Parathyroid hormone like bioactivity measured by cytochemical bioassay in a patient with malignant bladder disease

1988 ◽  
Vol 157 (12) ◽  
pp. 388-389
Author(s):  
S. Roche ◽  
J. F. Fielding ◽  
N. Loveridge
Keyword(s):  
Parathyroid Hormone ◽  
Bladder Disease ◽  
Cytochemical Bioassay

The Cytochemical Bioassay of Parathyroid Hormone: Further Experience

1983 ◽  
Vol 4 (1) ◽  
pp. 1-19 ◽  
Author(s):  
J. Allgrove ◽  
J. Chayen ◽  
J. L. H. O'riordan
Keyword(s):  
Parathyroid Hormone ◽  
Cytochemical Bioassay

Pharmacokinetics of Synthetic Human Parathyroid Hormone 1-34 in man Measured by Cytochemical Bioassay and Radioimmunoassay

1985 ◽  
Vol 68 (2) ◽  
pp. 171-177 ◽  
Author(s):  
G. N. Kent ◽  
N. Loveridge ◽  
J. Reeve ◽  
Joan M. Zanelli
Keyword(s):  
Parathyroid Hormone ◽  
Intravenous Injection ◽  
Time Course ◽  
Biologically Active ◽  
Biological Degradation ◽  
Human Parathyroid Hormone ◽  
Subcutaneous Injections ◽  
Cytochemical Bioassay ◽  
Specific Radioimmunoassay ◽  
Transit Times

1. Synthetic human parathyroid hormone (hPTH) 1-34 was given by intravenous injection to two healthy men. The time course of its appearance in and disappearance from the plasma was monitored both by cytochemical bioassay and by a specific radioimmunoassay (RIA) system. 2. Immunoreactive N-region parathyroid hormone (iPTH) reached peak concentrations in plasma at 2 min after injection, whereas peak concentrations of biologically active parathyroid hormone (bioPTH) were delayed until 4-6 min. Bioassayable PTH-like activity then disappeared from the plasma (mean transit times 5.8 and 8.6 min), approximately twice as fast as immuno-reactivity. 3. After separate subcutaneous administrations, a calculated 22-37% of administered hPTH 1-34 was subsequently detected in the plasma, by both assay systems. 4. It was not possible to explain fully the non-parallel appearances of bio- and immuno-reactivities in the plasma after intravenous injection nor the non-parallel disappearances after both intravenous and subcutaneous injections on the basis of the present data. It seems likely, however, that in the process of biological degradation the immunoreactive locus is inactivated by a different reaction from that which destroys bioactivity. 5. To investigate these activity dissociations further will require the application of microfractionation procedures in conjunction with both types of assay system.

Bioactive parathyroid hormone in pregnant rats and fetuses

1990 ◽  
Vol 258 (4) ◽  
pp. E549-E554 ◽  
Author(s):  
A. Bourdeau ◽  
G. Manganella ◽  
C. L. Thil-Trubert ◽  
C. Sachs ◽  
G. Cournot
Keyword(s):  
Parathyroid Hormone ◽  
Plasma Calcium ◽  
Ether Anesthesia ◽  
Pregnant Rats ◽  
Calcium Gradient ◽  
Osteoclast Number ◽  
Cytochemical Bioassay ◽  
Parathyroid Function ◽  
Immunoreactive Parathyroid Hormone ◽  
Intact Rats

Parathyroid function at the end of gestation (day 21) was investigated by measuring plasma calcium (PCa), immunoreactive parathyroid hormone (iPTH), bioactive parathyroid hormone (bioPTH; cytochemical bioassay), and bone histology in intact and thyroparathyroidectomized (TPTX; day 12, ether anesthesia) rats and their fetuses. In pregnant intact rats, PCa was significantly lower, and iPTH, bioPTH, and osteoclast number were higher than in nonpregnant rats. In fetuses, PCa was higher than maternal PCa and correlated with fetal bioPTH. TPTX suppressed maternal bioPTH and decreased iPTH and osteoclast number, whereas fetal iPTH and bioPTH were decreased with no change in osteoclast number. Fetal PCa was near normal and was correlated with maternal PCa but not with fetal bioPTH. The fetomaternal calcium gradient was maintained and even increased. This study shows that there is maternal physiological hyperparathyroidism and functional fetal parathyroid glands at the end of gestation in the rat. Parathyroid hormone does not seem to be responsible for maintaining the high fetomaternal calcium gradient in TPTX animals.

scholarly journals Interactions between Calciotropic Hormones

1987 ◽  
Vol 80 (8) ◽  
pp. 478-480
Author(s):  
J N Bradbeer
Keyword(s):  
Vitamin D ◽  
Parathyroid Hormone ◽  
Dehydrogenase Activity ◽  
Steroid Hormone ◽  
Vitamin D Metabolites ◽  
Hormone Action ◽  
Calciotropic Hormones ◽  
Cytochemical Bioassay ◽  
Glucose 6 Phosphate Dehydrogenase ◽  
Dose Dependent

The metatarsal cytochemical bioassay (CBA) for parathyroid hormone (PTH) was adapted to study interactions between PTH and certain vitamin D metabolites. Thus, while they had no effect in the system alone, both 1,25(OH)2D3 and 25(OH)D3 caused a dose-dependent potentiation of PTH-stimulated glucose 6-phosphate dehydrogenase activity in the hypertrophic chondrocytes of the rat metatarsal. 1,25(OH)2D3 was about 1000 times more potent than 25(OH)D3. Specificity is indicated by the lack of a similar effect when either oestradiol or 1,24,25(OH)3D3 or a lactone derivative of 1,25(OH)2D3 was used. Furthermore, the rapidity of the effect of 1,25(OH)2D3 and 25(OH)D3, within 8 minutes, favours a membranophilic mechanism rather than the conventional nuclear mechanism of steroid hormone action.

scholarly journals Cytochemical bioassay of parathyroid hormone in maternal and cord blood.

1985 ◽  
Vol 60 (2) ◽  
pp. 110-115 ◽  
Author(s):  
J Allgrove ◽  
S Adami ◽  
R M Manning ◽  
J L O'Riordan
Keyword(s):  
Parathyroid Hormone ◽  
Cord Blood ◽  
Cytochemical Bioassay
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