Purification and characterization of Cop, a protein involved in the copy number control of plasmid pE194

1998 ◽  
Vol 21 (3) ◽  
pp. 291-297 ◽  
Author(s):  
Jin-Hwan Kwak ◽  
Jungho Kim ◽  
Mu-Yong Kim ◽  
Eung-Chil Choi
Keyword(s):  
Copy Number ◽  
Purification And Characterization ◽  
Copy Number Control

scholarly journals Purification and characterization of RepA, a protein involved in the copy number control of plasmid pLS1

1989 ◽  
Vol 17 (7) ◽  
pp. 2405-2420 ◽  
Author(s):  
Gloria H. del Solar ◽  
Adela G. de la Campa ◽  
José Pérez-Martin ◽  
Theodora Choli ◽  
Manuel Espinosa
Keyword(s):  
Copy Number ◽  
Purification And Characterization ◽  
Copy Number Control

scholarly journals Characterization of the minimal replicon of pHM300 and independent copy number control of major and minor chromosomes ofHaloferax mediterranei

2012 ◽  
Vol 339 (1) ◽  
pp. 66-74 ◽  
Author(s):  
Xiaoqing Liu ◽  
Di Miao ◽  
Fan Zhang ◽  
Zhenfang Wu ◽  
Jingfang Liu ◽  
...  
Keyword(s):  
Copy Number ◽  
Independent Copy ◽  
Copy Number Control

Isolation and characterization of new copy mutants of plasmid R1, and identification of a polypeptide involved in copy number control

1981 ◽  
Vol 181 (1) ◽  
pp. 123-130 ◽  
Author(s):  
Søren Molin ◽  
Peter Stougaard ◽  
Janice Light ◽  
Marie Nordström ◽  
Kurt Nordström
Keyword(s):  
Copy Number ◽  
Copy Number Control ◽  
Isolation And Characterization ◽  
Plasmid R1

scholarly journals Characterization of the Transcriptional Activity of the Cryptic Plasmid pRN1 from Sulfolobus islandicus REN1H1 and Regulation of Its Replication Operon

2006 ◽  
Vol 189 (5) ◽  
pp. 1711-1721 ◽  
Author(s):  
Silvia Berkner ◽  
Georg Lipps
Keyword(s):  
Copy Number ◽  
Transcriptional Activity ◽  
Biochemical Characterization ◽  
Transcriptional Unit ◽  
Plasmid Copy ◽  
Copy Number Control ◽  
Sulfolobus Islandicus ◽  
Control Protein

ABSTRACT The plasmid pRN1 from Sulfolobus islandicus REN1H1 belongs to the crenarchaeal plasmid family pRN. The plasmids in this family encode three conserved proteins that participate in plasmid replication and copy number regulation, as suggested by biochemical characterization of the recombinant proteins. In order to deepen our understanding of the molecular biology of these plasmids, we investigated the transcriptional activity of the model plasmid pRN1. We detected five major transcripts present at about 2 to 15 copies per cell. One long transcriptional unit comprises the genes for the plasmid-copy-number control protein Orf56/CopG and the replication protein Orf904. A second transcript with a long 3′-untranslated region codes for the DNA binding protein Orf80. For both transcripts, we identified countertranscripts which could play a regulatory role. The function of the fifth transcript is unclear. For the five transcripts, we determined the start site, the transcript end, the stability, and the abundance in different growth phases. Reporter gene experiments demonstrated that the copy number control protein Orf56 represses transcription of the orf56-orf904 cotranscript in vivo.

scholarly journals Molecular cloning and functional characterization of a copy number control gene (copB) of plasmid R1.

1982 ◽  
Vol 151 (3) ◽  
pp. 1136-1145 ◽  
Author(s):  
E Riise ◽  
P Stougaard ◽  
B Bindslev ◽  
K Nordström ◽  
S Molin
Keyword(s):  
Molecular Cloning ◽  
Copy Number ◽  
Functional Characterization ◽  
Control Gene ◽  
Copy Number Control ◽  
Plasmid R1
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