Micropropagation ofDendrobium fimbriatum Hook. by green pod culture

2005 ◽  
Vol 48 (2) ◽  
pp. 253-257 ◽  
Author(s):  
Ramnath Sharma ◽  
K. K. De ◽  
Bibaychana Sharma ◽  
Sudipta Majumdar
Keyword(s):  
Pod Culture

scholarly journals Green pod culture and rapid micropropagation of Dendrobium chrysanthum Wall. – A horticultural and medicinal orchid

2013 ◽  
Vol 25 (2) ◽  
pp. 169
Author(s):  
Kalyan Kumar De ◽  
Sudipta Majumdar ◽  
Ramnath Sharma ◽  
Bibaychana Sharma
Keyword(s):  
Medicinal Orchid ◽  
Dendrobium Chrysanthum ◽  
Pod Culture

scholarly journals Seed and Embryo Growth in Pod Cultures of Phaseolus vulgaris and P. vulgaris × P. acutifolius

HortScience ◽  
1990 ◽  
Vol 25 (10) ◽  
pp. 1288-1291 ◽  
Author(s):  
Ana Maria Sabja ◽  
David W.S. Mok ◽  
Machteld C. Mok
Keyword(s):  
Interspecific Hybrid ◽  
Embryo Rescue ◽  
Precocious Germination ◽  
Developmental Arrest ◽  
Hybrid Embryo ◽  
Murashige Skoog ◽  
Cotyledonary Stage ◽  
Pod Culture ◽  
Culture Procedure

Interspecific hybrid embryos resulting from crosses between Phaseolus species generally fail to reach maturity, and embryo rescue techniques are required to recover plants. To determine if ovary (pod) culture could permit interspecific hybrid embryo development, pods of P. vulgaris L. × P. acutifolius A. Gray and P. vulgaris L. (control) were cultured upright, supported by glasswool, in modified Murashige-Skoog liquid medium. The weight of seeds and length of embryos in P. vulgaris pods increased significantly during culture. However, usually only one or occasionally two seeds located at the middle positions of the pods developed to maturity. The same pod culture procedure promoted precocious germination of early cotyledonary-stage P. vulgaris × P. acutifolius embryos without further maturation of the embryos. These findings confirm that developmental arrest of the interspecific hybrid embryos in vivo is due to intrinsic deficiencies.

scholarly journals Morphophysiology and Cryopreservation of Seeds of Dendrobium Nobile Lindl. (Orchidaceae) at Different Stages of Development

2021 ◽  
Author(s):  
Galina L Kolomeitseva ◽  
Tatiana V Nikishina ◽  
Alexander V Babosha ◽  
Andrey S Ryabchenko ◽  
Olga N Vysotskaya
Keyword(s):  
Seed Viability ◽  
Embryo Sac ◽  
Dendrobium Nobile ◽  
Long Term Storage ◽  
Immature Seeds ◽  
Dendrobium Nobile Lindl ◽  
Term Storage ◽  
Pod Culture

Abstract We studied the seed maturation of Dendrobium nobile Lindl to establish the optimal stage for green pod culture and long-term storage in liquid nitrogen. It was found that the immature seeds can germinate in vitro starting from 3 months after pollination (MAP) but only develop up to the stage of embryo swelling or protocorm without rhizoids. The maximum staining of embryos with vital dyes FDA and TTC occurs at 4 and 5 MAP that corresponds to the release of suspensor beyond the embryo sac. Embryo staining does not correlate with germination capacity and seed viability in cryogenic storage. The immature seeds acquire resistance to freezing and drying in airflow at the age of 6 MAP. The time of technical maturity for green pod culture and cryopreservation may be reduced from 12 MAP to 6–7 MAP.

Development of an In vitro pod culture technique for young pods of Phaseolus vulgaris L.

2000 ◽  
Vol 36 (6) ◽  
pp. 481-487 ◽  
Author(s):  
Pascal Geerts ◽  
Khaled Sassi ◽  
Guy Mergeai ◽  
Jean-Pierre Baudoin
Keyword(s):  
Phaseolus Vulgaris ◽  
Culture Technique ◽  
Phaseolus Vulgaris L ◽  
Pod Culture

Soya Bean Seed Growth and Maturation by In Vitro Pod Culture

1983 ◽  
Vol 51 (2) ◽  
pp. 217-227 ◽  
Author(s):  
R. L. OBENDORF ◽  
G. T. RYTKO ◽  
M. C. BYRNE
Keyword(s):  
Soya Bean ◽  
Bean Seed ◽  
Seed Growth ◽  
Pod Culture

In vitro pod culture of Pisum sativum

Plant Science ◽  
1986 ◽  
Vol 43 (3) ◽  
pp. 223-228 ◽  
Author(s):  
D.H.P. Barratt
Keyword(s):  
Pisum Sativum ◽  
Pod Culture
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