Genotoxicity study of bojungchisup-tang, an oriental herbal decoction-in vitro chromosome aberration assay in Chinese hamster lung cells andin vivo supravital-staining micronucleus assay with mouse peripheral reticulocytes

1998 ◽  
Vol 21 (4) ◽  
pp. 391-397 ◽  
Author(s):  
Jae-Chun Ryu ◽  
Kyung-Ran Kim ◽  
Hyun-Joo Kim ◽  
Ji-Youn Youn ◽  
Seung-Woon Myung ◽  
...  
Keyword(s):  
Chromosome Aberration ◽  
Chinese Hamster ◽  
Micronucleus Assay ◽  
Lung Cells ◽  
Chromosome Aberration Assay ◽  
Herbal Decoction ◽  
Supravital Staining

scholarly journals Toxicological assessment of β-galactosidase shows no adverse effects in vivo and in vitro

2020 ◽  
Vol 4 ◽  
pp. 239784732090877
Author(s):  
Jennifer M Symonds ◽  
Tomohiro Fujita ◽  
Shouhei Aoki ◽  
Kazuma Shiota ◽  
Claire L Kruger
Keyword(s):  
Adverse Effects ◽  
Chromosome Aberration ◽  
Micronucleus Assay ◽  
Sprague Dawley ◽  
Oral Toxicity ◽  
Ames Assay ◽  
Toxicological Assessment ◽  
Chromosome Aberration Assay

A safety assessment for β-galactosidase derived from Aspergillus oryzae (GODO-FAL) was performed. The test article was a concentrated, purified β-galactosidase diluted in glycerin and water with an activity of 10,000 U/mL. A series of genotoxicology tests including micronucleus assay, chromosome aberration assay, and reverse mutagenesis (Ames) assay confirmed that GODO-FAL was not clastogenic or mutagenic at any of the concentrations used, up to 2000 µg/mL for the chromosome aberration assay and 5000 mg per plate in the Ames assay. GODO-FAL was not toxic in acute, repeated oral toxicity, and sub-chronic toxicity assays in Sprague–Dawley rats at any dose used, up to 2000 mg/kg/day. Based on results from the subchronic toxicology assay, the no observed adverse effects level for GODO-FAL was at least 2000 mg/kg/day.

Bamboo leaf extract ameliorates radiation induced genotoxicity: An in vitro study of chromosome aberration assay.

2021 ◽  
pp. 100528
Author(s):  
Shikha Tewari ◽  
Mansi Patel ◽  
Abhipsa VF Debnath ◽  
Priti Mehta ◽  
Snehal Patel ◽  
...  
Keyword(s):  
Chromosome Aberration ◽  
Leaf Extract ◽  
In Vitro Study ◽  
Vitro Study ◽  
Chromosome Aberration Assay ◽  
Radiation Induced

scholarly journals Study on Optimization of Cytoplasm Conditions for In Vitro Micronucleus Test Using Chinese Hamster Lung Cells

2018 ◽  
Vol 37 (3) ◽  
pp. 229-234
Author(s):  
Min Kyoung Paik ◽  
Areumnuri Kim ◽  
Hye Rim Shin ◽  
Kyongmi Chon ◽  
Kyung-Hun Park ◽  
...  
Keyword(s):  
Micronucleus Test ◽  
Chinese Hamster ◽  
Lung Cells

scholarly journals Baseline effects of non-flavored e-liquids in the in vitro micronucleus assay

2019 ◽  
Vol 3 ◽  
pp. 239784731988790 ◽  
Author(s):  
Daniel J Smart ◽  
Fabian R Helbling ◽  
Damian McHugh ◽  
Patrick Vanscheeuwijck
Keyword(s):  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Micronucleus Assay ◽  
Hazard Identification ◽  
Dna Integrity ◽  
Genetic Toxicology ◽  
Data Set ◽  
Toxicological Assessment ◽  
In Vitro Micronucleus Assay

Electronic nicotine delivery systems (ENDSs; e.g. e-cigarettes) are being developed as potentially reduced-risk alternatives to the continued use of conventional tobacco products. They typically comprise a device that heats an e-liquid to generate inhalable vapor. E-liquids and ENDS-derived vapor have been the focus of toxicological assessment; in particular, their DNA-damaging potential has been investigated with varying outcomes and conclusions. In vitro genetic toxicology assays have formed a part of these assessments. However, they are susceptible to producing misleading or false positive results, especially under extreme conditions. In the present study, we evaluated a series of six neat (non-vaporized) non-flavored e-liquids (NFEL-A to F) in a flow cytometry version of the in vitro micronucleus assay in order to characterize their baseline effects on Chinese hamster ovary cells under hazard identification conditions. The NFELs induced cytotoxicity universally despite differing in propylene glycol (PG), vegetable glycerin (VG), and nicotine content. In addition, significant genotoxic responses were also detected with the PG-predominant e-liquids NFEL-A, D, and F but not with NFEL-B, C, or E, which contained higher proportions of VG. All six NFELs induced extreme cell culture conditions (i.e. increases in pH and osmolality) at the concentrations assessed. They also exhibited nonbiologically relevant effects on the mechanistic endpoints (i.e. cell cycle and phosphorylated histones H2AX and H3). In conclusion, although the PG component of the NFELs drove micronucleus formation in the assay, data on the complementary mechanistic endpoints suggest that this apparent DNA damage is potentially misleading and of negligible biological relevance as a risk for DNA integrity. In future assessments, any adverse changes (such as signatures of micronuclei induction, G2M arrest, and increases in γH2AX) relative to this reference data set might indicate a possible genotoxic hazard and would prompt further investigations for exploring the extent of risk.

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