Differential protection of photosynthetic capacity in trehalose-and lea protein-producing transgenic plants under abiotic stresses

2008 ◽  
Vol 51 (5) ◽  
pp. 327-336 ◽  
Author(s):  
Sung -Soo Jun ◽  
Hye Jin Choi ◽  
Hae Youn Lee ◽  
Young -Nam Hong
Keyword(s):  
Transgenic Plants ◽  
Abiotic Stresses ◽  
Photosynthetic Capacity ◽  
Differential Protection ◽  
Lea Protein

scholarly journals Expression of a luteoviral movement protein in transgenic plants leads to carbohydrate accumulation and reduced photosynthetic capacity in source leaves

1997 ◽  
Vol 12 (5) ◽  
pp. 1045-1056 ◽  
Author(s):  
Karin Herbers ◽  
Eckhardt Tacke ◽  
Mohammad Hazirezaei ◽  
Klaus-Peter Krause ◽  
Michael Melzer ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Photosynthetic Capacity ◽  
Movement Protein ◽  
Carbohydrate Accumulation

scholarly journals The soybean GmDi19-5 interacts with GmLEA3.1 and increases sensitivity of transgenic plants to abiotic stresses

2015 ◽  
Vol 6 ◽  
Author(s):  
Zhi-Juan Feng ◽  
Xiao-Yu Cui ◽  
Xi-Yan Cui ◽  
Ming Chen ◽  
Guang-Xiao Yang ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Abiotic Stresses

Transgenic plants tolerant to abiotic stresses

2009 ◽  
Vol 43 (2) ◽  
pp. 132-149 ◽  
Author(s):  
Ya. S. Kolodyazhnaya ◽  
N. K. Kutsokon ◽  
B. A. Levenko ◽  
O. S. Syutikova ◽  
D. B. Rakhmetov ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Abiotic Stresses

scholarly journals Analysis of LEA protein family members in Lepidium apetalum seeds and the expression of LaLEA1 in seedlings in response to abiotic stresses

2020 ◽  
Vol 64 ◽  
pp. 211-219 ◽  
Author(s):  
Q.L. YANG ◽  
H. LU ◽  
Q. ZHOU ◽  
H.T. XIE ◽  
J.Y. LI ◽  
...  
Keyword(s):  
Abiotic Stresses ◽  
Family Members ◽  
Protein Family ◽  
Lea Protein

scholarly journals Overexpression of A Biotic Stress-Inducible Pvgstu Gene Activates Early Protective Responses in Tobacco under Combined Heat and Drought

2021 ◽  
Vol 22 (5) ◽  
pp. 2352
Author(s):  
Evangelia Stavridou ◽  
Georgia Voulgari ◽  
Michail Michailidis ◽  
Stefanos Kostas ◽  
Evangelia G. Chronopoulou ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Abiotic Stresses ◽  
Differentially Expressed ◽  
Plant Performance ◽  
Primary Metabolism ◽  
Combined Stress ◽  
Protective Mechanisms ◽  
Leading Role ◽  
Wide Range ◽  
Major Factors

Drought and heat stresses are major factors limiting crop growth and productivity, and their effect is more devastating when occurring concurrently. Plant glutathione transferases (GSTs) are differentially expressed in response to different stimuli, conferring tolerance to a wide range of abiotic stresses. GSTs from drought-tolerant Phaseolus vulgaris var. “Plake Megalosperma Prespon” is expected to play an important role in the response mechanisms to combined and single heat and drought stresses. Herein, we examined wild-type N. tabacum plants (cv. Basmas Xanthi) and T1 transgenic lines overexpressing the stress-induced Pvgstu3–3 and Pvgstu2–2 genes. The overexpression of Pvgstu3–3 contributed to potential thermotolerance and greater plant performance under combined stress. Significant alterations in the primary metabolism were observed in the transgenic plants between combined stress and stress-free conditions. Stress-responsive differentially expressed genes (DEGs) and transcription factors (TFs) related to photosynthesis, signal transduction, starch and sucrose metabolism, osmotic adjustment and thermotolerance, were identified under combined stress. In contrast, induction of certain DEGs and TF families under stress-free conditions indicated that transgenic plants were in a primed state. The overexpression of the Pvgstu3–3 is playing a leading role in the production of signaling molecules, induction of specific metabolites and activation of the protective mechanisms for enhanced protection against combined abiotic stresses in tobacco.

scholarly journals Transient expression of gene encoding ZmLEA14A protein in Nicotiana benthamiana plant

2020 ◽  
Vol 17 (3) ◽  
pp. 491-497
Author(s):  
Ha Hong Hanh ◽  
Le Thi Thu Hien ◽  
Huynh Thi Thu Hue
Keyword(s):  
Genetic Engineering ◽  
Abiotic Stresses ◽  
Nicotiana Benthamiana ◽  
Lea Proteins ◽  
Lea Protein ◽  
Gene Encoding ◽  
Specific Antibodies ◽  
Vegetative Tissues ◽  
The World ◽  
Infiltration Method

LEA protein family includes proteins accumulated in the late stage of embryogenesis and in vegetative tissues of stress-confronted plant. These proteins have been demontrated to play a major role in plant response to abiotic stresses, such as drought and salinity stress. The genes coding for LEA proteins in maize are divided into 9 groups including LEA 1, LEA 2, LEA 3, LEA 4, LEA 5, LEA 6, SMP, dehydrin, and AtM. The application of LEA genes to improve drought tolerance for plants by genetic engineering has also been studied extensively all over the world. In this study, pCAM/35S-ZmLEA14A-35S vector and pCAM/Ubi-ZmLEA14A-35S vector contained the ZmLEA14A gene isolated from Te vang 1, these vectors were used to transient express into Nicotiana benthamiana tobacco leaves by agro-infiltration method. The results of immunoassay between cmyc specific antibodies with proteins from infected leaves revealed the expression of recombinant ZmLEA14A protein in N. benthamiana leaves. Thereby, two constructs habouring the ZmLEA14A gene work at transcription and translation levels in the model plant that could harnessed for stable transformation in plants.

scholarly journals Assessment of antimicrobial phytopeptides: lipid transfer protein and hevein-like peptide in the prospect of structure, function and allergenic effect

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Sarfuddin Azmi ◽  
Shahnaaz Khatoon ◽  
Mohd Kamil Hussain
Keyword(s):  
Transgenic Plants ◽  
Antimicrobial Peptides ◽  
Abiotic Stresses ◽  
Lipid Transfer Protein ◽  
Allergic Sensitization ◽  
Food Allergies ◽  
Main Body ◽  
Lipid Transfer ◽  
Transfer Protein ◽  
Wide Range

Abstract Background Antimicrobial peptides (AMPs) are unique natural antibiotics that are crucial effectors of innate immune systems in almost all living organisms. Several different plant antimicrobial peptides have been identified and isolated, demonstrating a high level of protection against various types of bacteria, insects, nematodes and other microbes. Along with antimicrobial function, these peptides play a wide range of crucial function in plants, such as regulation of stomata, ion channel, heavy metals and membrane fluidity. Main body Antimicrobial peptides show a continuum of toxicity for a variety of plants and animals pathogenic microbes and even show cytotoxicity against cancer cells. Numerous studies have shown that transgenic plants have increased the expression of AMP-encoding genes in response to biotic and abiotic stresses, and plants that express transgenic AMP genes are more responsive to biotic, abiotic and other functions. In addition to being a molecule with protective properties, various allergic reactions are associated with some phytopeptides and proteins, in particular non-specific lipid transfer protein (nsLTP) and peptide-like hevein. Pru p3 from peach is the most clinically important allergen within the nsLTP family that cause real food allergies and also triggers extreme clinical reactions. Similarly, latex-fruit syndrome was primarily associated with well-studied latex allergen Hevein (Hev b8, Hev b6) and class I chitinases. Short conclusions Several findings have shown that, in the near future, transgenic plants based on AMPs against the verity of pathogenic fungi, bacteria and other abiotic stresses will be released without any adverse effects. Recent study reason that association of lipid with nsLTP enhances allergic sensitization and hevein-like domain of chitinase I essentially plays a role in cross-sensitivity of latex with different fruits and nuts. This review discusses the structures and various functions of lipid transfer protein and hevein-like peptide.

scholarly journals GENETIC MODIFICATION FOR SALT AND DROUGHT TOLERANCE IN PLANTS THROUGH SODERF3

2020 ◽  
Vol 2020 (1) ◽  
Author(s):  
M Ali ◽  
F Rafique ◽  
Q Ali ◽  
A Malik
Keyword(s):  
Transgenic Plants ◽  
Drought Tolerance ◽  
Genetic Modification ◽  
Salt Concentration ◽  
Abiotic Stresses ◽  
Major Part ◽  
High Salt Concentration ◽  
Biotic And Abiotic Stresses ◽  
Ethylene Responsive Factor ◽  
The Stability

Plants constitute the major part of the ecosystem and maintain balance through their different roles in the stability of the environment. As plants have an impact over environment; in the same manner environment interacts with plants. These interactions bring some productive results or sometimes may cause serious issues to plants. The environment poses some serious threats to plants as it is changing drastically over the course of years. Plants have been resistant to many of biotic and abiotic stresses naturally but now it is getting challenging. The major issues faced by plants are drought, high salt concentration, temperature and many other factors. These issues can be compensated by engineering plants with such novel genes which cause the release of ethylene responsive factor in the case of drought and salt intolerance. There are various studies to engineer the stress sensitive plants with SodERF3, a novel sugarcane ethylene responsive factor which causes promising tolerance in transgenic plants.

scholarly journals Αξιοποίηση μοριακών μεθόδων στη βελτίωση ανθεκτικότητας των εσπεριδοειδών στο Citrus exocortis viroid, στον έλεγχο της υγείας των εσπεριδοειδών υπό εξυγίανση και στη μελέτη διαφορικής έκφρασης γονιδίων ποικιλιών λεμονιάς

2016 ◽  
Author(s):  
Ευαγγελία Κουτσιουμάρη
Keyword(s):  
Transgenic Plants ◽  
Genetic Engineering ◽  
Abiotic Stresses ◽  
Sour Orange ◽  
Wild Type ◽  
Citrus Species ◽  
Biotic And Abiotic Stresses ◽  
Carrizo Citrange ◽  
The Difference

Citrus, commercially grown in a wide range of soil and climatic conditions, aresubjected to substantial biotic and abiotic stresses which limit the production and insome cases, pose restrictions on the use of specific rootstocks and varieties. Citrusexocortis viroid (CEVd) causes severe symptoms in trees grafted on Poncirus trifoliata(L.) Raf. and its hybrids which, due to their tolerance to Citrus tristeza virus, arerecently employed for the replacement of sour orange (Citrus aurantium L.). The mosteffective method of controlling both biotic and abiotic stresses in plants refers to the useof resistant varieties. Nevertheless, the lack of natural genetic sources of resistance tomost severe diseases, along with the lack of basic knowledge on the inheritance patternof main agronomic traits, render necessary the use of genetic engineering in citrusbreeding. To this direction, the exploitation of genetic engineering requires theexistence of an efficient tissue culture protocol for each citrus species, to ensureregeneration of sufficient number of transformed plants. In this framework, the presentstudy initially focused on the determination of the most suitable genetic transformationprotocol for each citrus species: P. trifoliata, Carrizo citrange (C. sinensis × P.trifoliata), Citrumelo 1452 (P. trifoliata × C. paradisi), sour orange and “Maglini”lemon (Citrus limon (L.) Burm. f.) (Chapter 1).In recent years, one of the most efficient methods for the generation of resistanceagainst viruses and viroids refers to the exploitation of RNA silencing (RNAi). Towardsthis direction, and in view of the recalcitrant nature of citrus species which renderstransformation and regeneration particularly difficult, aim of this study was thedevelopment of transgenic CEVd resistance in the model plant Nicotiana benthamianawhich is a non-host of CEVd. In this line, the CEVd-inoculation of N. benthamianaplants was pursued by two different approaches: a) agroinfiltration and b) stable genetictransformation, using a plasmid harboring the dimeric CEVd molecule (Chapter 2). Theresults indicate a low rate of CEVd replication in the agro-infiltrated plants, in contrastto transgenic plants which were capable of CEVd replication. The latter though, werecharacterized by reduced growth and seed production compared to wild-type plants. Towards investigating the capability of various segments of the CEVd genome ininducing silencing of its genome and subsequent suppression of its replication,transgenic plants replicating the viroid were inoculated with two different selfcomplementaryhairpin RNA fragments from the CEVd genome (Chapter 3). Theresults provided strong evidence that both CEVd regions are capable of triggering RNAsilencing, thus causing a reduction to the viroid replication rate. These results were alsoconfirmed by transient expression experiments in wild-type N. benthamiana plants,where viroid presence caused reduced accumulation of the selected CEVd fragments.As a means to investigate whether the observed resistant phenotype of the modelplant N. benthamiana can be achieved in citrus species, which consist the natural viroidhosts, the introgression of two selected segments of the CEVd genome was pursued, bygenetic transformation, in various citrus genotypes (Chapter 4). To this purpose, genetictransformation was performed in plants of P. trifoliata, Carrizo citrange and Citrumelo1452. Parallel aim was the generation of transgenic resistance against citrus psorosisdisease, through the introgression of the viral coat protein of Citrus psorosis virus(CPsV) in sour orange and “Maglini” lemon (Chapter 4).The most important method to control citrus diseases is the use of healthy certifiedpropagation material. An effective method for plant sanitation is in vitro micrograftingof apical meristems. The study of in vitro micrografted lemon, orange and mandarinplants verified the absence of the most important viruses and viroids for Greekcitriculture, indicating that the necessary expertise for sanitation of the precious citruspropagation material exists in our country (Chapter 5).Finally, this dissertation included the study of certain sequences which areoverexpressed in the Greek lemon ”Adamopoulou” compared to the Portuguese”Lisbon” (Chapter 6). The difference between the two varieties is that ”Adamopoulou”is tolerant to mal secco and cold compared to “Lisbon”. Several of the sequences understudy presented homology with proteins directly or indirectly involved in the defensemechanisms of plants against biotic and abiotic stresses, with the difference between thetwo varieties in expression of seven genes being at varying levels.

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