The effect of acteoside on histamine release and arachidonic acid release in RBL-2H3 mast cells

2006 ◽  
Vol 29 (6) ◽  
pp. 508-513 ◽  
Author(s):  
Jin Hee Lee ◽  
Ji Yun Lee ◽  
Hyo Suk Kang ◽  
Chan Hun Jeong ◽  
Hee Moon ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Mast Cells ◽  
Histamine Release ◽  
Arachidonic Acid Release ◽  
Acid Release

scholarly journals A major role for phospholipase A2 in antigen-induced arachidonic acid release in rat mast cells

1987 ◽  
Vol 247 (1) ◽  
pp. 95-99 ◽  
Author(s):  
K Yamada ◽  
Y Okano ◽  
K Miura ◽  
Y Nozawa
Keyword(s):  
Arachidonic Acid ◽  
Mast Cells ◽  
Phospholipase A2 ◽  
Histamine Release ◽  
Phospholipase C ◽  
Minor Role ◽  
Arachidonic Acid Release ◽  
Ige Receptors ◽  
Acid Release ◽  
A Minor

Cross-linking of IgE receptors by antigen stimulation leads to histamine release and arachidonic acid release in rat peritoneal mast cells. Investigators have reported a diverse distribution of [3H]arachidonate that is dependent on labelling conditions. Mast cells from rat peritoneal cavity were labelled with [3H]arachidonic acid for different periods of time at either 30 or 37 degrees C. Optimum labelling was found to be after 4 h incubation with [3H]arachidonate at 30 degrees C, as judged by cell viability (Trypan Blue uptake), responsiveness (histamine release) and distribution of radioactivity. Alterations in 3H-radioactivity distribution in mast cells labelled to equilibrium were examined on stimulation with antigen (2,4-dinitrophenyl-conjugated Ascaris suum extract). The results indicated that [3H]arachidonic acid was lost mainly from phosphatidylcholine and, to a lesser extent, from phosphatidylinositol. A transient appearance of radiolabelled phosphatidic acid and diacylglycerol indicated phosphatidylinositol hydrolysis by phospholipase C. Pretreatment with a phospholipase A2 inhibitor, mepacrine, substantially prevented the antigen-induced liberation of [3H]arachidonic acid from phosphatidylcholine. It can be thus concluded that, in the release of arachidonic acid by antigen-stimulated mast cells, the phospholipase A2 pathway, in which phosphatidylcholine is hydrolysed, serves as the major one, the phospholipase C/diacylglycerol lipase pathway playing only a minor role.

Arachidonic acid release in rat peritoneal mast cells stimulated with antigen, ionophore A23187, and compound 48/80

1985 ◽  
Vol 127 (3) ◽  
pp. 726-732 ◽  
Author(s):  
Yukio Okano ◽  
Yasuhiro Ishizuka ◽  
Shigeru Nakashima ◽  
Toyohiko Tohmatsu ◽  
Hajime Takagi ◽  
...  
Keyword(s):  
Arachidonic Acid ◽  
Mast Cells ◽  
Ionophore A23187 ◽  
Arachidonic Acid Release ◽  
Peritoneal Mast Cells ◽  
Acid Release ◽  
Rat Peritoneal Mast Cells
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