Identification ofGluconobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses

2008 ◽  
Vol 58 (4) ◽  
pp. 741-747 ◽  
Author(s):  
Jintana Kommanee ◽  
Ancharida Akaracharanya ◽  
Somboon Tanasupawat ◽  
Taweesak Malimas ◽  
Pattaraporn Yukphan ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
23S Rrna Gene

Identification ofAcetobacter strains isolated in Thailand based on 16S–23S rRNA gene ITS restriction and 16S rRNA gene sequence analyses

2008 ◽  
Vol 58 (2) ◽  
pp. 319-324 ◽  
Author(s):  
Jintana Kommanee ◽  
Ancharida Akaracharanya ◽  
Somboon Tanasupawat ◽  
Taweesak Malimas ◽  
Pattaraporn Yukphan ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
16S Rrna Gene Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
23S Rrna Gene

Bacterial diversity of the Inner Mongolian Baer Soda Lake as revealed by 16S rRNA gene sequence analyses

Extremophiles ◽  
2004 ◽  
Vol 8 (1) ◽  
pp. 45-51 ◽  
Author(s):  
Peijin Zhou ◽  
Yanhe Ma ◽  
Weizhou Zhang ◽  
Yanfen Xue ◽  
Antonio Ventosa ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bacterial Diversity ◽  
Gene Sequence ◽  
Soda Lake ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses

scholarly journals Coinfection of Dermacentor silvarum Olenev (Acari: Ixodidae) by Coxiella-Like, Arsenophonus-Like, and Rickettsia-Like Symbionts

2013 ◽  
Vol 79 (7) ◽  
pp. 2450-2454 ◽  
Author(s):  
Limeng Liu ◽  
Lingxia Li ◽  
Jiannan Liu ◽  
Yonghong Hu ◽  
Zhao Liu ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
Sequence Analyses ◽  
Content Type

ABSTRACTWe report that multiple symbionts coexist inDermacentor silvarum. Based on 16S rRNA gene sequence analyses, we prove thatCoxiella-like andArsenophonus-like symbionts, with 95.6% and 96.7% sequence similarity to symbionts in the closest taxon, respectively, are novel. Moreover, we also provide evidence that theCoxiella-like symbiont appears to be the primary symbiont.

scholarly journals Streptomyces durmitorensis sp. nov., a producer of an FK506-like immunosuppressant

2007 ◽  
Vol 57 (9) ◽  
pp. 2119-2124 ◽  
Author(s):  
Miloje Savic ◽  
Ivana Bratic ◽  
Branka Vasiljevic
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Trees ◽  
Gene Sequence ◽  
Novel Species ◽  
Phylogenetic Analyses ◽  
16S Rrna Gene Sequence ◽  
23S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence

Screening of soil samples from the Durmitor National Park, Serbia and Montenegro, for strains producing immunosuppressants with a similar mechanism of action to FK506 resulted in the isolation of the actinomycete strain MS405T. Isolate MS405T was found to have morphological and phenotypic properties that were consistent with its classification as a Streptomyces strain. The DNA G+C content of strain MS405T was 72 mol%. 16S rRNA gene sequence data confirmed the taxonomic position of the strain, following the generation of phylogenetic trees by using various treeing algorithms. On the basis of 16S rRNA gene sequence similarity, strain MS405T was shown to belong to the Streptomyces albidoflavus ‘supercluster’, being related to Streptomyces aureus DSM 41785T (99.59 % similarity) and Streptomyces kanamyceticus DSM 40500T (99.32 %). The 16S–23S rRNA internally transcribed spacer (ITS) region exhibited variations in length and sequence composition, showing limited usefulness in phylogenetic analyses. However, DNA relatedness values support the classification of this isolate within a novel species. A number of physiological and biochemical tests distinguished strain MS405T from its closest phylogenetic neighbours. Therefore, strain MS405T represents a novel species, for which the name Streptomyces durmitorensis sp. nov. is proposed, with the type strain MS405T (=DSM 41863T =CIP 108995T).

scholarly journals Discordant 16S and 23S rRNA Gene Phylogenies for the Genus Helicobacter: Implications for Phylogenetic Inference and Systematics

2005 ◽  
Vol 187 (17) ◽  
pp. 6106-6118 ◽  
Author(s):  
Floyd E. Dewhirst ◽  
Zeli Shen ◽  
Michael S. Scimeca ◽  
Lauren N. Stokes ◽  
Tahani Boumenna ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Data ◽  
Rrna Genes ◽  
23S Rrna ◽  
Rrna Gene ◽  
Rrna Gene Sequence ◽  
23S Rrna Gene ◽  
23S Rrna Genes

ABSTRACT Analysis of 16S rRNA gene sequences has become the primary method for determining prokaryotic phylogeny. Phylogeny is currently the basis for prokaryotic systematics. Therefore, the validity of 16S rRNA gene-based phylogenetic analyses is of fundamental importance for prokaryotic systematics. Discrepancies between 16S rRNA gene analyses and DNA-DNA hybridization and phenotypic analyses have been noted in the genus Helicobacter. To clarify these discrepancies, we sequenced the 23S rRNA genes for 55 helicobacter strains representing 41 taxa (>2,700 bases per sequence). Phylogenetic-tree construction using neighbor-joining, parsimony, and maximum likelihood methods for 23S rRNA gene sequence data yielded stable trees which were consistent with other phenotypic and genotypic methods. The 16S rRNA gene sequence-derived trees were discordant with the 23S rRNA gene trees and other data. Discrepant 16S rRNA gene sequence data for the helicobacters are consistent with the horizontal transfer of 16S rRNA gene fragments and the creation of mosaic molecules with loss of phylogenetic information. These results suggest that taxonomic decisions must be supported by other phylogenetically informative macromolecules, such as the 23S rRNA gene, when 16S rRNA gene-derived phylogeny is discordant with other credible phenotypic and genotypic methods. This study found Wolinella succinogenes to branch with the unsheathed-flagellum cluster of helicobacters by 23S rRNA gene analyses and whole-genome comparisons. This study also found intervening sequences (IVSs) in the 23S rRNA genes of strains of 12 Helicobacter species. IVSs were found in helices 10, 25, and 45, as well as between helices 31′ and 27′. Simultaneous insertion of IVSs at three sites was found in H. mesocricetorum.

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