Detection of Epidermal Growth Factor Receptor Mutations in a Few Cancer Cells from Transbronchial Cytologic Specimens by Reverse Transcriptase-Polymerase Chain Reaction

2011 ◽  
Vol 15 (6) ◽  
pp. 353-359 ◽  
Author(s):  
Nobuhiro Kanaji ◽  
Shuji Bandoh ◽  
Tomoya Ishii ◽  
Yoshio Kushida ◽  
Reiji Haba ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Reverse Transcriptase ◽  
Cancer Cells ◽  
Growth Factor Receptor ◽  
Chain Reaction ◽  
Epidermal Growth ◽  
Polymerase Chain

scholarly journals Exosomes as a biomarker platform for detecting epidermal growth factor receptor–positive high-grade gliomas

2018 ◽  
Vol 128 (4) ◽  
pp. 1091-1101 ◽  
Author(s):  
Sasidhar Venkata Manda ◽  
Yogesh Kataria ◽  
Babul Reddy Tatireddy ◽  
Balasubramaniam Ramakrishnan ◽  
Boola Gnana Ratnam ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Epidermal Growth Factor Receptor ◽  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Factor Receptor ◽  
High Grade ◽  
Chain Reaction ◽  
Epidermal Growth ◽  
Polymerase Chain ◽  
Paired Serum

OBJECTIVEHigh-grade glial brain tumors are often characterized by an elevated expression of the tumorigenic epidermal growth factor receptor variant III (EGFRvIII). The authors sought to establish a clinically adaptive protocol as a noninvasive diagnostic tool for EGFRvIII detection through serum exosomes.METHODSPurity of serum exosome/RNA was confirmed by electron microscopy and flow cytometry and through an RNA bioanalyzer profile. EGFRvIII amplification was initially established by semiquantitative polymerase chain reaction in tumor tissues and exosomes. Diagnostic performance of EGFRvIII transcript in tissue versus exosome was determined using a 2 × 2 clinical table approach. Overall survival was determined using Kaplan-Meier analysis.RESULTSThe EGFRvIII transcript was detected in 39.5% of tumor tissue samples and in 44.7% of their paired serum exosome samples; 28.1% of biopsy tumors coexpressed wild-type EGFR and EGFRvIII. Tissue EGFRvIII amplification served as the reference-positive control for its paired serum expression. The overall clinical sensitivity and specificity of semiquantitative exosome EGFRvIII polymerase chain reaction detection assay in serum were 81.58% (95% CI 65.67%–92.26%) and 79.31% (95% CI 66.65%–88.83%), respectively. Age, sex, tumor location, and side of the body on which the tumor was located had no effect on the detection rate of exosomal EGFRvIII transcript. EGFRvIII expression either in exosomes or tissue correlated with poor survival.CONCLUSIONSThe authors established a serum-based method for detection of EGFRvIII in high-grade brain tumors that might serve as an optimal noninvasive method for diagnosing EGFRvIII-positive high-grade gliomas.

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