Chromosome banding by restriction enzyme digestion distinguishes between Mus domesticusand Mus musculuskaryotypes

1992 ◽  
Vol 3 (3) ◽  
pp. 247-255 ◽  
Author(s):  
Silvia Garagna ◽  
Carlo Alberto Redi ◽  
Paola Veneroni ◽  
Ernesto Capanna ◽  
E. Capanna
Keyword(s):  
Restriction Enzyme ◽  
Chromosome Banding ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion

scholarly journals Endonuclease banding reveals that atrazine-induced aneuploidy resembles spontaneous chromosome loss in Crassostrea gigas

Genome ◽  
2005 ◽  
Vol 48 (1) ◽  
pp. 177-180 ◽  
Author(s):  
Karine Bouilly ◽  
Alexandra Leitão ◽  
Raquel Chaves ◽  
Henrique Guedes-Pinto ◽  
Pierre Boudry ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Crassostrea Gigas ◽  
Chromosome Banding ◽  
Pacific Oyster ◽  
Enzyme Digestion ◽  
Banding Technique ◽  
Chromosome Loss ◽  
Restriction Enzyme Digestion ◽  
The Pacific ◽  
Spontaneous Chromosome

Aneuploidy has previously been observed in the Pacific oyster, Crassostrea gigas, and shown to be negatively correlated with growth. Moreover, a significant impact of atrazine exposure has been described in C. gigas, and persistence of that effect has been observed between generations. Evidence of differential chromosome loss has been demonstrated in aneuploid karyotypes of C. gigas using the G-banding technique. Pairs 1, 5, 9, and 10 are characterized by the loss of 1 chromosome. As restriction enzyme (RE) digestion chromosome banding allows a better identification of chromosome pairs, we used this technique to identify which chromosomes are affected when aneuploidy is increased by exposure to atrazine. The progeny of oysters contaminated by atrazine were analysed using the restriction enzyme HaeIII. The study of 26 RE-banded aneuploid karyotypes showed that the same chromosome pairs (1, 5, 9, and 10) were affected by the loss of 1 chromosome (61%, 15%, 42%, and 42%, respectively). Further investigation is required to enable a better understanding of aneuploidy in oysters, especially with respect to why some chromosomes are more easily lost than others, and why cells tolerate the loss of these chromosomes.Key words: aneuploidy, atrazine, restriction enzyme digestion chromosome banding, Pacific oyster, Crassostrea gigas.

The stability of maize nuclei to restriction enzyme digestion differs with transcriptional activity

Caryologia ◽  
1993 ◽  
Vol 46 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Valeria Mirkova ◽  
Maria Ivanchenko ◽  
Lubomir Stoilov ◽  
Jordanka Zlatanova
Keyword(s):  
Restriction Enzyme ◽  
Transcriptional Activity ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
The Stability

Identification of the CD32/FcγRIIc-Q13/STP13 polymorphism using an allele-specific restriction enzyme digestion assay

2001 ◽  
Vol 258 (1-2) ◽  
pp. 85-95 ◽  
Author(s):  
D Metes ◽  
A.A Gambotto ◽  
J Nellis ◽  
A Ruscin ◽  
A.M Stewart-Akers ◽  
...  
Keyword(s):  
Restriction Enzyme ◽  
Enzyme Digestion ◽  
Restriction Enzyme Digestion ◽  
Allele Specific ◽  
Specific Restriction
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