Use of peroxidase isozyme in testing the genetic purity of some micropropagated plant in sugarcane

Sugar Tech ◽  
2002 ◽  
Vol 4 (3-4) ◽  
pp. 173-175 ◽  
Author(s):  
Ranju Kumari ◽  
V. K. Shahi
Keyword(s):  
Genetic Purity ◽  
Peroxidase Isozyme ◽  
Micropropagated Plant

A modified DNA extraction protocol and its utility in seed genetic purity assessment

2011 ◽  
Vol 39 (1) ◽  
pp. 236-242 ◽  
Author(s):  
S.N. Sharma ◽  
V. Kumar ◽  
G. Singh ◽  
R. Sharma
Keyword(s):  
Dna Extraction ◽  
Genetic Purity ◽  
Purity Assessment ◽  
Extraction Protocol ◽  
Modified Dna

Genetic purity analysis of cotton (Gossypium spp.) hybrids using SSR markers

2010 ◽  
Vol 38 (2) ◽  
pp. 358-366 ◽  
Author(s):  
P. Selvakumar ◽  
R. Ravikesavan ◽  
A. Gopikrishnan ◽  
K. Thiyagu ◽  
S. Preetha ◽  
...  
Keyword(s):  
Ssr Markers ◽  
Genetic Purity ◽  
Gossypium Spp ◽  
Purity Analysis

Molecular cloning and sequences of lignin peroxidase genes of Phanerochaete chrysosporium

1989 ◽  
Vol 9 (6) ◽  
pp. 2743-2747
Author(s):  
H Schalch ◽  
J Gaskell ◽  
T L Smith ◽  
D Cullen
Keyword(s):  
Molecular Cloning ◽  
Phanerochaete Chrysosporium ◽  
Lignin Peroxidase ◽  
Nucleotide Sequences ◽  
Peroxidase Isozyme ◽  
Genomic Clones

The genomic clones encoding lignin peroxidase isozyme H8 and two closely related genes were isolated from Phanerochaete chrysosporium BKM-1767, and their nucleotide sequences were determined. The positions and approximate lengths of introns were found to be highly conserved in all three clones. Analysis of homokaryotic derivatives indicated that the three clones are not alleles of the same gene(s).

scholarly journals Use of molecular marker techniques in seed testing by Brazilian seed companies

1998 ◽  
Vol 55 (spe) ◽  
pp. 79-82 ◽  
Author(s):  
P.T. Della Vecchia ◽  
C.A.R. da Silva ◽  
P. Terenciano-Sobrinho
Keyword(s):  
Molecular Marker ◽  
New Technologies ◽  
Seed Quality ◽  
Genetic Purity ◽  
Process Step ◽  
Seed Testing ◽  
Routine Basis ◽  
Seed Market ◽  
Key Issues ◽  
High Quality Seed

Seed market is becoming global and globalization is growing very fast. To compete favourably in this new global seed world, quality and cost are and will be certanly the key issues. High seed quality can only be obtained by a thorough control of the entire seed production process, step by step from planning to final delivery. That requires science, technology, expertise, experience, good management and certanly, the most important, an absolute and unconditional commitment with quality. Seed testing for quality assurance is one important step in the process of production of high quality seed. In the late years a considerable amount of research has been published, particularly on the use of some Polymerase Chain Reaction DNA based new technologies (RAPD, microsatelites, AFLP) for genetic purity determinations in seed testing. As far as we know, no Brazilian seed company is using, on regular basis, RAPD or other molecular marker techniques in the determination of genetic purity in seed testing. Most of these are using morphological or physiological traits expressed by seed, seedling or mature plant and/or electrophoresis of seed or seedling proteins/isoenzymes for that purpose. Main reasons for that are: DNA molecular marker techniques are relatively new; lack of specialized personnel to run DNA molecular marker assays on routine basis; higher cost/sample when compared to proteins/isoenzymes electrophoresis.

EFFECTS OF ISOLATION DISTANCE ON CONTAMINATION IN SWEETCLOVER

1972 ◽  
Vol 52 (4) ◽  
pp. 517-524 ◽  
Author(s):  
B. P. GOPLEN ◽  
D. A. COOKE ◽  
P. PANKIW
Keyword(s):  
Honey Bees ◽  
Genetic Purity ◽  
Isolation Distance ◽  
Isolation Distances ◽  
Contamination Levels ◽  
High Level

The recessive low coumarin gene cu was used as a marker to study the effects of isolation distance on contamination levels in sweetclover pollinated by honey bees. A 46-m isolation distance was found inadequate to maintain a high level of genetic purity. Considerable contamination from crossing resulted with isolation distances from 46 to 804 m when there was little competitive bloom from other entomophilous crops. A highly attractive and competitive crop of rapeseed appeared to serve as a very effective isolation barrier to minimize contamination. A higher amount of contamination occurred in the borders of the low-coumarin isolation plots of one test. The results of this study are sufficient to question the existing isolation standards for sweetclover, but are not adequate to formulate new standards.

scholarly journals Pengujian Kemurnian Genetik Benih Padi Galur F3 (Pandanwangi x PTB33) Terseleksi Menggunakan Marka Molekuler Simple Sequence Repeats (SSR)

Agrikultura ◽  
2015 ◽  
Vol 26 (2) ◽  
Author(s):  
Syindy Raffini Nasihin ◽  
Wieny H. Rizky ◽  
Nono Carsono
Keyword(s):  
Simple Sequence Repeats ◽  
Simple Sequence Repeat ◽  
Brown Planthopper ◽  
Plant Analysis ◽  
Current Experiment ◽  
Genetic Purity ◽  
Experimental Station ◽  
Purity Testing ◽  
Brown Planthopper Resistance ◽  
Simple Sequence

ABSTRACTSeed Purity Testing of F3 Progeny of Rice Lines Derived from a Cross between Pandanwangi x PTB-33 Estimated by Simple Sequence Repeat MarkersSeeds with high purity is required to produce maximum crop yield. Genetic purity of selected F3rice seed progenies derived from a cross between Pandanwangi x PTB33 was estimated by SSR(simple sequence repeats) molecular markers. Objective of current experiment was to obtain riceseed with high genetic purity (100%) in terms of homogeneity of alleles. The experiment wasconducted at Laboratory of Plant Analysis and Biotechnology, meanwhile field experiment wasperformed at Ciparanje Experimental Station, Faculty of Agriculture, Universitas Padjadjaran.Based on primer designed by Bradbury (aromatic trait) and primers RM589 and RM586 (supposedto correlate with brown planthopper resistance gene), seeds of F3 selected had 100% geneticpurity. SSR markers applied for each offspring were able to demonstrate the purity of the seed.Genotypes with 100% genetic purity can be continued for propagating their seeds.Keywords: F3, seed purity, seed rice, SSR markersABSTRAKBenih dengan kemurnian genetik tinggi sangat diperlukan untuk produksi tanaman yangmaksimal. Kemurnian genetik padi generasi F3 hasil persilangan Pandanwangi x PTB-33 diestimasidengan menggunakan marka molekuler SSR. Percobaan ini bertujuan untuk mendapatkan generasiF3 yang memiliki kemurnian genetik 100%. Penelitian dilaksanakan di Kebun PercobaanCiparanje, Fakultas Pertanian Universitas Padjadjaran dan Laboratorium Analisis dan BioteknologiTanaman. Hasil analisis menggunakan primer Bradbury menunjukkan 100% benih murniberdasarkan karakter aromatik, begitupun berdasarkan karakter ketahanan terhadap wereng(primer RM589 dan RM586) menunjukkan 100% benih murni. Marka molekuler SSR yangdigunakan untuk verifikasi kemurnian mampu menunjukkan kemurnian genetik benih padi yangtinggi. Genotip PP dapat dilanjutkan untuk pengujian dan atau perbanyakan benih sumber.Kata kunci: benih padi, F3, marka SSR, uji kemurnian genetik

scholarly journals Population genetic structure of European wildcats inhabiting the area between the Dinaric Alps and the Scardo-Pindic mountains

2021 ◽  
Author(s):  
Felicita Urzi ◽  
Nikica Šprem ◽  
Hubert Potočnik ◽  
Magda Sindičić ◽  
Dean Konjević ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Population Genetic ◽  
Allelic Richness ◽  
Isolation By Distance ◽  
Domestic Cats ◽  
Genetic Purity ◽  
Eastern Group ◽  
Two Samples ◽  
European Wildcat ◽  
Genetic Clusters

Abstract Habitat fragmentation and loss have contributed significantly to the demographic decline of European wildcat populations and hybridization with domestic cats poses a threat to the loss of genetic purity of the species. In this study we used microsatellite markers to analyse genetic variation and structure of the wildcat populations from the area between the Dinaric Alps and the Scardo-Pindic mountains in Slovenia, Croatia, Serbia and North Macedonia. We also investigated hybridisation between populations of wildcats and domestic cats in the area. One hundred and thirteen samples from free-leaving European wildcats and thirty-two samples from domestic cats were analysed. Allelic richness across populations ranged from 3.61 to 3.98. The observed Ho values ranged between 0.57 and 0.71. The global FST value for the four populations was 0.080 (95% CI 0.056–0.109) and differed significantly from zero (P < 0.001). The highest FST value was observed between the populations North Macedonia and Slovenia and the lowest between Slovenia and Croatia. We also found a signal for the existence of isolation by distance between populations. Our results showed that wildcats are divided in two genetic clusters largely consistent with a geographic division into a genetically diverse northern group (Slovenia, Croatia) and genetically eroded south-eastern group (Serbia, N. Macedonia). Hybridisation rate between wildcats and domestic cats varied between 13% and 52% across the regions.

scholarly journals Identification and characterization of popular rice (Oryza sativa L.) varieties through chemical tests

2020 ◽  
pp. 82-85
Author(s):  
M.S. Nagendra ◽  
P. Selvaraju ◽  
R. Jerlin ◽  
K. Ganesamurthy ◽  
N. Senthil
Keyword(s):  
Tamil Nadu ◽  
Oryza Sativa L ◽  
Colour Change ◽  
Gelatinization Temperature ◽  
Genetic Purity ◽  
Rice Varieties ◽  
Seed Supply ◽  
Crop Varieties ◽  
Identification And Characterization

Identification and characterization of crop varieties are crucial for ensuring the genetic purity of seeds. The present investigation was carried out to identify suitable chemical methods that are fast, reliable and easy for seed analysts, breeders and seed producers for identification of a variety. Twenty-five popular rice varieties in the seed supply chain of Tamil Nadu were subjected to phenol, modified phenol, NaOH, aroma, gelatinization temperature (alkali spreading value), GA3 and 2,4-D tests. The results of the experiment revealed that phenol and modified phenol tests changed the colour of TKM 9 and TRY 1 variety to brown but no colour change was observed in the variety I.W. Ponni variety. The NaOH test is useful for the identification of TKM 9 variety as it changed the colourless solution to red. GA3 and 2,4-D tests characterized the varieties based on the shoot growth into two and three groups respectively. However, all the variety lacked aroma and exhibited a high gelatinization temperature.

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