scholarly journals Application of electron energy loss spectroscopy and electron spectroscopic imaging to aluminum development in biological tissue

1994 ◽  
Vol 42 (1) ◽  
pp. 80-80
Author(s):  
Xuezheng Xie ◽  
Robert A. Yokel ◽  
William R. Markesbery
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Biological Tissue ◽  
Spectroscopic Imaging ◽  
Electron Spectroscopic Imaging ◽  
Electron Energy Loss

scholarly journals Ultrastructural distribution of terbium across capillary endothelium: detection by electron spectroscopic imaging and electron energy loss spectroscopy.

1990 ◽  
Vol 38 (2) ◽  
pp. 275-282 ◽  
Author(s):  
R C Wagner ◽  
S C Chen
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Electron Energy Loss Spectroscopy ◽  
Spectroscopic Imaging ◽  
Capillary Endothelium ◽  
Electron Spectroscopic Imaging ◽  
Thin Sections ◽  
Rete Mirabile ◽  
Heavy Electron ◽  
Electron Energy Loss

We used terbium as an intravital tracer of permeability pathways across the walls of capillaries in the rete mirabile of the eel swimbladder and in frog mesentery. Terbium was detected in unstained ultra-thin sections by electron density using electron spectroscopic imaging (ESI) and by electron energy loss spectroscopy (EELS). Enhancement of intrinsic contrast in zero loss images (elastically scattered electrons) permitted imaging of membrane-bound compartments and terbium within them which might otherwise have been undetected in counterstained sections. Element-selective imaging with EELS indicated that terbium was associated with heavy electron-dense deposits, but the terbium mass:volume of sections in areas of lighter deposition was insufficient to obtain a terbium signal. In the rete capillaries, terbium was deposited on the luminal surface, throughout vesicular profiles, and in the interstitium, but could not be traced through interendothelial junctions. Fine terbium deposits were detectable throughout apparent vesicular connections across the endothelium. In the frog mesentery, terbium penetrated some but not all interendothelial clefts, and was detectable in small quantities within luminal and abluminal vesicular profiles and in the interstitium. The results indicate that in the rete capillaries, terbium permeates the capillary via a transcellular route. This route may be provided by transient fusions of luminal and abluminal vesicular compartments.

scholarly journals Electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) of multilamellar bodies and multilamellar body-like structures in tannic acid-treated alveolar septal cells.

1994 ◽  
Vol 42 (6) ◽  
pp. 805-809 ◽  
Author(s):  
M Ochs ◽  
H Fehrenbach ◽  
J Richter
Keyword(s):  
Energy Loss ◽  
Electron Energy ◽  
Tannic Acid ◽  
Electron Energy Loss Spectroscopy ◽  
Spectroscopic Imaging ◽  
Structural Similarity ◽  
Alveolar Epithelial Cells ◽  
Electron Spectroscopic Imaging ◽  
Multilamellar Bodies ◽  
Electron Energy Loss

We used electron spectroscopic imaging (ESI) and electron energy loss spectroscopy (EELS) to compare multilamellar bodies (MLB) of Type II alveolar epithelial cells with MLB-like structures that are present in various alveolar septal cells after fixation with tannic acid. Despite their structural similarity in conventional transmission electron microscopy, the phosphorus signal recorded by both ESI and EELS was considerably higher in multilamellar bodies than in MLB-like structures. This indicates that they are different in chemical composition.

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