The determination of true decay time of fluorescence of dye solutions at a very low concentration

1977 ◽  
Vol 43 (2) ◽  
pp. 109-115
Author(s):  
I. Ketskeméty ◽  
L. Gáti ◽  
A. Győri ◽  
L. Kozma
1975 ◽  
Vol 63 (1) ◽  
pp. 107-116 ◽  
Author(s):  
Katalin Z. Balla ◽  
Etelka G. Hars�nyi ◽  
L�szl� P�los ◽  
Ern� Pungor

1989 ◽  
Vol 104 (2) ◽  
pp. 123-126
Author(s):  
R. Mewe ◽  
G.H.J. van den Oord ◽  
J. Jakimiec

AbstractWe have re-analyzed the X-ray flare on Algol which was observed with EXOSAT (White et al. (1986)). The common practice of estimating loop volume and length from the decay time of the flare is discussed extensively. We show that during the decay phase of the flare both scaling laws for coronal loops are valid. This implies a unique determination of loop volume and length and allows a check whether additional heating occurs in the decay phase of a flare.


2004 ◽  
Vol 9 (8) ◽  
pp. 726-733 ◽  
Author(s):  
Heidrun Rhode ◽  
Margarete Schulze ◽  
Simon Renard ◽  
Peter Zimmermann ◽  
Thomas Moore ◽  
...  

An efficient method is presented to determine precision and accuracy of multichannel liquid-handling systems under conditions near to application. Themethod consists of gravimetrical determination of accuracy and optical determination of precision based on the dilution of absorbing and fluorescent dye solutions in microplates. Mean delivery volume per well can be determined with precision better than a 0.04% coefficient of variation (CV). Optical signal precision, CV( S), is improved by multiwavelength measurements. Precision of absorbance measurement yields a better resolution than precision of fluorescence measurement (0.3% and 1.5%, respectively), indicating that absorbance measurements should be preferred. From CV( S), an upper bound of the precision of the volumes delivered is derived. Method performance is demonstrated with the dispenser CyBi™-Drop and the pipettor CyBi™-Well using different ejection principles; with commonly used fluids; with 96-, 384-, and 1536-well microplates; and with photometric and fluorometric indicators. Precision of the volumes delivered, as obtained with optimized methods, all plate formats, and both devices, is better than 2% CV with 2 µ L set volume and about 1% CV with higher set volumes.


Sign in / Sign up

Export Citation Format

Share Document