Hormonal and extracellular matrix regulation of plasminogen activator in a bovine mammary epithelial cell line

Endocrine ◽  
1995 ◽  
Vol 3 (5) ◽  
pp. 345-350 ◽  
Author(s):  
Ioannis Politis ◽  
Boris Zavizion ◽  
Jeffrey H. White ◽  
John J. Goldberg ◽  
Antonella Baldi ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Cell Line ◽  
Plasminogen Activator ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Bovine Mammary Epithelial Cell

Effects of retinoids on proliferation and plasminogen activator expression in a bovine mammary epithelial cell line

2003 ◽  
Vol 70 (4) ◽  
pp. 367-372 ◽  
Author(s):  
Federica Cheli ◽  
Ioannis Politis ◽  
Luciana Rossi ◽  
Eleonora Fusi ◽  
Antonella Baldi
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Cell Line ◽  
Plasminogen Activator ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mrna Levels ◽  
Mammary Epithelial Cell Line ◽  
Bovine Mammary Epithelial Cell

Effects of two natural (retinol and retinoic acid, RA) and one synthetic N-(4-hydroxyphenyl) retinamide (4-HPR) retinoids on proliferation and expression of urokinase-plasminogen activator (u-PA) by bovine mammary epithelial cells were examined. The BME-UV1 established bovine mammary epithelial cell line was used as a model system. All retinoids tested (retinol, RA and 4-HPR) were effective inhibitors of cell proliferation. When cells were cultured in the absence of fetal bovine calf serum (FBCS), inhibition occurred at concentrations as low as 1 nM for all retinoids tested. The effect of retinoids on cell proliferation was not dose-related when cells were cultured in the absence of FBCS. All retinoids (retinol, RA, 4-HPR), when used in the range 1 nM–10 μM (noncytotoxic concentrations), were equally effective and had identical inhibition patterns. Inhibition of cell proliferation by RA was apparent by 6 h and was higher after 24 h in culture. In contrast, when cells were cultured in the presence of FBCS, the effect of RA and retinol on cell proliferation was dose-related. RA and retinol inhibited cell proliferation (P<0·01) when added to the culture medium in concentrations as low as 10 nM and 100 nM, respectively. 4-HPR was inhibitory (P<0·01) in concentrations as low as 1 nM. Higher concentrations of 4-HPR in the range 1 nM–1 μM had no further effect on cell proliferation. None of the retinoids tested, when added to cultures in the presence or absence of FBCS, could completely arrest cell proliferation at noncytotoxic concentrations. RA at 1 μM inhibited (P<0·05) insulin or IGF-I-induced cell proliferation but had no effect (P>0·05) on u-PA mRNA levels or u-PA activity. Furthermore, RA inhibited cell proliferation in the presence of FBCS but had no effect (P>0·05) on u-PA mRNA levels. Thus, retinoids are effective inhibitors of bovine mammary epithelial cell proliferation and this growth inhibition does not seem to correlate with any changes in u-PA mRNA or u-PA activity.

scholarly journals Stable infection of a bovine mammary epithelial cell line (MAC-T) with bovine leukemia virus (BLV)

2018 ◽  
Vol 256 ◽  
pp. 11-16 ◽  
Author(s):  
Lucia Martinez Cuesta ◽  
Maria Victoria Nieto Farias ◽  
Pamela Anahi Lendez ◽  
Lucas Barone ◽  
Sandra Elizabeth Pérez ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Bovine Leukemia Virus ◽  
Mammary Epithelial Cell ◽  
Leukemia Virus ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Bovine Mammary Epithelial Cell

Receptor binding and growth-promoting activity of insulin-like growth factor-I in a bovine mammary epithelial cell line (MAC-T3)

1992 ◽  
Vol 134 (2) ◽  
pp. 307-312 ◽  
Author(s):  
X. Zhao ◽  
B. W. McBride ◽  
I. Politis ◽  
H. T. Huynh ◽  
R. M. Akers ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Mammary Epithelial Cell ◽  
Mammary Epithelial Cells ◽  
Mammary Epithelial ◽  
Epithelial Cell Line ◽  
Mammary Epithelial Cell Line ◽  
Bovine Mammary Epithelial Cells ◽  
Bovine Mammary Epithelial Cell ◽  
Igf I

ABSTRACT Insulin-like growth factor-I (IGF-I) has been known to be mitogenic to a variety of cell types, although a growth-regulatory role for IGF-I on bovine mammary epithelial cells has not been fully investigated. In the present study, we examined the receptor binding of IGF-I and its effect on growth in a bovine mammary epithelial cell line (MAC-T3). Specific receptors for IGF-I were detected on cultured bovine mammary epithelial cells. Competitive binding revealed that half-maximal inhibition of 125I-labelled IGF-I binding by IGF-I was approximately 3 μg/l. Dissociation rate constant of the IGF-I receptor was 3·10±0·06 nmol/l (s.e.m.) with a receptor site concentration of 366 ± 8 fmol/mg protein for the average of three experiments. IGF-I exerted a positive mitogenic effect on MAC-T3 cells according to both direct DNA assay and thymidine incorporation assay. Moreover, the mitogenic effect of IGF-I on MAC-T3 cells was enhanced by the addition of fetal calf serum in the culture media. The present results suggest that the bovine mammary epithelial cell line (MAC-T3) provides a useful model system with which to study the biological actions of insulin-like growth factors on the bovine mammary secretory tissue in vitro. Journal of Endocrinology (1992) 134, 307–312

Sign in / Sign up

Export Citation Format

Share Document