β-Glucan synthetase II activity upon callose formation in the flower ofArabidopsis thaliana

1998 ◽  
Vol 41 (2) ◽  
pp. 110-115
Author(s):  
Joungyoun Kang ◽  
Youngsoon Kim ◽  
Hyeonsook Cheong ◽  
Byoungsik Pyo ◽  
Baik Hwang
Keyword(s):  
Ofarabidopsis Thaliana ◽  
Callose Formation ◽  
Glucan Synthetase

SEM and fluorescence imaging of callose deposition in root hair tips and suspension cultures of Streptanthus tortuosus

1990 ◽  
Vol 48 (3) ◽  
pp. 698-699
Author(s):  
K.S. Walters ◽  
R.D. Sjolund ◽  
K.C. Moore
Keyword(s):  
Cell Wall ◽  
Root Hair ◽  
Cultured Cells ◽  
Root Hairs ◽  
Callus Cultures ◽  
Callose Deposition ◽  
Culture Cells ◽  
Wall Structures ◽  
Ultraviolet Illumination ◽  
Callose Formation

Callose, B-1,3-glucan, a component of cell walls, is associated with phloem sieve plates, plasmodesmata, and other cell wall structures that are formed in response to wounding or infection. Callose reacts with aniline blue to form a fluorescent complex that can be recognized in the light microscope with ultraviolet illumination. We have identified callose in cell wall protuberances that are formed spontaneously in suspension-cultured cells of S. tortuosus and in the tips of root hairs formed in sterile callus cultures of S. tortuosus. Callose deposits in root hairs are restricted to root hair tips which appear to be damaged or deformed, while normal root hair tips lack callose deposits. The callose deposits found in suspension culture cells are restricted to regions where unusual outgrowths or protuberances are formed on the cell surfaces, specifically regions that are the sites of new cell wall formation.Callose formation has been shown to be regulated by intracellular calcium levels.

N form–dependent growth retardation ofArabidopsis thaliana seedlings as revealed from physiological and microarray studies

2007 ◽  
Vol 170 (1) ◽  
pp. 87-97 ◽  
Author(s):  
Anne Hoffmann ◽  
Sabine Milde ◽  
Christine Desel ◽  
Anja Hümpel ◽  
Hartmut Kaiser ◽  
...  
Keyword(s):  
Growth Retardation ◽  
Ofarabidopsis Thaliana ◽  
N Form ◽  
Dependent Growth ◽  
Microarray Studies

scholarly journals Correction to Metabolic Profiling of Root Exudates ofArabidopsis thaliana

2009 ◽  
Vol 57 (19) ◽  
pp. 9346-9346 ◽  
Author(s):  
Travis S. Walker ◽  
Harsh Pal Bais ◽  
Kathleen M. Halligan ◽  
Frank R. Stermitz ◽  
Jorge M. Vivanco
Keyword(s):  
Root Exudates ◽  
Metabolic Profiling ◽  
Ofarabidopsis Thaliana

Purification par séparation de phase et caractérisation du plasmalemme de l'épicotyle de pois

1986 ◽  
Vol 64 (5) ◽  
pp. 448-455 ◽  
Author(s):  
Jacques Rembur ◽  
Pierre Landré ◽  
Arlette Nougarède
Keyword(s):  
Atpase Activity ◽  
Plasma Membranes ◽  
Microsomal Fraction ◽  
Alkaline Ph ◽  
Phase Partition ◽  
Pea Epicotyl ◽  
Glucan Synthetase ◽  
Triton X ◽  
Two Sides ◽  
Slight Contamination

The validity of phase partition to obtain a substantial proportion of vesicles of plasmalemma origin from the microsomal fraction of pea epicotyl has been demonstrated. In the fractions enriched with plasma membranes, N-naphthyl phtalamic acid binding and β-glucan synthetase II activity, showed a yield of about 60% and an enrichment of 2.3 and 2.2, respectively, in comparison with the microsomal fraction. When such plasmalemmic vesicles are permabilized by Triton X-100, an intense Mg2+-ATPase activity is obtained in presence of K+ at acid as well as alkaline pH. Inhibition of Mg2+-ATPase by vanadate in presence of K+ and its variations in relation to pH were shown. Dicyclohexylcarbodiimide and diethylstilbestrol inhibit 40–55% of this enzymatic activity, both at acid and neutral pH. The data show a slight contamination of the plasmalemmic fraction by endomembranes and suggest an asymmetry of the two sides of the plasmalemma.

scholarly journals Deletion of a Histidine-rich Loop of AtMTP1, a Vacuolar Zn2+/H+Antiporter ofArabidopsis thaliana, Stimulates the Transport Activity

2008 ◽  
Vol 283 (13) ◽  
pp. 8374-8383 ◽  
Author(s):  
Miki Kawachi ◽  
Yoshihiro Kobae ◽  
Tetsuro Mimura ◽  
Masayoshi Maeshima
Keyword(s):  
Transport Activity ◽  
Ofarabidopsis Thaliana
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