The transport of a hepatoprotective agent, isopropryl 2-(l-3-dithiethane-2-ylidene)-2[N(4-methyl-thiazole-2-yl) carbamoyl] acetate (YH439), across Caco-2 cell monolayers

2001 ◽  
Vol 24 (6) ◽  
pp. 584-589 ◽  
Author(s):  
Hyeon Woo Park ◽  
Suk Jae Chung ◽  
Myung Gull Lee ◽  
Chang-Koo Shim
Keyword(s):  
Cell Monolayers ◽  
Hepatoprotective Agent

A pre-embedding, protein a-gold immunolabelling technique for cytocentrifuged cell monolayers for lm and tem

1986 ◽  
Vol 44 ◽  
pp. 220-221
Author(s):  
K. Chien ◽  
I.P. Shintaku ◽  
A.F. Sassoon ◽  
R.L. Van de Velde ◽  
R. Heusser
Keyword(s):  
Cell Surface ◽  
Staining Method ◽  
Protein A ◽  
Surface Antigens ◽  
Cell Suspensions ◽  
Cellular Morphology ◽  
Cellular Phenotype ◽  
Cell Surface Antigens ◽  
Cell Monolayers ◽  
Diagnostic Histopathology

Identification of cellular phenotype by cell surface antigens in conjunction with ultrastructural analysis of cellular morphology can be a useful tool in the study of biologic processes as well as in diagnostic histopathology. In this abstract, we describe a simple pre-embedding, protein A-gold staining method which is designed for cell suspensions combining the handling convenience of slide-mounted cell monolayers and the ability to evaluate specimen staining specificity prior to EM embedding.

Processing Immunoperoxidase Labeled Cell Monolayers and Cryostat Sesctions For Electron Microscopy

1985 ◽  
Vol 43 ◽  
pp. 714-715
Author(s):  
K. Chien ◽  
R. Van de Velde ◽  
I.P. Shintaku ◽  
A.F. Sassoon
Keyword(s):  
Cell Monolayer ◽  
Cell Types ◽  
Surface Antigens ◽  
Immunoperoxidase Method ◽  
Reaction Step ◽  
Hot Plate ◽  
Air Drying ◽  
New Approach ◽  
Cell Monolayers ◽  
Glass Slides

Immunoelectron microscopy of neoplastic lymphoma cells is valuable for precise localization of surface antigens and identification of cell types. We have developed a new approach in which the immunohistochemical staining can be evaluated prior to embedding for EM and desired area subsequently selected for ultrathin sectioning.A freshly prepared lymphoma cell suspension is spun onto polylysine hydrobromide- coated glass slides by cytocentrifugation and immediately fixed without air drying in polylysine paraformaldehyde (PLP) fixative. After rinsing in PBS, slides are stained by a 3-step immunoperoxidase method. Cell monolayer is then fixed in buffered 3% glutaraldehyde prior to DAB reaction. After the DAB reaction step, wet monolayers can be examined under LM for presence of brown reaction product and selected monolayers then processed by routine methods for EM and embedded with the Chien Re-embedding Mold. After the polymerization, the epoxy blocks are easily separated from the glass slides by heatingon a 100°C hot plate for 20 seconds.

Transport in Caco-2 cell monolayers of cucurbitane triterpenoids from Momordica charantia

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
SB Wu ◽  
GGL Yue ◽  
AC Keller ◽  
MH To ◽  
CBS Lau ◽  
...  
Keyword(s):  
Momordica Charantia ◽  
Cell Monolayers

Evaluation of flow-cytometry for the monitoring of infectious human rotavirus in water

1997 ◽  
Vol 35 (11-12) ◽  
pp. 451-453
Author(s):  
F. X. Abad ◽  
A. Bosch ◽  
J. Comas ◽  
D. Villalba ◽  
R. M. Pintó
Keyword(s):  
Flow Cytometry ◽  
Water Samples ◽  
Wild Type ◽  
Cell Monolayers ◽  
Naturally Occurring ◽  
Human Rotavirus ◽  
Faecal Samples

A method has been developed for the detection of infectious human rotavirus (HRV), based on infection of MA104 and CaCo-2 cell monolayers and ulterior flow cytometry. The sensitivity of the flow cytometry procedure for the cell-adapted HRV enabled the detection of 200 and 2 MPNCU in MA104 and CaCo-2 cells, respectively. Flow cytometry performed five days after infection of CaCo-2 enabled the detection of naturally occurring wild-type HRV in faecal samples and concentrated water samples.

Mouse peritoneal cells confer an antiviral state on mouse cell monolayers: role of interferon.

1986 ◽  
Vol 57 (2) ◽  
pp. 456-463 ◽  
Author(s):  
E Proietti ◽  
S Gessani ◽  
F Belardelli ◽  
I Gresser
Keyword(s):  
Mouse Cell ◽  
Cell Monolayers ◽  
Antiviral State ◽  
Peritoneal Cells

scholarly journals Mechanical forces in cell monolayers

2018 ◽  
Vol 131 (24) ◽  
pp. jcs218156 ◽  
Author(s):  
Tianchi Chen ◽  
Thuan Beng Saw ◽  
René-Marc Mège ◽  
Benoit Ladoux
Keyword(s):  
Mechanical Forces ◽  
Cell Monolayers
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