New semiquantitative assessment of123I-FP-CIT by an anatomical standardization method

2006 ◽  
Vol 20 (7) ◽  
pp. 477-484 ◽  
Author(s):  
Seiko Takada ◽  
Mana Yoshimura ◽  
Hiroaki Shindo ◽  
Kazuhiro Saito ◽  
Kiyoshi Koizumi ◽  
...  
Author(s):  
Matthew J. Bicocca ◽  
Emma J. Qureshey ◽  
Suneet P. Chauhan ◽  
Edgar Hernandez‐Andrade ◽  
Baha M. Sibai ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (5) ◽  
pp. e0125713 ◽  
Author(s):  
Takeshi Hara ◽  
Tatsunori Kobayashi ◽  
Satoshi Ito ◽  
Xiangrong Zhou ◽  
Tetsuro Katafuchi ◽  
...  

2018 ◽  
Vol 2018 ◽  
pp. 1-3
Author(s):  
Carol E. Muenks ◽  
Patrick G. Hogan ◽  
Carey-Ann D. Burnham ◽  
Stephanie A. Fritz

Given the lack of standardization of methodologies for microbial recovery from built environments, we sought to compare the yield of Staphylococcus aureus with a broth enrichment method when incubated in agitated versus static conditions. Five unique strains of S. aureus at five different concentrations were cultured to compare direct plating, agitated broth enrichment, and static broth enrichment culture methods. All samples were incubated at 35° in ambient air. The lowest concentration recovered across three replicates and five strains did not differ between culture methods (Fisher’s exact test, p=0.50); notably, recovery of S. aureus was equivalent between static and agitated broth incubation. When broth enrichment was used (both static and agitated), the burden of S. aureus growth was higher (by semiquantitative assessment of 4-quadrant streaking) compared to the direct plating culture method. Optimizing strategies for microbial recovery is essential, particularly in areas of lower biomass, given the paucity of research concerning microbial communities of built environments. The results of this study, in conjunction with other experiments investigating microbiomes of built environments, can help inform protocols for standardizing culturing methods within built environments.


2005 ◽  
Vol 12 (1) ◽  
pp. 218-223 ◽  
Author(s):  
Daniel J. Sikkema ◽  
Nancy A. Ziembiec ◽  
Thomas R. Jones ◽  
Stephen W. Hildreth ◽  
Dace V. Madore ◽  
...  

ABSTRACT Weight-based assignments for immunoglobulin G1 (IgG1) and IgG2 subclass antibodies to Streptococcus pneumoniae capsular polysaccharides (PnPs) in antipneumococcal standard reference serum lot 89-S (lot 89-S), also known as lot 89-SF, have been determined for serotypes 1, 4, 5, 7F, 9V, and 18C. This extends the usefulness of lot 89-S beyond the IgG1 and IgG2 subclass assignments for serotypes 3, 6B, 14, 19F, and 23F made previously (A. Soininen, H. Kayhty, I. Seppala, and T. Wuorimaa, Clin. Diagn. Lab. Immunol. 5:561-566, 1998) to cover 11 major serotypes associated with the highest percentage of pneumococcal disease worldwide. A method of equivalence of absorbances in enzyme immunosorbent assays was used to determine the IgG1 and IgG2 antibody concentrations for the additional serotypes in lot 89-S, based on the subclass values previously assigned for PnPs serotypes 6B, 14, and 23F. This cross-standardization method assures consistency with previous antibody assignments in that reference serum. The newly assigned subclass values for serotype 9V, and previously assigned values for serotype 14, were used to quantitate PnPs antibodies in sera from adult and pediatric subjects immunized with a pneumococcal conjugate vaccine. There was a predominance of IgG1 anti-PnPs antibodies in pediatric sera and IgG2 anti-PnPs antibodies in the adult sera. The IgG1 and IgG2 subclass assignments for the 11 PnPs serotypes in antipneumococcal standard reference serum lot 89-S are useful for quantitating and characterizing immune responses to pneumococcal infection and vaccination regimens.


2010 ◽  
Vol 287 (2) ◽  
pp. 629-634 ◽  
Author(s):  
Mohamed Soliman ◽  
Nader M. A. Mohamed ◽  
M. A. Gaheen ◽  
E. A. Saad ◽  
S. K. Yousef ◽  
...  

2020 ◽  
Vol 134 (4) ◽  
pp. 469-471
Author(s):  
Xin Gao ◽  
Qing-Tao Hui ◽  
Yu-Dan Li ◽  
Miao Peng ◽  
Dan Xu ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document