Development of an in vitro reporter gene assay to assess xenobiotic induction of the human CYP3A4 gene

1997 ◽  
Vol 22 (4) ◽  
pp. 311-313 ◽  
Author(s):  
M. S. Ogg ◽  
T. J. B. Gray ◽  
G. G. Gibson
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay ◽  
Cyp3a4 Gene ◽  
Xenobiotic Induction

scholarly journals In vitro bioanalytical assessment of toxicity of wetland samples from Spanish Mediterranean coastline

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Alberto Celma ◽  
Geeta Mandava ◽  
Agneta Oskarsson ◽  
Juan Vicente Sancho ◽  
Lubertus Bijlsma ◽  
...  
Keyword(s):  
Water Quality ◽  
Reporter Gene ◽  
Biological Activities ◽  
Reporter Gene Assay ◽  
Water Bodies ◽  
Good Tool ◽  
Adverse Health Effects ◽  
Aryl Hydrocarbon ◽  
Gene Assay

Abstract Background Fresh water bodies represent less than 1% of overall amount of water on earth and ensuring their quality and sustainability is pivotal. Although several campaigns have been performed to monitor the occurrence of micropollutants by means of chemical analysis, this might not cover the whole set of chemicals present in the sample nor the potential toxic effects of mixtures of natural and anthropogenic chemicals. In this sense, by selecting relevant toxicity endpoints when performing in vitro bioanalysis, effect-based methodologies can be of help to perform a comprehensive assessment of water quality and reveal biological activities relevant to adverse health effects. However, no prior bioanalytical study was performed in wetland water samples from the Spanish Mediterranean coastline. Methods Eleven samples from relevant water bodies from the Spanish Mediterranean coastline were collected to monitor water quality on 8 toxicity endpoints. Aryl hydrocarbon receptor (AhR), androgenicity (AR+ and AR−), estrogenicity (ER+ and ER−), oxidative stress response (Nrf2) and vitamin D receptor (VDR+ and VDR−) reporter gene assays were evaluated. Results AhR was the reporter gene assay showing a more frequent response over the set of samples (activated by 9 out of 11 samples), with TCDD-eq in the range 7.7–22.2 pM. For AR, ER and VDR assays sporadic activations were observed. Moreover, no activity was observed on the Nrf2 reporter gene assay. Wastewater and street runaway streams from Valencia could be responsible for enhanced activities in one of the water inputs in the Natural Park ‘L’Albufera’. Conclusions Water quality of relevant wetlands from the Spanish Mediterranean coastline has been evaluated. The utilization of a panel of 5 different bioassays to cover for different toxicity endpoints has demonstrated to be a good tool to assess water quality.

Evaluation of estrogenic activities of pesticides using an in vitro reporter gene assay

2005 ◽  
Vol 15 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Mihoko Kojima ◽  
Kenji Fukunaga * ◽  
Mari Sasaki ◽  
Masafumi Nakamura ◽  
Motohiro Tsuji ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Reporter Gene Assay ◽  
Gene Assay

Evaluation of Estrogenic Activity of Wastewater: Comparison Among In Vitro ERα Reporter Gene Assay, In Vivo Vitellogenin Induction, and Chemical Analysis

2015 ◽  
Vol 49 (10) ◽  
pp. 6319-6326 ◽  
Author(s):  
Masaru Ihara ◽  
Tomokazu Kitamura ◽  
Vimal Kumar ◽  
Chang-Beom Park ◽  
Mariko O. Ihara ◽  
...  
Keyword(s):  
Chemical Analysis ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay

In vitro Estrogenicity of Resin Composites

2004 ◽  
Vol 83 (3) ◽  
pp. 222-226 ◽  
Author(s):  
H. Wada ◽  
H. Tarumi ◽  
S. Imazato ◽  
M. Narimatsu ◽  
S. Ebisu
Keyword(s):  
Bisphenol A ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Resin Composites ◽  
Minimum Concentration ◽  
Tert Butyl ◽  
Gene Assay

Previously, we have reported that sealants incorporating bisphenol A dimethacrylate showed estrogenicity by a reporter gene assay. This study tested the hypothesis that commercial composites, which contain various monomers and additives, exhibit estrogenic activity in vitro. The estrogenic activities of eluates obtained from 24 composites and 18 chemicals identified from the composites tested were examined with the use of the reporter gene assay. Among the 24 composites, 6 products were estrogenic, and among the 18 constituents, 1 photostabilizer, 2-hydroxy-4-methoxy-benzophenone (HMBP), 1 photoinitiator, 2,2-dimethoxy-2-phenyl-acetophenone (DMPA), and 1 inhibitor, 2,6-di- tert-butyl- p-cresol (BHT) had significant estrogenic activity. The concentration of HMBP in 4 estrogenic eluates was greater than the minimum concentration required for estrogenicity, and DMPA was found at a higher level than the minimum estrogenic concentration in the remaining 2 estrogenic specimens. These results suggest that the observed estrogenic activity of 6 composites is associated with the elution of either HMBP or DMPA.

An in Vitro Reporter Gene Assay Method Incorporating Metabolic Activation with Human and Rat S9 or Liver Microsomes

2001 ◽  
Vol 280 (1) ◽  
pp. 85-91 ◽  
Author(s):  
Kayo Sumida ◽  
Norihisa Ooe ◽  
Hirohisa Nagahori ◽  
Koichi Saito ◽  
Naohiko Isobe ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Liver Microsomes ◽  
Metabolic Activation ◽  
Reporter Gene Assay ◽  
Assay Method ◽  
Gene Assay

Establishment of an in vitro reporter gene assay for developmental cardiac toxicity

2001 ◽  
Vol 15 (3) ◽  
pp. 215-223 ◽  
Author(s):  
S Bremer ◽  
A.P Worth ◽  
M Paparella ◽  
K Bigot ◽  
E Kolossov ◽  
...  
Keyword(s):  
Reporter Gene ◽  
Cardiac Toxicity ◽  
Reporter Gene Assay ◽  
Gene Assay

Assessment of Polystyrene Extract for Estrogenic Activity in the Rat Uterotrophic Model and an in Vitro Recombinant Receptor Reporter Gene Assay

1998 ◽  
Vol 21 (sup1) ◽  
pp. 101-121 ◽  
Author(s):  
Patricia A. Fail ◽  
John W. Hines ◽  
Tim Zacharewski ◽  
Zhi Fen Wu ◽  
Lester Borodinsky
Keyword(s):  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Recombinant Receptor ◽  
Gene Assay

scholarly journals Natural therapies from Costa Rica for the management of menopause: Estrogenic activity in the in vitro ER‐CALUX reporter gene assay

2007 ◽  
Vol 21 (5) ◽  
Author(s):  
Brian Joseph Doyle ◽  
Tracie D. Locklear ◽  
Alice Perez ◽  
Juan Carlos Brenes ◽  
Jorge Laurito‐Gomez ◽  
...  
Keyword(s):  
Costa Rica ◽  
Reporter Gene ◽  
Estrogenic Activity ◽  
Reporter Gene Assay ◽  
Gene Assay
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