Role of trace element chelation in the fusarium wilt of cotton

Author(s):  
D. Subramanian
Keyword(s):  
2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chunzhen Cheng ◽  
Fan Liu ◽  
Na Tian ◽  
Raphael Anue Mensah ◽  
Xueli Sun ◽  
...  

AbstractFusarium wilt disease, caused by Fusarium oxysporum f.sp. cubense (Foc), has been recognized as the most devastating disease to banana. The regulatory role of long non-coding RNAs (lncRNAs) in plant defense has been verified in many plant species. However, the understanding of their role during early FocTR4 (Foc tropical race 4) infection stage is very limited. In this study, lncRNA sequencing was used to reveal banana root transcriptome profile changes during early FocTR4 infection stages. Quantitative real time PCR (qRT-PCR) was performed to confirm the expression of eight differentially expressed (DE) lncRNAs (DELs) and their predicted target genes (DETs), and three DE genes (DEGs). Totally, 12,109 lncRNAs, 36,519 mRNAs and 2642 novel genes were obtained, of which 1398 (including 78 DELs, 1220 DE known genes and 100 DE novel genes) were identified as FocTR4 responsive DE transcripts. Gene function analysis revealed that most DEGs were involved in biosynthesis of secondary metabolites, plant–pathogen interaction, plant hormone signal transduction, phenylalanine metabolism, phenylpropanoid biosynthesis, alpha-linolenic acid metabolism and so on. Coincidently, many DETs have been identified as DEGs in previous transcriptome studies. Moreover, many DETs were found to be involved in ribosome, oxidative phosphorylation, lipoic acid metabolism, ubiquitin mediated proteolysis, N-glycan biosynthesis, protein processing in endoplasmic reticulum and DNA damage response pathways. QRT-PCR result showed the expression patterns of the selected transcripts were mostly consistent with our lncRNA sequencing data. Our present study showed the regulatory role of lncRNAs on known biotic and abiotic stress responsive genes and some new-found FocTR4 responsive genes, which can provide new insights into FocTR4-induced changes in the banana root transcriptome during the early pathogen infection stage.


Author(s):  
Anne-Aziliz Pelleter ◽  
Gaëlle Prouteau ◽  
Bruno Scaillet

Abstract We performed phase equilibrium experiments on a natural Ca-poor pelite at 3 GPa, 750-1000 °C, under moderately oxidizing conditions, simulating the partial melting of such lithologies in subduction zones. Experiments investigated the effect of sulphur addition on phase equilibria and compositions, with S contents of up to ∼ 2.2 wt. %. Run products were characterized for their major and trace element contents, in order to shed light on the role of sulphur on the trace element patterns of melts produced by partial melting of oceanic Ca-poor sediments. Results show that sulphur addition leads to the replacement of phengite by biotite along with the progressive consumption of garnet, which is replaced by an orthopyroxene-kyanite assemblage at the highest sulphur content investigated. All Fe-Mg silicate phases produced with sulphur, including melt, have higher MgO/(MgO+FeO) ratios (relative to S-free/poor conditions), owing to Fe being primarily locked up by sulphide in the investigated redox range. Secular infiltration of the mantle wedge by such MgO and K2O-rich melts may have contributed to the Mg and K-rich character of the modern continental crust. Addition of sulphur does not affect significantly the stability of the main accessory phases controlling the behaviour of trace elements (monazite, rutile and zircon), although our results suggest that monazite solubility is sensitive to S content at the conditions investigated. The low temperature (∼ 800 °C) S-bearing and Ca-poor sediment sourced slab melts show Th and La abundances, Th/La systematics and HFSE signatures in agreement with the characteristics of sediment-rich arc magmas. Because high S contents diminish phengite and garnet stabilities, S-rich and Ca-poor sediment sourced slab melts have higher contents of Rb, B, Li (to a lesser extent), and HREE. The highest ratios of La/Yb are observed in sulphur-poor runs (with a high proportion of garnet, which retains HREE) and beyond the monazite out curve (which retains LREE). Sulphides appear to be relatively Pb-poor and impart high Pb/Ce ratio to coexisting melts, even at high S content. Overall, our results show that Phanerozoic arc magmas from high sediment flux margins owe their geochemical signature to the subduction of terrigenous, sometimes S-rich, sediments. In contrast, subduction of such lithologies during Archean appears unlikely or unrecorded.


AgriPeat ◽  
2019 ◽  
Vol 20 (01) ◽  
pp. 19-26
Author(s):  
Admin Journal

ABSTRACTThe role of Trichocompost and KCl fertilizer to control Fusarium wilt disease on onion in sandy soil. Fusarium wilt on onion is an interesting disease it is can loss the onion yield. The purpose of research to study trichocompost and KCl fertilizer role to control Fusarium wilt disease on ann onion. The research design used a Factorial Randomized Block Design with 2 factors. The first factor is 4 levels trichocompost, it is: without trichocompost (T0), trichocompost 10 t.ha-1 dosage (T1), trichocompost 20 t.ha-1 dosage (T2), trichocompost 30 t.ha-1 dosage (T3). The second factor is 3 levels KCl fertilizer, it is: without KCl (K0), KCl 100 kg.ha-1 dosage (K1), KCl 200 kg.ha-1 dosage (K2). Result of this research showed the application of trichocompost 10 t.ha-1 dosage and KCl 100 kg.ha-1 dosage can inhibit Fusarium wilt incubation time, can inhibit the patogen development with effective value 89,23%, the single factor it is aplication trichocompost 10 t.ha-1 dosage and trichocompost 30 t.ha-1 dosage not significant to dried onion bulb weight per clump of onion plant.Key words: Trichocompost, KCl fertilizer, Fusarium wilt disease, onion, sandy soil.ABSTRAKPenyakit layu Fusarium merupakan salah satu penyakit penting dapat menurunkan produksi bawang merah hingga 50%. Tujuan penelitian untuk mengetahui peranan trichokompos dan pupuk KCl dalam mengendalikan penyakit layu fusarium pada tanaman bawang merah. Penelitian menggunakan Rancangan Acak Kelompok faktorial dua faktor perlakuan. Faktor pertama 4 taraf dosis trichokompos yaitu: tanpa trichokompos (T0), trichokompos dosis 10 t.ha-1 (T1), trichokompos dosis 20 t.ha-1 (T2), trichokompos dosis 30 t.ha-1 (T3). Faktor kedua 3 taraf dosis pupuk KCl yaitu: tanpa pupuk KCl (K0), pupuk KCl dosis 100 KCl kg.ha-1 (K1), pupuk KCl dosis 200 KCl kg.ha-1 (K2). Hasil penelitian menunjukkan pemberian trichokompos 10 t.ha-1 dan pupuk KCl 100 kg.ha-1 dapat memperpanjang masa inkubasi penyakit, menekan serangan penyakit layu Fusarium dengan nilai efektivitas sangat baik (89,23%), perlakuan tunggal trichokompos dosis 10 t.ha-1 tidak berbeda nyata dengan dosis 30 t.ha-1 terhadap bobot umbi kering per rumpun tanaman bawang merah.Kata kunci: penyakit layu Fusarium, pupuk KCl, tanah berpasir, tanaman bawang merah, trichokompos.


2018 ◽  
Vol 24 (6) ◽  
pp. 604-611 ◽  
Author(s):  
Ben Buse ◽  
Jon Wade ◽  
Xavier Llovet ◽  
Stuart Kearns ◽  
John J. Donovan

AbstractSecondary fluorescence (SF), typically a minor error in routine electron probe microanalysis (EPMA), may not be negligible when performing high precision trace element analyses in multiphase samples. Other factors, notably wavelength dispersive spectrometer defocusing, may introduce analytical artifacts. To explore these issues, we measured EPMA transects across two material couples chosen for their high fluorescence yield. We measured transects away from the fluorescent phase, and at various orientations with respect to the spectrometer focal line. Compared to calculations using both the Monte Carlo simulation code PENEPMA and the semi-analytical model FANAL, both codes estimate the magnitude of SF, but accurate correction requires knowledge of the position of the spectrometer with respect to the couple interface. Positioned over the fluorescent phase or otherwise results in a factor of 1.2–1.8 of apparent change in SF yield. SF and spectrometer defocusing may introduce systematic errors into trace element analyses, both may be adequately accounted for by modeling. Of the two, however, SF is the dominant error, resulting in 0.1 wt% Zn apparently present in Al at 100 μm away from the Zn boundary in an Al/Zn couple. Of this, around 200 ppm Zn can be attributed to spectrometer defocusing.


2020 ◽  
Vol 17 (23) ◽  
pp. 5989-6015
Author(s):  
Quentin Charbonnier ◽  
Julien Bouchez ◽  
Jérôme Gaillardet ◽  
Éric Gayer

Abstract. The biological cycle of rock-derived nutrients on the continents is a major component of element transfer between the Earth's surface compartments, but its magnitude currently remains elusive. The use of the stable isotope composition of rock-derived nutrients, which can be fractionated during biological uptake, provides a promising path forward with respect to quantifying biological cycling and its overall contribution to global element cycling. In this paper, we rely on the nutrient-like behaviour of the trace element barium (Ba) and use its elemental and stable isotope compositions in dissolved and sediment load river samples to investigate biological cycling in the Amazon Basin. From these measurements, we show that dissolved Ba mainly derives from silicate rocks, and a correlation between dissolved Ba and K abundances suggests that biological cycling plays a role in the Ba river budget. Furthermore, the isotope composition of Ba (δ138Ba) in the dissolved load was found to be significantly different from that of the parent silicate rocks, implying that dissolved Ba isotopic signatures are affected by (i) the precipitation of soil-forming secondary phases as well as (ii) biological uptake and release from dead organic matter. Results from an isotope mass balance method applied to the river dissolved load data indicate that, after its release to solution by rock weathering, Ba is partitioned between the river dissolved load, secondary weathering products (such as those found in soils and river sediments), and the biota. In most sub-catchments of the Amazon, river Ba abundances and isotope compositions are significantly affected by biological cycling. Relationships between estimates of Ba cycled through biota and independent metrics of ecosystem dynamics (such as gross primary production and terrestrial ecosystem respiration) allow us to discuss the role of environmental parameters such as climate or erosion rates on the biological cycling of Ba and, by extension, the role of major rock-derived nutrients. In addition, catchment-scale mass and isotope budgets of Ba show that the measured riverine export of Ba is lower than the estimated delivery of Ba to the Earth surface through rock alteration. This indicates the existence of a missing Ba component, which we attribute to the formation of Ba-bearing particulate organics (possibly accumulating as soil organic matter or currently growing biomass within the catchments) and to organic-bound Ba exported as “unsampled” river particulate organic matter. Given our findings on the trace element Ba, we explore whether the river fluxes of most major rock-derived nutrients (K, Mg, Ca) might also be significantly affected by biological uptake or release. A first-order correction of river-derived silicate weathering fluxes from biological cycling shows that the carbon dioxide (CO2) consumption by silicate weathering at the mouth of the Amazon could be several times higher than the previously reported value of 13 × 109 mol CO2 yr−1 (Gaillardet et al., 1997). Overall, our study clearly shows that the chemical and isotope compositions of rivers in the Amazon – and most likely in other large river basins – bear a biological imprint, thereby challenging common assumptions made in weathering studies.


2019 ◽  
Vol 8 (4) ◽  
pp. 304-312
Author(s):  
Dahou Moutassem ◽  
Lakhdar Belabid ◽  
Yuva Bellik ◽  
Noureddine Rouag ◽  
Hanane Abed ◽  
...  

2019 ◽  
Vol 104 (8) ◽  
pp. 1180-1188 ◽  
Author(s):  
Shuo Yin ◽  
Richard Wirth ◽  
Changqian Ma ◽  
Jiannan Xu

Abstract The migrating fluid-mineral interface provides an opportunity for the uptake of trace elements as solid solutions in the newly formed crystal lattice during the non-equilibrium growth of the crystal. However, mineral nanoparticles could precipitate directly from the interfacial fluid when it evolves to a supersaturated situation. To better understand the role of mineral nanoparticles in this scenario, this study focuses on a well-documented magnetite with oscillatory zoning from a skarn deposit by using high-resolution transmission electron microscopy (TEM). Our results show that the Al concentration in magnetite measured on a micrometer-scale is caused by three different effects: Al solid solution, Al-rich nanometer-sized lamellae, and zinc spinel nanoparticles in the host magnetite. Here, we propose a genetic relationship among the three different phases mentioned above. At first, a continuous increase of the Al concentration in the interfacial fluid can be incorporated into the crystal lattice of magnetite forming a solid solution. During cooling in a later stage, aluminum in magnetite is oversaturated and exsolution of hercynite (Al-rich lamellae) occurs from the host magnetite. If the Al concentration at the fluid-magnetite interface still increases during further growth of magnetite, the substitution of Fe by Al has gradually reached saturation so that aluminum cannot be incorporated in the magnetite crystal structure any longer. Using the magnetite lattice as a template, nucleation of abundant zinc spinel nanoparticles occurs. This will, in turn, lead to a gradual depletion of Al concentration in the interfacial fluid until the available ions for zinc spinel nucleation and growth have been used up. As a result, the migrating fluid-magnetite interface will enrich the Al concentration in the interfacial fluid until the available ion concentration is sufficient for nucleation of zinc spinel phase again. The fluid-mineral interface in this mechanism has been repeatedly utilized during crystal growth, providing an efficient way for the uptake of trace element from a related undersaturated bulk fluid.


Sign in / Sign up

Export Citation Format

Share Document