Gene expression of chloroplast translation elongation factor Tu during maize chloroplast biogenesis

1997 ◽  
Vol 40 (4) ◽  
pp. 227-233 ◽  
Author(s):  
Jeong Hwan Lee ◽  
Il Ho Kang ◽  
Kyu Lee Choi ◽  
Woong-Seop Sim ◽  
Jeong-Kook Kim
Keyword(s):  
Gene Expression ◽  
Elongation Factor ◽  
Elongation Factor Tu ◽  
Chloroplast Biogenesis ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Chloroplast Translation ◽  
Maize Chloroplast

scholarly journals Reference Gene Selection for Quantitative Real-Time RT-PCR Normalization inIris. lacteavar.chinensisRoots under Cadmium, Lead, and Salt Stress Conditions

2014 ◽  
Vol 2014 ◽  
pp. 1-7 ◽  
Author(s):  
Chun-Sun Gu ◽  
Liang-qin Liu ◽  
Chen Xu ◽  
Yan-hai Zhao ◽  
Xu-dong Zhu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Salt Stress ◽  
Real Time ◽  
Reference Genes ◽  
Elongation Factor ◽  
Stress Conditions ◽  
Translation Initiation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Wide Range

Quantitative real time PCR (RT-qPCR) has emerged as an accurate and sensitive method to measure the gene expression. However, obtaining reliable result depends on the selection of reference genes which normalize differences among samples. In this study, we assessed the expression stability of seven reference genes, namely, ubiquitin-protein ligase UBC9 (UBC), tubulin alpha-5 (TUBLIN), eukaryotic translation initiation factor (EIF-5A), translation elongation factor EF1A (EF1α), translation elongation factor EF1B (EF1b), actin11 (ACTIN), and histone H3 (HIS), inIris. lacteavar.chinensis(I. lacteavar.chinensis) root when the plants were subjected to cadmium (Cd), lead (Pb), and salt stress conditions. All seven reference genes showed a relatively wide range of threshold cycles (Ct) values in different samples. GeNorm and NormFinder algorithms were used to assess the suitable reference genes. The results from the two software units showed thatEIF-5AandUBCwere the most stable reference genes across all of the tested samples, whileTUBLINwas unsuitable as internal controls.I. lacteavar.chinensisis tolerant to Cd, Pb, and salt. Our results will benefit future research on gene expression in response to the three abiotic stresses.

A pea chloroplast translation elongation factor that is regulated by abiotic factors

2004 ◽  
Vol 320 (2) ◽  
pp. 523-530 ◽  
Author(s):  
B.N Singh ◽  
R.N Mishra ◽  
Pradeep K Agarwal ◽  
Mamta Goswami ◽  
Suresh Nair ◽  
...  
Keyword(s):  
Abiotic Factors ◽  
Elongation Factor ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Chloroplast Translation

scholarly journals Structural dynamics of translation elongation factor Tu during aa-tRNA delivery to the ribosome

2018 ◽  
Vol 46 (16) ◽  
pp. 8651-8661 ◽  
Author(s):  
Darius Kavaliauskas ◽  
Chunlai Chen ◽  
Wei Liu ◽  
Barry S Cooperman ◽  
Yale E Goldman ◽  
...  
Keyword(s):  
Structural Dynamics ◽  
Elongation Factor ◽  
Elongation Factor Tu ◽  
Translation Elongation Factor ◽  
Translation Elongation
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