Long-Term Engraftment Stability of Peripheral Blood Stem Cells Cryopreserved Using the Dump-Freezing Method in a −80°C Mechanical Freezer with 10% Dimethyl Sulfoxide

2001 ◽  
Vol 73 (2) ◽  
pp. 245-250 ◽  
Author(s):  
Chul Won Choi ◽  
Byung Soo Kim ◽  
Jae Hong Seo ◽  
Sang Won Shin ◽  
Yeul Hong Kim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dimethyl Sulfoxide ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Freezing Method ◽  
Blood Stem Cells

Automated washing of long-term cryopreserved peripheral blood stem cells promotes cell viability and preserves CD34+ cell numbers

2018 ◽  
Vol 53 (9) ◽  
pp. 1225-1227
Author(s):  
Fatima Aerts-Kaya ◽  
Gül Koca ◽  
Parisa Sharafi ◽  
Funda Çakmak Sayla ◽  
Duygu Uçkan-Çetinkaya ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cell Viability ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Cell Numbers ◽  
Blood Stem Cells

The Disease Status at Transplant Is the Major Factor Association with the Long Term Survival of HLA Haploidentical Peripheral Blood Stem Cells Transplantation.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 5107-5107
Author(s):  
Binyi Wu ◽  
Lanxiao Wu ◽  
Kunyuan Guo ◽  
Chaoyang Song ◽  
Dingan Yan ◽  
...  
Keyword(s):  
Stem Cells ◽  
Graft Versus Host Disease ◽  
Peripheral Blood ◽  
Peripheral Blood Stem Cells ◽  
Term Survival ◽  
Long Term Survival ◽  
Graft Versus Host ◽  
Blood Stem Cells ◽  
Stem Cells Transplantation

Abstract Objective: To evaluate the factors effecting the long term survival of refractory leukemia patients who received the therapy of HLA haploidentical peripheral blood stem cells transplantation. Methods: To analysis the factors effecting long term survival of refractory leukemia patients who underwent HLA haploidentical peripheral blood stem cells transplantation. The HLA mismatched locus between the donors and patients, the disease status of patients at transplant, the grafted mononucleaer cells number, and the occurrence of GVHD, the age of patients and other factors were considered and analyzed by data reduction SSP11. From July, 1998 to May, 2004, 30 Patients with refractory leukemia patients including 13 cases of acute non-lymphocytes leukemia, 10 cases of acute lymphocytes leukemia, 6 cases chronic myeloid leukemia and 1 cases of non-Hodgkin lymphoma underwent HLA haploidentical peripheral blood stem cells transplantations. The median age was 25 years old (3– 52 years old). Twelve patients received stem cells from parents, four from daughter and son, and the other from sibling donors. Twelve patients received three HLA locus mismatched stem cells, thirteen patients received two HLA mismatched donors stem cells, and five patients received one HLA mismatched donors stem cells. The conditioning regime consisted of fludara (25mg/m2 X5d), busufan (4mg/kg X4d) and cyclophosphamide ( 60mg/kg X2d). Median dose of rabbit anti-human lymphocyte globulin (5mg/kg X5d) was added in the in some patients. A mean of 6.0 x 108 /kg (3–9 x108 /kg) mono-nucleated cells was grafted. The mean CD34+ cells number was 5.5 x106 /Kg (3–6.5 x106/kg). Results: Twenty-nine patients were successfully grafted and one failed to graft. The mean time of white cell count more than 1x109/L was 13 days (10–18 days) and 12 days (9–16) respectively. Severe acute graft versus host disease occurred in six patients, and four died. Seven patients suffered from intensive chronic graft versus host disease. Nine patients relapsed and died. The mean relapse time was 10 months (3 months to 24 months). Four patients died from intensive chronic graft versus host disease. Fourteen patients are still disease free survival with high karnofsky performs. The relapse of leukemia was the main cause of death. Five the patients less than 20 year’s old age are still disease free survival with high karnofsky performs scores. Conclusion: HLA haploidentical peripheral blood stem cells transplantation may be an effect therapy for refractory leukemia. Although graft versus leukemia effect may be strong in HLA haploidentical blood stem cells transplantation, leukemia relapse is still the main cause to death. We suggest that for these patients with leukemia who can not find full matched donor perform related HLA mismatched peripheral blood stem cells transplantation as earlier as to get the better long term outcome.

Dimethyl Sulfoxide-Induced Tonic-Clonic Seizure and Cardiac Arrest During Infusion of Autologous Peripheral Blood Stem Cells

2018 ◽  
Vol 19 (4) ◽  
pp. 831-832 ◽  
Author(s):  
Senem Maral ◽  
Murat Albayrak ◽  
Cigdem Pala ◽  
Abdulkerim Yildiz ◽  
Osman Sahin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cardiac Arrest ◽  
Dimethyl Sulfoxide ◽  
Peripheral Blood ◽  
Clonic Seizure ◽  
Tonic Clonic Seizure ◽  
Peripheral Blood Stem Cells ◽  
Blood Stem Cells

The Long Term Survival of the Patients with Refractory Leukemia Treated by Related HLA Haploidentical Stem Cells Transplantation.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 5388-5388
Author(s):  
Bingyi Wu ◽  
Guo Kunyuan ◽  
Haiyan Hu ◽  
Yuliang Yang ◽  
Lan Deng
Keyword(s):  
Stem Cells ◽  
Peripheral Blood ◽  
Disease Free Survival ◽  
Peripheral Blood Stem Cells ◽  
Term Survival ◽  
Free Survival ◽  
Graft Versus Host ◽  
Blood Stem Cells ◽  
Stem Cells Transplantation

Abstract To assess the Long Term Survival of patients with refractory leukemia treated by HLA haploidentical stem cells transplantation. We analysed the outcomes of 35 patients with refractory leukemia who underwent HLA haploidentical peripheral blood stem cells transplantation from August, 1998 to December, 2005. Thirty five patients with refractory leukemia including 16 cases of acute non-lymphocytes leukemia, 11 cases of acute lymphocytes leukemia, 7 cases of chronic myeloid leukemia and 1 case of IV phase of non-Hodgkin lymphoma underwent HLA haploidentical peripheral blood stem cells transplantation. The median age was 25 years old (3– 52 years old). Fourteen patients received stem cells from parent donors, four from daughter and one from son donor, the others from sibling donors. Three HLA locus mismatched in thirteen cases, Two HLA locus mismatched donors in fifteen cases, One HLA locus mismatched in six cases. The conditioning regime consisted of fludara (25mg/m2 ×5d), busufan (4mg/kg ×4d) and cyclophsphamide(60mg/kg ×2d). Rabbit anti-human lymphocyte globulin (5mg/kg ×5d) was added in some patients in conditioning regime. A mean of 5.0 × 108/kg (2.97×108/kg-8.0×108/kg) mono-nucleated cells was grafted. The mean CD34+ cells number was 5.5 ×106/Kg (3–6.5 ×106/kg). Ten patients received 400ml donor derived bone marrow beside donor peripheral stem cells. Thirty-two patients were successfully grafted and one failed to graft and one died with severe fung infection at day 2, and one died from severe veno-occlusive disease at day 28. The mean time of white cell count more than 1×109/L was 14(11–18) days and platelet count more than 20×109/L was 15 (11–18) days. All patients got complete remission in 32 successful grafted patients. Severe acute graft versus host disease occurred in seven patients, and four died. Eight patients suffered from chronic graft versus host disease. Eight patients relapsed and died. The median relapse time was 10 (3–24) months. Fourteen patients are still disease free survival. The three years disease free survival rate was 40%. We concluded from our observation that HLA haploidentical peripheral blood stem cells transplantation may be an effective therapy for refractory leukemia. Some patients with refractory leukemia treated by HLA haploidentical stem cells transplantation could survive with free diseases; Though graft versus leukemia effect may be strong in HLA haploidentical blood stem cells transplantation, leukemia will probably be relapse when the patient without complete remission at transplant.

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