Inhibitory effect of a modified adenovirus type 5E1A gene on the NF-κB activity in porcine aortic endothelial cells induced by TNF-α

2003 ◽  
Vol 48 (1) ◽  
pp. 82-86
Author(s):  
Yewei Ma ◽  
Xiaoshan Zhou ◽  
Qingzheng Zhao ◽  
Jun Yang ◽  
Xin Gao ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Adenovirus Type ◽  
Inhibitory Effect ◽  
Aortic Endothelial Cells ◽  
Tnf Α ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

scholarly journals TNF-α, IL-4, and IFN-γ Regulate Differential Expression of P- and E-Selectin Expression by Porcine Aortic Endothelial Cells

2000 ◽  
Vol 164 (6) ◽  
pp. 3309-3315 ◽  
Author(s):  
Claire J. Stocker ◽  
Katharine L. Sugars ◽  
Olivier A. Harari ◽  
R. Clive Landis ◽  
Bernard J. Morley ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Differential Expression ◽  
Aortic Endothelial Cells ◽  
Tnf Α ◽  
Ifn Γ ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

Comparative protection against oxyradicals by three flavonoids on cultured endothelial cells

1997 ◽  
Vol 75 (6) ◽  
pp. 717-720 ◽  
Author(s):  
Ling-Hua Zeng ◽  
Jun Wu ◽  
Beverly Fung ◽  
Jeffrey H Tong ◽  
Donald Mickle ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Oxygen Consumption ◽  
Xanthine Oxidase ◽  
Inhibitory Effect ◽  
Control Group ◽  
Aortic Endothelial Cells ◽  
Cytochrome C Reduction ◽  
Porcine Aortic Endothelial Cells ◽  
Aortic Endothelial

Oxygen-derived free radicals are known to injure the endothelium of aorta in diverse disorders. In this study we compared the cytoprotective effects of three flavonoids against oxyradical damage to porcine aortic endothelial cells in vitro. Cultured porcine aortic endothelial cells were exposed to oxyradicals generated by xanthine oxidase - hypoxanthine (XO-HP). The cytoprotective activities of morin, quercetin, and catechin on these systems were compared using established morphologic criteria. The results in the XO-HP system showed that morin at 0.125, 0.25, and 0.5 mM delayed cell necrosis to 27.4 ± 1.3, 46.8 ± 1.8, and longer than 70 min, respectively, compared with 12.0 ± 1.3 min in the control group. These degrees of protection were significantly stronger than those provided by quercetin and catechin at corresponding concentrations (p < 0.01). Morin and quercetin were moderate inhibitors of xanthine oxidase on the basis of the oxygen consumption rate, whereas catechin at the same concentrations had little inhibitory effect. The data from uric acid formation and cytochrome c reduction were consistent with the oxygen consumption measurement for the three flavonoids. Key words: flavonoids, oxyradicals, aortic endothelial cells.

Effects of Dipyrone on Prostaglandin Production by Human Platelets and Cultured Bovine Aortic Endothelial Cells

1983 ◽  
Vol 49 (02) ◽  
pp. 132-137 ◽  
Author(s):  
A Eldor ◽  
G Polliack ◽  
I Vlodavsky ◽  
M Levy
Keyword(s):  
Endothelial Cells ◽  
Arachidonic Acid ◽  
Competitive Inhibition ◽  
Inhibitory Effect ◽  
Human Platelets ◽  
Ionophore A23187 ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Aortic Endothelial

SummaryDipyrone and its metabolites 4-methylaminoantipyrine, 4-aminoantipyrine, 4-acetylaminoantipyrine and 4-formylaminoan- tipyrine inhibited the formation of thromboxane A2 (TXA2) during in vitro platelet aggregation induced by ADP, epinephrine, collagen, ionophore A23187 and arachidonic acid. Inhibition occurred after a short incubation (30–40 sec) and depended on the concentration of the drug or its metabolites and the aggregating agents. The minimal inhibitory concentration of dipyrone needed to completely block aggregation varied between individual donors, and related directly to the inherent capacity of their platelets to synthesize TXA2.Incubation of dipyrone with cultured bovine aortic endothelial cells resulted in a time and dose dependent inhibition of the release of prostacyclin (PGI2) into the culture medium. However, inhibition was abolished when the drug was removed from the culture, or when the cells were stimulated to produce PGI2 with either arachidonic acid or ionophore A23187.These results indicate that dipyrone exerts its inhibitory effect on prostaglandins synthesis by platelets or endothelial cells through a competitive inhibition of the cyclooxygenase system.

scholarly journals Urea transporters are distributed in endothelial cells and mediate inhibition of l-arginine transport

2002 ◽  
Vol 283 (3) ◽  
pp. F578-F582 ◽  
Author(s):  
Laszlo Wagner ◽  
Janet D. Klein ◽  
Jeff M. Sands ◽  
Chris Baylis
Keyword(s):  
Endothelial Cells ◽  
Cell Types ◽  
Inhibitory Effect ◽  
Rat Aorta ◽  
Wide Distribution ◽  
Aortic Endothelial Cells ◽  
Bovine Aortic Endothelial Cells ◽  
Elevated Blood Urea Nitrogen ◽  
Aortic Endothelial

Our laboratory previously reported that uremic levels of urea inhibitl-arginine (l-Arg) transport into endothelial cells. The present study further investigated this effect. We measuredl-Arg transport in cultured bovine aortic endothelial cells with normal or high urea (25 mM). The urea transport inhibitor phloretin abolished the inhibitory effect of urea on l-Arg transport, suggesting a role for urea transporters (UTs). We screened bovine aortic endothelial cells and several other endothelial cell types for the presence of UTs by using Western blot analysis. UT-B was present in all endothelial cells, irrespective of species or location of derivation, whereas UT-A distribution was variable and sparse. UT-B was also abundant in rat aorta, mesenteric blood vessels, and spinotrapezius muscle, whereas UT-A distribution was, again, variable and sparse. Chronic elevation of urea had variable, inconsistent effects on UT abundance. This study showed that urea must enter endothelial cells, probably by UT-B, to inhibit l-Arg transport. In view of the wide distribution of UT-B in rat vasculature, elevated blood urea nitrogen may lead to endothelial l-Arg deficiency in vivo.

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