinopathy

2006 ◽  
Vol 2 (4) ◽  
pp. 467-476 ◽  
Author(s):  
Bentham Science Publisher Bentham Science Publisher
Keyword(s):  
Diabetic Retinopathy ◽  
Gene Polymorphism ◽  
Mthfr Gene ◽  
Mthfr Gene Polymorphism

scholarly journals Rapid genotypic detection ofBacillus anthracisand theBacillus cereusgroup by multiplex real-time PCR melting curve analysis

2005 ◽  
Vol 43 (2) ◽  
pp. 301-310 ◽  
Author(s):  
Kijeong Kim ◽  
Juwon Seo ◽  
Katherine Wheeler ◽  
Chulmin Park ◽  
Daewhan Kim ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Melting Curve ◽  
Curve Analysis ◽  
Melting Curve Analysis

scholarly journals Diagnosis of α-1-Antitrypsin Deficiency: An Algorithm of Quantification, Genotyping, and Phenotyping

2006 ◽  
Vol 52 (12) ◽  
pp. 2236-2242 ◽  
Author(s):  
Melissa R Snyder ◽  
Jerry A Katzmann ◽  
Malinda L Butz ◽  
Ping Yang ◽  
D Brian Dawson ◽  
...  
Keyword(s):  
Laboratory Testing ◽  
Null Allele ◽  
Melting Curve ◽  
Curve Analysis ◽  
Laboratory Evaluation ◽  
Melting Curve Analysis ◽  
Genotype Assay ◽  
Antitrypsin Deficiency ◽  
Phenotype Analysis ◽  
A1at Deficiency

Abstract Background: Laboratory testing in suspected α-1-antitrypsin (A1AT) deficiency involves analysis of A1AT concentrations and identification of specific alleles by genotyping or phenotyping. The purpose of this study was to define and evaluate a strategy that provides reliable laboratory evaluation of A1AT deficiency. Methods: Samples from 512 individuals referred for A1AT phenotype analysis were analyzed by quantification, phenotype, and genotype. A1AT concentrations were measured by nephelometry. Phenotype analysis was performed by isoelectric focusing electrophoresis. The genotype assay detected the S and Z deficiency alleles by a melting curve analysis. Results: Of the 512 samples analyzed, 2% of the phenotype and genotype results were discordant. Among these 10 discordant results, 7 were attributed to phenotyping errors. On the basis of these data we formulated an algorithm, according to which we analyzed samples by genotyping and quantification assays, with a reflex to phenotyping when the genotype and quantification results were not concordant. Retrospective analyses demonstrated that 4% of samples submitted for genotype and quantitative analysis were reflexed to phenotyping. Of the reflexed samples, phenotyping confirmed the genotype result in 85% of cases. In the remaining 15%, phenotyping provided further information, including identifying rare deficiency alleles and suggesting the presence of a null allele, and allowed for a more definitive interpretation of the genotype result. Conclusions: The combination of genotyping and quantification, with a reflex to phenotyping, is the optimal strategy for the laboratory evaluation of A1AT deficiency.

scholarly journals Cost-effective and fast KIR gene-content genotyping by multiplex melting curve analysis

HLA ◽  
2018 ◽  
Vol 92 (6) ◽  
pp. 384-391
Author(s):  
Leonardo M. Amorim ◽  
Tiago H. S. Santos ◽  
Jill A. Hollenbach ◽  
Paul J. Norman ◽  
Wesley M. Marin ◽  
...  
Keyword(s):  
Melting Curve ◽  
Cost Effective ◽  
Curve Analysis ◽  
Gene Content ◽  
Melting Curve Analysis ◽  
Kir Gene
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