Effect of humidity on somatic embryogenesis in cotyledon explant culture of carrot

1997 ◽  
Vol 40 (2) ◽  
pp. 89-94 ◽  
Author(s):  
Eun Kyong Lee ◽  
Duck Yee Cho ◽  
Woong Young Soh
Keyword(s):  
Somatic Embryogenesis ◽  
Explant Culture ◽  
Cotyledon Explant

scholarly journals EFFECT OF EXPLANTS AND GROWTH REGULATORS ON SOMATIC EMBRYO-GENESIS AND ADVENTITIOUS ORGANOGENESIS IN CAPSICUM ANNUUM

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 618c-618 ◽  
Author(s):  
Hyo-Geur. Park ◽  
Kyung-Young Choi ◽  
Do-Hyun Lee
Keyword(s):  
Tissue Culture ◽  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Growth Regulators ◽  
Hot Pepper ◽  
Ms Medium ◽  
Free Medium ◽  
Explant Source ◽  
Morphogenic Response ◽  
Cotyledon Explant

Hot pepper is the most important vegetable in Korea in terms of both acreage and product value. Morphogenic response in tissue culture of hot pepper varied greatly with explant source and growth regulators. Somatic enbryogenesis, which we believe had not been reported yet, was only induced from 5-month-old callus derived from anther culture. Embryogenic callus was solely observed in the MS medium supplemented with 2 mg/l of NAA and 2 mg/l of BA. Better somatic embryogenesis and plant regeneration was observed on hormone free medium. Adventitious organogenesis was well induced from both hypocotyl and cotyledon. Polarity of explant for shoot regeneration was observed. Success rate of regeneration has been continuously improved mainly due to combinations of growth regulators. The best combination so far was 2 mg/l of zeatin and 1 mg/l of IAA, resulting in 92% of regeneration from cotyledon explant.

Somatic embryogenesis of Cyclamen persicum in liquid medium

1995 ◽  
Vol 94 (4) ◽  
pp. 605-612 ◽  
Author(s):  
Marc Kreuger ◽  
Erik Postma ◽  
Yvon Brouwer ◽  
Gerrit-Jan van Holst
Keyword(s):  
Somatic Embryogenesis ◽  
Liquid Medium ◽  
Cyclamen Persicum

Ovarian carcinoma explant culture: model development and application in drug testing

2016 ◽  
Vol 76 (10) ◽  
Author(s):  
M Suarez-Carmona ◽  
A Heinzelmann ◽  
M Hampel ◽  
S Schott ◽  
I Zörnig ◽  
...  
Keyword(s):  
Ovarian Carcinoma ◽  
Drug Testing ◽  
Model Development ◽  
Explant Culture ◽  
Culture Model

Somatic Embryogenesis and In vitro Plant Regeneration in Vigna trilobata (L.) Verd. - A Potential Pasture Legume

1970 ◽  
Vol 19 (1) ◽  
pp. 89-99
Author(s):  
K. Choudhary ◽  
M. Singh ◽  
M. S. Rathore ◽  
N. S. Shekhawat
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Long Term Study ◽  
Continuous Application ◽  
First Time ◽  
Pasture Legume

This long term study demonstrates for the first time that it is possible to propagate embryogenic Vigna trilobata and to subsequently initiate the differentiation of embryos into complete plantlets. Initiation of callus was possible on 2,4-D. Somatic embryos differentiated on modified MS basal nutrient medium with 1.0 mg/l  of 2,4-D and 0.5 mg/l  of Kn. Sustained cell division resulted in globular and heart shape stages of somatic embryos. Transfer of embryos on to a fresh modified MS basal medium with 0.5 mg/l of Kn and 0.5 mg/l of GA3 helped them to attain maturation and germination. However, the propagation of cells, as well as the differentiation of embryos, were inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking these growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation of embryogenic cultures in Vigna species are discussed. Key words: Pasture  legume, Vigna trilobata, Globular, Heart shape, somatic embryogenesis D.O.I. 10.3329/ptcb.v19i1.4990 Plant Tissue Cult. & Biotech. 19(1): 89-99, 2009 (June)

Somatic Embryogenesis and Plant Development in Eight Cultivars of Pecan

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 573-576 ◽  
Author(s):  
I.E. Yates ◽  
C.C. Reilly
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Development ◽  
Fruit Development ◽  
Somatic Embryos ◽  
Carya Illinoensis ◽  
Appreciable Variation

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.

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