Proteomic analysis and the antimetastatic effect ofN-(4-methyl)phenyl-O-(4-methoxy) phenyl-thionocarbamate-induced apoptosis in human melanoma SK-MEL-28 cells

2006 ◽  
Vol 29 (3) ◽  
pp. 224-234 ◽  
Author(s):  
Su-La Choi ◽  
Yun-Sil Choi ◽  
Young-Kwan Kim ◽  
Nack-Do Sung ◽  
Chang-Won Kho ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Human Melanoma ◽  
Antimetastatic Effect ◽  
Induced Apoptosis ◽  
Methyl Phenyl

Icariside II potentiates paclitaxel-induced apoptosis in human melanoma A375 cells by inhibiting TLR4 signaling pathway

2012 ◽  
Vol 50 (9) ◽  
pp. 3019-3024 ◽  
Author(s):  
Jinfeng Wu ◽  
Ming Guan ◽  
Pok Fai Wong ◽  
Howard Yu ◽  
Jingcheng Dong ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Human Melanoma ◽  
Tlr4 Signaling ◽  
Icariside Ii ◽  
Tlr4 Signaling Pathway ◽  
Induced Apoptosis ◽  
A375 Cells

Caspases Activation in Ultraviolet B-induced Apoptosis of G361 Human Melanoma Cell Line

2000 ◽  
Vol 12 (4) ◽  
pp. 243
Author(s):  
Kyoung Chan Park ◽  
Jeong Gu Lim ◽  
Dong Seok Kim ◽  
Hyun Ok Choi ◽  
Kyu Han Kim ◽  
...  
Keyword(s):  
Cell Line ◽  
Melanoma Cell ◽  
Melanoma Cell Line ◽  
Human Melanoma ◽  
Ultraviolet B ◽  
Human Melanoma Cell Line ◽  
Human Melanoma Cell ◽  
Induced Apoptosis

Suppression of W Damage-Induced Apoptosis of Human Melanoma Cells by a Fermented Milk, Kefir

2005 ◽  
pp. 385-389
Author(s):  
Junko Narisawa ◽  
Tsutomu Nagira ◽  
Ken-ichi Kusumoto ◽  
Kiichiro Teruya ◽  
Yoshinori Katakura ◽  
...  
Keyword(s):  
Fermented Milk ◽  
Melanoma Cells ◽  
Human Melanoma ◽  
Human Melanoma Cells ◽  
Induced Apoptosis

Casticin Induced Apoptosis in A375.S2 Human Melanoma Cells through the Inhibition of NF-κB and Mitochondria-Dependent Pathways In Vitro and Inhibited Human Melanoma Xenografts in a Mouse Model In Vivo

2016 ◽  
Vol 44 (03) ◽  
pp. 637-661 ◽  
Author(s):  
Yin-Wen Shiue ◽  
Chi-Cheng Lu ◽  
Yu-Ping Hsiao ◽  
Ching-Lung Liao ◽  
Jing-Pin Lin ◽  
...  
Keyword(s):  
Human Melanoma ◽  
Oxygen Species ◽  
Protein Levels ◽  
Phase Arrest ◽  
Viable Cells ◽  
M Phase ◽  
Induced Apoptosis ◽  
Western Blotting Assay

Casticin, a polymethoxyflavone occurring in natural plants, has been shown to have anticancer activities. In the present study, we aims to investigate the anti-skin cancer activity of casticin on melanoma cells in vitro and the antitumor effect of casticin on human melanoma xenografts in nu/nu mice in vivo. A flow cytometric assay was performed to detect expression of viable cells, cell cycles, reactive oxygen species production, levels of [Formula: see text] and caspase activity. A Western blotting assay and confocal laser microscope examination were performed to detect expression of protein levels. In the in vitro studies, we found that casticin induced morphological cell changes and DNA condensation and damage, decreased the total viable cells, and induced G2/M phase arrest. Casticin promoted reactive oxygen species (ROS) production, decreased the level of [Formula: see text], and promoted caspase-3 activities in A375.S2 cells. The induced G2/M phase arrest indicated by the Western blotting assay showed that casticin promoted the expression of p53, p21 and CHK-1 proteins and inhibited the protein levels of Cdc25c, CDK-1, Cyclin A and B. The casticin-induced apoptosis indicated that casticin promoted pro-apoptotic proteins but inhibited anti-apoptotic proteins. These findings also were confirmed by the fact that casticin promoted the release of AIF and Endo G from mitochondria to cytosol. An electrophoretic mobility shift assay (EMSA) assay showed that casticin inhibited the NF-[Formula: see text]B binding DNA and that these effects were time-dependent. In the in vivo studies, results from immuno-deficient nu/nu mice bearing the A375.S2 tumor xenograft indicated that casticin significantly suppressed tumor growth based on tumor size and weight decreases. Early G2/M arrest and mitochondria-dependent signaling contributed to the apoptotic A375.S2 cell demise induced by casticin. In in vivo experiments, A375.S2 also efficaciously suppressed tumor volume in a xenotransplantation model. Therefore, casticin might be a potential therapeutic agent for the treatment of skin cancer in the future.

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