Regulation of cell-wall polysaccharide components by CaCl2 in suspension cultures of kidney bean (Phaseolus vulgaris)

2002 ◽  
Vol 45 (2) ◽  
pp. 90-95 ◽  
Author(s):  
Up-Dong Yeo ◽  
Kyong-Ho Kim
Keyword(s):  
Cell Wall ◽  
Phaseolus Vulgaris ◽  
Suspension Cultures ◽  
Kidney Bean ◽  
Cell Wall Polysaccharide

scholarly journals Cell wall polysaccharide biosynthesis and related metabolism in elicitor-stressed cells of French bean (Phaseolus vulgaris L.)

1995 ◽  
Vol 306 (3) ◽  
pp. 745-750 ◽  
Author(s):  
D Robertson ◽  
B A McCormack ◽  
G P Bolwell
Keyword(s):  
Cell Wall ◽  
Phaseolus Vulgaris ◽  
Time Course ◽  
Glucose Dehydrogenase ◽  
French Bean ◽  
Primary Cell Wall ◽  
Cell Wall Polysaccharide ◽  
Phaseolus Vulgaris L ◽  
Glucan Synthase ◽  
Elicitor Treatment

Enzyme activities involved in quantitative and qualitative flux of sugars into cell wall polysaccharides were determined following elicitor treatment of suspension cultured cells of French bean (Phaseolus vulgaris L.). Two subsets of activities were examined: the first were involved in synthesis and metabolism of UDP-glucose and the provision of the pool of UDP-sugars, and the second a selection of membrane-bound glycosyltransferases involved in the synthesis of pectins, hemicelluloses and glucans of the primary cell wall. Of the first group, only UDP-glucose dehydrogenase (EC 1.1.1.22) showed any significant induction in response to elicitor treatment, sucrose synthase (EC 2.4.1.13), UDP-glucuronate decarboxylase (EC 4.1.1.35), UDP-glucose and UDP-xylose 4-epimerases (EC 5.1.3.2 and EC 5.1.3.5 respectively) did not change in activity significantly over the time course. In contrast, enzymes of the second group showed a more complex response. Callose synthase (glucan synthase II, EC 2.4.1.12) increased in activity, as has been shown in other systems, while arabinan synthase (EC 2.4.1.-), xylan synthase (EC 2.4.1.72), xyloglucan synthase (EC 2.4.1.72) and glucan synthase I (EC 2.4.1.12) activities were rapidly depleted from membranes within 3 h following elicitor action. This rapid turnover of activity was striking, indicating that the half-life of such enzymes can be short and that elicitor action causes substantial perturbation of some membrane activities. Glucan synthase I activity appears to increase in the later stages over the time period measured, indicating some recovery of this metabolism.

Two peroxidases isolated from kidney bean cell suspension cultures

1972 ◽  
Vol 50 (6) ◽  
pp. 1245-1252 ◽  
Author(s):  
M. Misawa ◽  
S. M. Martin
Keyword(s):  
Molecular Weight ◽  
Phaseolus Vulgaris ◽  
Gel Filtration ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Kidney Bean ◽  
Molecular Weights ◽  
A Cell ◽  
Plant Peroxidases ◽  
Red Kidney Bean

Two peroxidases, isolated from filtrates of suspension cultures of a cell line derived from red kidney bean (Phaseolus vulgaris) were purified 145- and 72-fold respectively. The two enzymes were quite similar in many of their properties and both were typical plant peroxidases. They differed markedly, however, in their molecular weights (estimated by gel filtration). The molecular weight of peroxidase I was 30 000 whereas that of peroxidase II was only 6000.

scholarly journals Crystal structure to 2.45 Å resolution of a monoclonal Fab specific for theBrucellaA cell wall polysaccharide antigen

1993 ◽  
Vol 2 (7) ◽  
pp. 1106-1113 ◽  
Author(s):  
D. R. Rose ◽  
M. Przybylska ◽  
R. J. To ◽  
C. S. Kayden ◽  
E. Vorberg ◽  
...  
Keyword(s):  
Crystal Structure ◽  
Cell Wall ◽  
Cell Wall Polysaccharide ◽  
Polysaccharide Antigen

scholarly journals PUL-Mediated Plant Cell Wall Polysaccharide Utilization in the Gut Bacteroidetes

2021 ◽  
Vol 22 (6) ◽  
pp. 3077
Author(s):  
Zhenzhen Hao ◽  
Xiaolu Wang ◽  
Haomeng Yang ◽  
Tao Tu ◽  
Jie Zhang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Microbial Community ◽  
Gut Microbiota ◽  
Plant Cell ◽  
Plant Cell Wall ◽  
Cell Wall Polysaccharide ◽  
Prominent Feature ◽  
Cell Wall Polysaccharides ◽  
Gut Microbial Community

Plant cell wall polysaccharides (PCWP) are abundantly present in the food of humans and feed of livestock. Mammalians by themselves cannot degrade PCWP but rather depend on microbes resident in the gut intestine for deconstruction. The dominant Bacteroidetes in the gut microbial community are such bacteria with PCWP-degrading ability. The polysaccharide utilization systems (PUL) responsible for PCWP degradation and utilization are a prominent feature of Bacteroidetes. In recent years, there have been tremendous efforts in elucidating how PULs assist Bacteroidetes to assimilate carbon and acquire energy from PCWP. Here, we will review the PUL-mediated plant cell wall polysaccharides utilization in the gut Bacteroidetes focusing on cellulose, xylan, mannan, and pectin utilization and discuss how the mechanisms can be exploited to modulate the gut microbiota.

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