Characterization of halophilic Archaea isolated from different hypersaline ecosystems

2006 ◽  
Vol 56 (2) ◽  
pp. 119-127 ◽  
Author(s):  
Giancarlo Moschetti ◽  
Maria Aponte ◽  
Giuseppe Blaiotta ◽  
Annalisa Casaburi ◽  
Mario Chiurazzi ◽  
...  
Keyword(s):  
Halophilic Archaea ◽  
Hypersaline Ecosystems

scholarly journals Functional and Molecular Characterization of the Halomicrobium sp. IBSBa Inulosucrase

2021 ◽  
Vol 9 (4) ◽  
pp. 749
Author(s):  
Gülbahar Abaramak ◽  
Jaime Ricardo Porras-Domínguez ◽  
Henry Christopher Janse van Rensburg ◽  
Eveline Lescrinier ◽  
Ebru Toksoy Öner ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Phylogenetic Analyses ◽  
Halophilic Archaea ◽  
Maximum Activity ◽  
Physiological Relevance ◽  
Health Related ◽  
Specific Alternative ◽  
Binding Groove ◽  
Native Host

Fructans are fructose-based (poly)saccharides with inulin and levan being the best-known ones. Thanks to their health-related benefits, inulin-type fructans have been under the focus of scientific and industrial communities, though mostly represented by plant-based inulins, and rarely by microbial ones. Recently, it was discovered that some extremely halophilic Archaea are also able to synthesize fructans. Here, we describe the first in-depth functional and molecular characterization of an Archaeal inulosucrase from Halomicrobium sp. IBSBa (HmcIsc). The HmcIsc enzyme was recombinantly expressed and purified in Escherichia coli and shown to synthesize inulin as proven by nuclear magnetic resonance (NMR) analysis. In accordance with the halophilic lifestyle of its native host, the enzyme showed maximum activity at very high NaCl concentrations (3.5 M), with specific adaptations for that purpose. Phylogenetic analyses suggested that Archaeal inulosucrases have been acquired from halophilic bacilli through horizontal gene transfer, with a HX(H/F)T motif evolving further into a HXHT motif, together with a unique D residue creating the onset of a specific alternative acceptor binding groove. This work uncovers a novel area in fructan research, highlighting unexplored aspects of life in hypersaline habitats, and raising questions about the general physiological relevance of inulosucrases and their products in nature.

scholarly journals Experimental Characterization of Cis-Acting Elements Important for Translation and Transcription in Halophilic Archaea

PLoS Genetics ◽  
2007 ◽  
Vol 3 (12) ◽  
pp. e229 ◽  
Author(s):  
Mariam Brenneis ◽  
Oliver Hering ◽  
Christian Lange ◽  
Jörg Soppa
Keyword(s):  
Halophilic Archaea ◽  
Experimental Characterization ◽  
Cis Acting

scholarly journals Characterization of the proteasome from the extremely halophilic archaeonHaloarcula marismortui

Archaea ◽  
2002 ◽  
Vol 1 (1) ◽  
pp. 53-61 ◽  
Author(s):  
B. Franzetti ◽  
G. Schoehn ◽  
D. Garcia ◽  
R. W. H. Ruigrok ◽  
G. Zaccai
Keyword(s):  
Three Dimensional ◽  
Halophilic Archaea ◽  
20S Proteasome ◽  
Misfolded Protein ◽  
Haloarcula Marismortui ◽  
Low Salt ◽  
Dimensional Reconstruction ◽  
The Stability

A 20S proteasome, comprising two subunits α and β, was purified from the extreme halophilic archaeonHaloarcula marismortui, which grows only in saturated salt conditions. The three-dimensional reconstruction of theH. marismortuiproteasome (Hm proteasome), obtained from negatively stained electron micrographs, is virtually identical to the structure of a thermophilic proteasome filtered to the same resolution. The stability of the Hm proteasome was found to be less salt-dependent than that of other halophilic enzymes previously described. The proteolytic activity of the Hm proteasome was investigated using the malate dehydrogenase fromH. marismortui(HmMalDH) as a model substrate. The HmMalDH denatures when the salt concentration is decreased below 2 M. Under these conditions, the proteasome efficiently cleaves HmMalDH during its denaturation process, but the fully denatured HmMalDH is poorly degraded. These in vitro experiments show that, at low salt concentrations, the 20S proteasome from halophilic archaea eliminates a misfolded protein.

25 Characterization of Lipids of Halophilic Archaea

2006 ◽  
pp. 585-613 ◽  
Author(s):  
Angela Corcelli ◽  
Simona Lobasso
Keyword(s):  
Halophilic Archaea

scholarly journals Reconstructing the evolutionary origins of extreme halophilic Archaeal lineages

2019 ◽  
Author(s):  
Yutian Feng ◽  
Uri Neri ◽  
Sean Gosselin ◽  
Artemis S. Louyakis ◽  
R. Thane Papke ◽  
...  
Keyword(s):  
Convergent Evolution ◽  
Common Ancestor ◽  
Phylogenetic Reconstruction ◽  
Strong Support ◽  
Halophilic Archaea ◽  
Gene Families ◽  
Catalytic Subunits ◽  
Evolutionary Origins ◽  
Extremely Halophilic Archaea

AbstractInterest and controversy surrounding the evolutionary origins of extremely halophilic Archaea has increased in recent years, due to the discovery and characterization of the Nanohaloarchaea and the Methanonatronarchaeia. Initial attempts in explaining the evolutionary placement of the two new lineages in relation to the classical Halobacteria (also referred to as Haloarchaea) resulted in hypotheses that imply the new groups share a common ancestor with the Haloarchaea. However, more recent analyses have led to a shift: the Nanohaloarchaea have been largely accepted as being a member of the DPANN superphylum, outside of the euryarchaeota; while the Methanonatronarchaeia have been placed near the base of the Methanotecta (composed of the class II methanogens, the halobacteriales, and archaeoglobales). These opposing hypotheses have far-reaching implications on the concepts of convergent evolution (unrelated groups evolve similar strategies for survival), genome reduction, and gene transfer. In this work, we attempt to resolve these conflicts with phylogenetic and phylogenomic data. We provide a robust taxonomic sampling of Archaeal genomes that spans the crenarchaeota, euryarchaeota, and the DPANN superphylum. In addition, we sampled and assembled 7 new representatives of the Nanohaloarchaea, from distinct geographic locations. Phylogenies derived from these data imply the highly conserved ATP synthase catalytic/non-catalytic subunits of Nanohaloarchaea share a sisterhood relationship with the Haloarchaea. This relationship, with strong support, was also observed for several other gene families. In addition, we present and evaluate data that argue for and against the monophyly of the DPANN superphylum. We employed phylogenetic reconstruction, constrained topology tests, and gene concordance factors to explore the support for and against the monophyly of the Haloarchaea, Nanohaloarchaea, and Methanonatronarchaeia.

Characterization of extremely halophilic Archaea isolated from the Ayvalik Saltern, Turkey

2004 ◽  
Vol 20 (7) ◽  
pp. 719-725 ◽  
Author(s):  
Rahel Elevi ◽  
Parvaneh Assa ◽  
Meral Birbir ◽  
Ayse Ogan ◽  
Aharon Oren
Keyword(s):  
Halophilic Archaea ◽  
Extremely Halophilic Archaea
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