Changes of polyphosphoinositides, lysophospholipid, and free fatty acids in transient cerebral ischemia of rat brain

1990 ◽  
Vol 12 (3) ◽  
pp. 215-228 ◽  
Author(s):  
Hiroyuki Kinouchi ◽  
Shigeki Imaizumi ◽  
Takashi Yoshimoto ◽  
Hirotaka Yamamoto ◽  
Masakichi Motomiya
Keyword(s):  
Fatty Acids ◽  
Cerebral Ischemia ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Transient Cerebral Ischemia

Influence of transient ischemia on lipid-soluble antioxidants, free fatty acids and energy metabolites in rat brain

1982 ◽  
Vol 245 (2) ◽  
pp. 307-316 ◽  
Author(s):  
Shinichi Yoshida ◽  
Kouichi Abe ◽  
Raul Busto ◽  
Brant D. Watson ◽  
Kyuya Kogure ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Transient Ischemia ◽  
Energy Metabolites

scholarly journals In Vivo Microdialysis of Endogenous and 13C-labeled TCA Metabolites in Rat Brain: Reversible and Persistent Effects of Mitochondrial Inhibition and Transient Cerebral Ischemia

Metabolites ◽  
2019 ◽  
Vol 9 (10) ◽  
pp. 204 ◽  
Author(s):  
Jesper F. Havelund ◽  
Kevin H. Nygaard ◽  
Troels H. Nielsen ◽  
Carl-Henrik Nordström ◽  
Frantz R. Poulsen ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Mitochondrial Dysfunction ◽  
Rat Brain ◽  
De Novo ◽  
Tricarboxylic Acid Cycle ◽  
Experimental Models ◽  
Transient Cerebral Ischemia ◽  
Metabolic Disturbances ◽  
Metabolic Marker

Cerebral micro-dialysis allows continuous sampling of extracellular metabolites, including glucose, lactate and pyruvate. Transient ischemic events cause a rapid drop in glucose and a rise in lactate levels. Following such events, the lactate/pyruvate (L/P) ratio may remain elevated for a prolonged period of time. In neurointensive care clinics, this ratio is considered a metabolic marker of ischemia and/or mitochondrial dysfunction. Here we propose a novel, sensitive microdialysis liquid chromatography-mass spectrometry (LC-MS) approach to monitor mitochondrial dysfunction in living brain using perfusion with 13C-labeled succinate and analysis of 13C-labeled tricarboxylic acid cycle (TCA) intermediates. This approach was evaluated in rat brain using malonate-perfusion (10–50 mM) and endothelin-1 (ET-1)-induced transient cerebral ischemia. In the malonate model, the expected changes upon inhibition of succinate dehydrogenase (SDH) were observed, i.e., an increase in endogenous succinate and decreases in fumaric acid and malic acid. The inhibition was further elaborated by incorporation of 13C into specific TCA intermediates from 13C-labeled succinate. In the ET-1 model, increases in non-labeled TCA metabolites (reflecting release of intracellular compounds) and decreases in 13C-labeled TCA metabolites (reflecting inhibition of de novo synthesis) were observed. The analysis of 13C incorporation provides further layers of information to identify metabolic disturbances in experimental models and neuro-intensive care patients.

Long-chain acyl CoA's and free fatty acids in rat brain after head injury

1997 ◽  
Vol 237 ◽  
pp. S25
Author(s):  
H. Heller ◽  
J. Deutsch ◽  
A.D. Purdon ◽  
S.I. Rapoport ◽  
M. Horowitz ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Head Injury ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Chain Acyl ◽  
Long Chain

Dissecting functional phenotypes of microglia and macrophages in the rat brain after transient cerebral ischemia

Glia ◽  
2018 ◽  
Vol 67 (2) ◽  
pp. 232-245 ◽  
Author(s):  
Wenson D. Rajan ◽  
Bartosz Wojtas ◽  
Bartlomiej Gielniewski ◽  
Anna Gieryng ◽  
Malgorzata Zawadzka ◽  
...  
Keyword(s):  
Cerebral Ischemia ◽  
Rat Brain ◽  
Transient Cerebral Ischemia

Effects of transient cerebral ischemia on hsp40 mRNA levels in rat brain

1998 ◽  
Vol 55 (2) ◽  
pp. 341-344 ◽  
Author(s):  
Wulf Paschen ◽  
Thomas Linden ◽  
Jens Doutheil
Keyword(s):  
Cerebral Ischemia ◽  
Rat Brain ◽  
Transient Cerebral Ischemia ◽  
Mrna Levels

REGIONAL DISTRIBUTION AND RATE OF PRODUCTION OF FREE FATTY ACIDS IN RAT BRAIN

1971 ◽  
Vol 18 (8) ◽  
pp. 1387-1393 ◽  
Author(s):  
N. G. Bazán ◽  
Haydeé E. P. Bazán ◽  
Wendy G. Kennedy ◽  
C. D. Joel
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Regional Distribution

scholarly journals Effect of mild hypothermia on ischemia-induced release of neurotransmitters and free fatty acids in rat brain.

Stroke ◽  
1989 ◽  
Vol 20 (7) ◽  
pp. 904-910 ◽  
Author(s):  
R Busto ◽  
M Y Globus ◽  
W D Dietrich ◽  
E Martinez ◽  
I Valdés ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Free Fatty Acids ◽  
Rat Brain ◽  
Mild Hypothermia
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