Screening for powdery mildew (Erysiphae polygoni DC.) resistance in mungbean (Vigna radiata (L.) Wilczek) using excised leaves

1987 ◽  
Vol 97 (5) ◽  
pp. 365-369
Author(s):  
K S Reddy ◽  
S E Pawar ◽  
C R Bhatia
Keyword(s):  
Powdery Mildew ◽  
Vigna Radiata ◽  
Dc Resistance

Identification of a major locus conferring resistance to powdery mildew (Erysiphe polygoni DC) in mungbean (Vigna radiata L. Wilczek) by QTL analysis

Genome ◽  
2003 ◽  
Vol 46 (5) ◽  
pp. 738-744 ◽  
Author(s):  
M E Humphry ◽  
T Magner ◽  
C L McIntyre ◽  
E A.B Aitken ◽  
C J Liu
Keyword(s):  
Powdery Mildew ◽  
Linkage Map ◽  
Qtl Analysis ◽  
Vigna Radiata ◽  
Mapping Population ◽  
Susceptible Variety ◽  
Resistance Response ◽  
Causal Organism ◽  
Major Locus ◽  
Erysiphe Polygoni

A major locus conferring resistance to the causal organism of powdery mildew, Erysiphe polygoni DC, in mungbean (Vigna radiata L. Wilczek) was identified using QTL analysis with a population of 147 recombinant inbred individuals. The population was derived from a cross between 'Berken', a highly susceptible variety, and ATF 3640, a highly resistant line. To test for response to powdery mildew, F7 and F8 lines were inoculated by dispersing decaying mungbean leaves with residual conidia of E. polygoni amongst the young plants to create an artificial epidemic and assayed in a glasshouse facility. To generate a linkage map, 322 RFLP clones were tested against the two parents and 51 of these were selected to screen the mapping population. The 51 probes generated 52 mapped loci, which were used to construct a linkage map spanning 350 cM of the mungbean genome over 10 linkage groups. Using these markers, a single locus was identified that explained up to a maximum of 86% of the total variation in the resistance response to the pathogen.Key words: mungbean, powdery mildew, Erysiphe polygoni, QTL, molecular markers.

Development of PCR-based markers for a major locus conferring powdery mildew resistance in mungbean (Vigna radiata)

2008 ◽  
Vol 127 (4) ◽  
pp. 429-432 ◽  
Author(s):  
M. C. Zhang ◽  
D. M. Wang ◽  
Z. Zheng ◽  
M. Humphry ◽  
C. J. Liu
Keyword(s):  
Powdery Mildew ◽  
Vigna Radiata ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Major Locus

scholarly journals First Report of Podosphaera xanthii Causing Powdery Mildew on Mungbean (Vigna radiata) in Taiwan

Plant Disease ◽  
2021 ◽  
Author(s):  
Zong-ming Sheu ◽  
Ming-hsueh Chiu ◽  
Lawrence Kenyon
Keyword(s):  
Powdery Mildew ◽  
Vigna Radiata ◽  
Morphological Characteristics ◽  
Resistance Breeding ◽  
Its Region ◽  
Infected Leaf ◽  
Pathogenicity Test ◽  
Causal Organism ◽  
First Report ◽  
Powdery Mildew Pathogen

Mungbean (Vigna radiata L.) is routinely grown in the experimental fields at the headquarters of the World Vegetable Center (23°6'30.88"N, 120°17'51.31"E) for breeding, research and germplasm multiplication. In a spring 2016 mungbean trial, about 50% of the plants were affected with powdery mildew. The white, powdery-like patches first appeared on the upper leaf surfaces, and soon developed to grey patches on both sides of the leaves. Purple to brown discoloration appeared on the underside of the infected leaf. Microscopy examination revealed that the causal organism was not Erysiphe polygoni, which had previously been documented as the powdery mildew pathogen on mungbean in Taiwan (Hartman et al. 1993). The fungus produced typical structures of the powdery mildew Euoidium, anamorph of the genus Podosphaera. The mycelium consisted of septate, flexuous hyphae with indistinct appressoria. The erect conidiophores arising from superficial hyphae varied from straight or slightly curved to curled. Three to ten conidia were borne in long chains with crenate edges. Foot-cells were straight, cylindrical and measured 30 to 52 µm long. Conidia were hyaline, ellipsoid-ovoid to barrel-shaped, with fibrosin bodies, and measured 27 to 33 (mean = 30.4) × 15 to 20 (mean = 16.6) µm. Germ tubes were clavate and occasionally forked, and were produced from the lateral sites of the conidia. No chasmothecia were found in the samples. The morphological characteristics were consistent with P. xanthii (Castagne) U. Braun & Shishkoff (Braun & Cook 2012). To confirm the identity, the internal transcribed spacer (ITS) region of rDNA and partialβ-tubulin gene (TUB2) for the isolate MG3 were amplified with the primers ITS4/ITS5 (White et al. 1990) and BtuF5/BtuR7a (Ellingham et al. 2019), respectively. BLASTn analysis revealed the ITS sequence (MN833717) was 100% identical to many records of P. xanthii whereas the TUB2 sequence (MW363957) was 100% identical to a record of P. fusca (syn. P. xanthii; KC333362) in NCBI GenBank. A pathogenicity test was conducted by dusting conidia from an infected leaf onto six healthy four-week-old mungbean plants (cv ‘Tainan No. 3’). Another three plants were not inoculated and were used as control. All the plants were maintained in a greenhouse at 25 to 28°C. All inoculated plants developed powdery mildew symptoms after 10 days, whereas the control plants remained symptomless. To our knowledge, this is the first report of P. xanthii causing disease on mungbean in Taiwan. P. xanthii also has been reported on mungbean in Thailand (Meeboon et al. 2016), while other records referring to E. polygoni infecting Vigna spp. are from Brazil and Fiji (Farr & Rossman 2020). Although both P. xanthii and E. polygoni have now been reported as causing powdery mildew on mungbean in Taiwan, which species predominates or is more important remains unclear. A comprehensive survey with accurate species identification is required to develop effective management of the disease, particularly for resistance breeding.

Genetic Diversity Analysis and Validation of Microsatellite Markers Linked with Tolerance to Powdery Mildew Disease in Mungbean [Vigna radiata (L.) Wilczek]

2021 ◽  
Author(s):  
B.S. Pavithra ◽  
Laxmipreeya Behera ◽  
K.C. Samal
Keyword(s):  
Powdery Mildew ◽  
Resistance Gene ◽  
Vigna Radiata ◽  
Powdery Mildew Resistance ◽  
Grain Legume ◽  
Phenotypic Traits ◽  
Powdery Mildew Resistance Gene ◽  
Mildew Resistance ◽  
A Genome ◽  
Powdery Mildew Disease

Background: Mungbean [Vigna radiata (L.) Wilczek] is a self-pollinated diploid grain legume (2n=2x=22) crop and has a genome size of 560 Mb. The present study was concentrate to portray the nature and extent of genotypic variation exists among mungbean collections for a range of traits of potential agronomic and adaptive interests. Many diseases affect mungbean, causes the major constraint in the increasing production among which Powdery mildew disease caused by Erisyphepolygoni is economically significant because it reduces photosynthetic activity and physiological changes which results in 20-40 per cent reduction in yield. Some gene-specific marker were analysed and found associated with powdery mildew resistance in mungbean genotypes.Methods: The present investigation was carried out to evaluate thirty-one mungbean genotypes (including four checks) collected from the different parts of India. The genotypes were sown in an incomplete augmented bock design along with four checks varieties. viz. ‘Kamdev’, ‘OBBGG-52’, ‘IPM-02-14’ and ‘IPM-02-3’. All the mungbean genotypes were evaluated for different phenotypic traits and their tolerance to powdery mildew disease at two cropping seasons as well as at two different locations in Odisha. In this investigation, seven molecular markers viz., VrCSSTS1, VrCSSTS2, VrCSSSR3, CEDG191, MB-SSR238, CEDG166, CEDG282 were analysed.Result: SSR marker such as VrCSSSR and VrCSSTS linked with powdery mildew resistance gene were tested in different genotypes with known powdery mildew reaction and the results showed a consistent association of the marker in all the powdery mildew resistant genotypes and absent in all the powdery mildew susceptible genotypes. The results confirmed the validation of these markers with the powdery mildew resistance gene in different genetic backgrounds. Similarly, CEDG191, CEDG166, CEDG282 markers, reported to be linked to powdery mildew resistance, amplified the respective marker fragment of 100 to 300 bp in mungbean genotypes and were polymorphic. The above PCR-based and locus-specific markers could be employed for marker-assisted breeding (MAB) program as well as genotype conservation. These linked markers will boost the efficiency and precision of powdery mildew resistance breeding in mungbean.

Candidate gene mapping reveals VrMLO12 (MLO Clade II) is associated with powdery mildew resistance in mungbean (Vigna radiata [L.] Wilczek)

Plant Science ◽  
2020 ◽  
Vol 298 ◽  
pp. 110594
Author(s):  
Chutintorn Yundaeng ◽  
Prakit Somta ◽  
Jingbin Chen ◽  
Xingxing Yuan ◽  
Sompong Chankaew ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Gene Mapping ◽  
Candidate Gene ◽  
Vigna Radiata ◽  
Powdery Mildew Resistance ◽  
Mildew Resistance ◽  
Candidate Gene Mapping

scholarly journals Eco-Friendly Management of Powdery Mildew of Green Gram (Vigna radiata L.)

2017 ◽  
Vol 6 (7) ◽  
pp. 435-439 ◽  
Author(s):  
Rajesh Kumar Yadav ◽  
S.S. Kakraliya ◽  
M.R. Bajiya ◽  
Sonali Abrol
Keyword(s):  
Powdery Mildew ◽  
Vigna Radiata ◽  
Green Gram
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