Cadmium-induced changes in antioxidant enzyme activities in rice (Oryza sativa L. cv. Dongjin)

2002 ◽  
Vol 45 (3) ◽  
pp. 134-140 ◽  
Author(s):  
Mohammad Babar Ali ◽  
Hyun Sik Chun ◽  
Byung Ki Kim ◽  
Chin Bum Lee
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Induced Changes

scholarly journals Seed priming with hydrogen peroxide alleviates the effects of drought stress in rice (Oryza sativa L.) seedlings

2020 ◽  
Vol 48 (1) ◽  
pp. 273-283
Author(s):  
Weeraphorn JIRA-ANUNKUL ◽  
Wattana PATTANAGUL
Keyword(s):  
Hydrogen Peroxide ◽  
Oryza Sativa ◽  
Drought Stress ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Oryza Sativa L ◽  
Seed Priming ◽  
Rice Seedling ◽  
Antioxidant Enzyme Activities ◽  
Guaiacol Peroxidase

Drought stress is a major factor limiting crop growth and yield. Hydrogen peroxide (H2O2) is known as a signalling molecule in the plant cell in which activates multiple physiological changes that play essential roles in tolerance mechanism. This study investigated the effects of seed priming with H2O2 on growth, some physiological characteristics and antioxidant enzyme activities in rice seedling under drought stress. Rice (Oryza sativa L.) cv. Khao Dawk Mali 105 seeds were primed with 0 (distilled water), 1, 5, 10, and 15 mM H2O2 and grown for 21 days. The seedlings were subjected to drought stress by withholding water for 7 days. The results showed that priming with low concentrations of H2O2 improved plant growth and biomass as well as relative water content, malondialdehyde content, electrolyte leakage. Priming with H2O2, however, had no beneficial effect on chlorophyll content, proline and leaf total soluble sugar. Seed priming with appropriate levels of H2O2 also enhanced antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and guaiacol peroxidase (GPX). It is concluded that seed priming with 2-10 mM H2O2, is beneficial for enhancing drought tolerance in rice seedling by increasing antioxidant capacity, which in turn reduces oxidative stress and damages to the cellular components.

The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings

2007 ◽  
Vol 87 (1) ◽  
pp. 49-57 ◽  
Author(s):  
J. Chen ◽  
C. Zhu ◽  
D. Lin ◽  
Z. -X Sun
Keyword(s):  
Lipid Peroxidation ◽  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Antioxidant Enzyme Activities ◽  
Wild Type ◽  
Rice Seedlings ◽  
Sensitive Mutant ◽  
Cd Toxicity ◽  
Potential Biomarker

Cadmium-sensitive rice (Oryza sativa L. subsp. Japonica ‘Zhonghua11’) mutants were obtained using an Agrobacterium tumefaciens-based gene delivery system. Significant phenotypic differences were observed between a Cd-sensitive mutant (ST) and wild type (WT) rice seedlings. Results indicated that Cd accumulation in the leaves of the mutant was twice that of the wild type after 10 d of 0.5 mM Cd2+ treatment. Furthermore, a rapid Cd-induced H2O2 increase was observed in the mutant leaves, which induced abnormally early activity in antioxidant enzymes such as superoxide dismutase (SOD). However, the mutant leaves showed lower catalase (CAT) activity. By contrast, guaiacol peroxidase (G-POD) activities were higher in the mutant than in the wild type roots. Together with the Cd toxicity-induced decline of early responsive enzymatic activities in vivo, especially CAT, the inability of mutants to scavenge accumulated H2O2 resulted in higher lipid peroxide levels. H2O2 might also strengthen the expression of G-POD as a signaling molecule. Results suggest that G-POD activity can be a potential biomarker reflecting Cd sensitivity in rice seedlings. Key words: Antioxidant enzyme, Cd toxicity, Cd-sensitive mutant, lipid peroxidation, rice (Oryza sativa L.)

scholarly journals Low pH-Induced Changes of Antioxidant Enzyme and ATPase Activities in the Roots of Rice (Oryza sativa L.) Seedlings

PLoS ONE ◽  
2015 ◽  
Vol 10 (2) ◽  
pp. e0116971 ◽  
Author(s):  
Yi-Kai Zhang ◽  
De-Feng Zhu ◽  
Yu-Ping Zhang ◽  
Hui-Zhe Chen ◽  
Jing Xiang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Antioxidant Enzyme ◽  
Oryza Sativa L ◽  
Low Ph ◽  
Induced Changes

Copper and Inhibitors Induced Changes on Subcellular Antioxidant Enzyme Activities in the Leaves of Elsholtzia haichowensis

2012 ◽  
Vol 518-523 ◽  
pp. 5539-5544
Author(s):  
Dong Yuan
Keyword(s):  
Antioxidant Enzymes ◽  
Nadph Oxidase ◽  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Defense Mechanism ◽  
Additional Treatment ◽  
Antioxidant Enzyme Activities ◽  
Cell Compartments ◽  
Elsholtzia Haichowensis ◽  
Induced Changes

Under hydroponics, shoots of the copper (Cu) accumulator Elsholtzia haichowensis after 100 μM CuSO4 treatment 6 day were additionally treated with different inhibitors, then the effects of Cu and inhibitors on antioxidant enzyme activities in leave cells of E. haichowensis were investigated. Our results showed that Cu treatment significantly increased the activities of plasma membrane–bound NADPH oxidase, apoplastic peroxidase, apoplastic superoxide dismutase (SOD), symplastic SOD and symplastic ascorbate peroxidase in leaves of E. haichowensis. When additional treatment with N-N-diethyldithiocarbamate as an inhibitor of SOD, NaN3 as an inhibitor of peroxidase, diphenyleneiodonium as an inhibitor of NADPH oxidase and1,2-dihydroxybenzene-3,5-disulphonic acid as an O2•– scavenger, the activities of Cu-induced antioxidant enzymes in leave cells of E. haichowensis were significantly inhibited. The results showed that Cu-induced increase in the activities of various antioxidant enzymes in different cell compartments may represent a defense mechanism against oxidative stress.

scholarly journals Wound-Induced Changes in Antioxidant Enzyme Activities in Skin Mucus and in Gene Expression in the Skin of Gilthead Seabream (Sparus aurata L.)

Fishes ◽  
2021 ◽  
Vol 6 (2) ◽  
pp. 15
Author(s):  
Cristóbal Espinosa-Ruíz ◽  
María Ángeles Esteban
Keyword(s):  
Antioxidant Enzyme ◽  
Enzyme Activities ◽  
Stress Proteins ◽  
Sparus Aurata ◽  
Gilthead Seabream ◽  
Antioxidant Enzyme Activities ◽  
Skin Mucus ◽  
Expression Levels ◽  
Induced Changes ◽  
Related Proteins

This study investigated the antioxidant enzyme activities in the skin mucus of gilthead seabream (Sparus aurata L.) at 3 and 7 days post-wounding (dpw). The expression levels of the genes that encode stress proteins (grp170, grp94, grp75, sod and hsp70) and skin regeneration-related proteins (tf, igf1, tgfb1, der1, apo1 and erdj3) in the skin also were determined. Mucus and skin samples were obtained from the left and right flanks of non-wounded and wounded fish. In both flanks of the wounded fish, catalase and glutathione reductase activities in the skin mucus increased (p < 0.05) at 3 and 7 dpw (100 ± 31% and 111 ± 25%, respectively), whereas superoxide dismutase activity increased (p < 0.05) only at 7 dpw (135 ± 15%). The expression levels of stress proteins in the skin of the wounded flank of the wounded fish mainly increased at 7 dpw (grp170 increased to 288 ± 85%, grp94 to 502 ± 143%, grp75 to 274 ± 69%, sod to 569 ± 99%, and hsp70 increased to 537 ± 14%) (p < 0.05). However, the expression levels of the tissue regeneration-related genes varied depending on the flank investigated, on the experimental time, and on the gene studied. To the best of our knowledge, this is the first work to determine the effect of a wound in different skin parts of the same fish.

Sign in / Sign up

Export Citation Format

Share Document