Rhythmic oscillations in wheat chloroplast photochemical activity. II. Further characterization of the rhythm in photosystem II photoelectron transport activity

1988 ◽  
Vol 98 (3) ◽  
pp. 175-181
Author(s):  
Shabbir A Sayeed ◽  
Prasanna Mohanty
Keyword(s):  
Photosystem Ii ◽  
Photochemical Activity ◽  
Transport Activity

Characterization of photosystem II with the atomic-force microscope

1992 ◽  
Vol 50 (2) ◽  
pp. 1146-1147
Author(s):  
Kathleen M. Marr ◽  
Mary K. Lyon
Keyword(s):  
Photosystem Ii ◽  
Atomic Force Microscope ◽  
Three Dimensional ◽  
Biologically Active ◽  
Atomic Force ◽  
Freeze Etching ◽  
Image Averaging ◽  
Oxygen Evolving ◽  
Averaging Techniques

Photosystem II (PSII) is different from all other reaction centers in that it splits water to evolve oxygen and hydrogen ions. This unique ability to evolve oxygen is partly due to three oxygen evolving polypeptides (OEPs) associated with the PSII complex. Freeze etching on grana derived insideout membranes revealed that the OEPs contribute to the observed tetrameric nature of the PSIl particle; when the OEPs are removed, a distinct dimer emerges. Thus, the surface of the PSII complex changes dramatically upon removal of these polypeptides. The atomic force microscope (AFM) is ideal for examining surface topography. The instrument provides a topographical view of individual PSII complexes, giving relatively high resolution three-dimensional information without image averaging techniques. In addition, the use of a fluid cell allows a biologically active sample to be maintained under fully hydrated and physiologically buffered conditions. The OEPs associated with PSII may be sequentially removed, thereby changing the surface of the complex by one polypeptide at a time.

Characterization of a resolved oxygen-evolving Photosystem II preparation from spinach thylakoids

1983 ◽  
Vol 724 (2) ◽  
pp. 201-211 ◽  
Author(s):  
Eric Lam ◽  
Barbara Baltimore ◽  
William Ortiz ◽  
Susan Chollar ◽  
Anastasios Melis ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Oxygen Evolving ◽  
Spinach Thylakoids

scholarly journals Cloning of the psbK gene from Synechocystis sp. PCC 6803 and characterization of photosystem II in mutants lacking PSII-K

1991 ◽  
Vol 266 (17) ◽  
pp. 11111-11115
Author(s):  
M. Ikeuchi ◽  
B. Eggers ◽  
G.Z. Shen ◽  
A. Webber ◽  
J.J. Yu ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Synechocystis Sp ◽  
Pcc 6803

scholarly journals Compositional, structural, and surface characterization of heat-treated halloysite samples: Influence of surface treatment on photochemical activity

2021 ◽  
Vol 212 ◽  
pp. 106222
Author(s):  
Balázs Zsirka ◽  
Veronika Vágvölgyi ◽  
Katalin Győrfi ◽  
Erzsébet Horváth ◽  
Róbert K. Szilágyi ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Surface Characterization ◽  
Photochemical Activity ◽  
Heat Treated

Photoinhibition of Electron Transport Activity of Photosystem II in Isolated Thylakoids Studied by Thermoluminescence and Delayed Luminescence

1988 ◽  
Vol 43 (11-12) ◽  
pp. 871-876 ◽  
Author(s):  
Imre Vass ◽  
Narendranath Mohanty ◽  
Sándor Demeter
Keyword(s):  
Electron Transport ◽  
Photosystem Ii ◽  
Photosystem I ◽  
Half Life ◽  
Delayed Luminescence ◽  
Transport Activity ◽  
Primary Target ◽  
Electron Transport Activity ◽  
The Stability ◽  
Spinach Thylakoids

Abstract The effect of photoinhibition on the primary (QA) and secondary (QB) quinone acceptors of photosystem I I was investigated in isolated spinach thylakoids by the methods of thermoluminescence and delayed luminescence. The amplitudes of the Q (at about 2 °C) and B (at about 30 °C) thermoluminescence bands which are associated with the recombination of the S2QA- and S2QB charge pairs, respectively, exhibited parallel decay courses during photoinhibitory treatment. Similarly, the amplitudes of the flash-induced delayed luminescence components ascribed to the recombination of S20A and S2OB charge pairs and having half life-times of about 3 s and 30 s, respectively, declined in parallel with the amplitudes of the corresponding Q and B thermoluminescence bands. The course of inhibition of thermoluminescence and delayed luminescence intensity was parallel with that of the rate of oxygen evolution. The peak positions of the B and Q thermoluminescence bands as well as the half life-times of the corresponding delayed luminescence components were not affected by photoinhibition. These results indicate that in isolated thylakoids neither the amount nor the stability of the reduced OB acceptor is preferentially decreased by photoinhibition. We conclude that either the primary target of photodamage is located before the O b binding site in the reaction center of photosystem II or QA and OB undergo simultaneous damage.

Characterization of bonding interactions of the intermediary electron acceptor in the reaction center of Photosystem II by FTIR spectroscopy

1990 ◽  
Vol 1016 (1) ◽  
pp. 49-54 ◽  
Author(s):  
E. Nabedryk ◽  
S. Andrianambinintsoa ◽  
G. Berger ◽  
M. Leonhard ◽  
W. Mäntele ◽  
...  
Keyword(s):  
Photosystem Ii ◽  
Ftir Spectroscopy ◽  
Reaction Center ◽  
Electron Acceptor

Characterization of the gene encoding the 10 kDa polypeptide of photosystem II fromArabidopsis thaliana

1991 ◽  
Vol 17 (3) ◽  
pp. 517-522 ◽  
Author(s):  
Gabriel Gil-G�mez ◽  
Pedro F. Marrero ◽  
Diego Haro ◽  
Jos� Ayt� ◽  
Fausto G. Hegardt
Keyword(s):  
Photosystem Ii ◽  
Gene Encoding
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