Change of insulin-like growth factor gene expression in Chinese hamster ovary cells cultured in serum-free media

2006 ◽  
Vol 11 (4) ◽  
pp. 319-324 ◽  
Author(s):  
Hongwoo Park ◽  
Sungkwan An ◽  
Taeboo Choe
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Factor Gene ◽  
Growth Factor Gene ◽  
Free Media ◽  
Cells Cultured

Effects of phospholipids on growth of Chinese hamster ovary cells in serum-free media

1999 ◽  
Vol 88 (3) ◽  
pp. 306-309 ◽  
Author(s):  
Kentaro Sakai ◽  
Toshio Matsunaga ◽  
Hideki Yamaji ◽  
Hideki Fukuda
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Ovary Cells ◽  
Serum Free ◽  
Free Media

scholarly journals Recombinant type 1 transforming growth factor beta precursor produced in Chinese hamster ovary cells is glycosylated and phosphorylated.

1988 ◽  
Vol 8 (5) ◽  
pp. 2229-2232 ◽  
Author(s):  
A M Brunner ◽  
L E Gentry ◽  
J A Cooper ◽  
A F Purchio
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Chinese Hamster Ovary ◽  
Transforming Growth Factor ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Tgf Beta ◽  
Ovary Cells ◽  
Growth Factor Beta

Analyses of cDNA clones coding for simian type 1 transforming growth factor beta (TGF-beta 1) suggest that there are three potential sites for N-linked glycosylation located in the amino terminus of the precursor region. Analysis of [3H]glucosamine-labeled serum-free supernatants from a line of Chinese hamster ovary cells which secrete high levels of recombinant TGF-beta 1 indicate that the TGF-beta 1 precursor, but not the mature form, is glycosylated. Digestion with neuraminidase resulted in a shift in migration of the two TGF-beta 1 precursor bands, which suggests that they contain sialic acid residues. Endoglycosidase H had no noticeable effect. Treatment with N-glycanase produced two faster-migrating sharp bands, the largest of which had a molecular weight of 39 kilodaltons. TGF-beta 1-specific transcripts produced by SP6 polymerase programmed the synthesis of a 42-kilodalton polypeptide which, we suggest, is the unmodified protein backbone of the precursor. Labeling with 32Pi showed that the TGF-beta 1 precursor was phosphorylated in the amino portion of the molecule.

scholarly journals Mild Hypothermia Improves Transient Gene Expression Yields Several Fold in Chinese Hamster Ovary Cells

2008 ◽  
Vol 24 (2) ◽  
pp. 458-465 ◽  
Author(s):  
S. Wulhfard ◽  
S. Tissot ◽  
S. Bouchet ◽  
J. Cevey ◽  
M. DeJesus ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chinese Hamster Ovary ◽  
Mild Hypothermia ◽  
Chinese Hamster Ovary Cells ◽  
Transient Gene Expression ◽  
Chinese Hamster ◽  
Ovary Cells
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