Parathyroid gland calcium receptor mRNA levels are unaffected by chronic renal insufficiency or low dietary calcium in rats

Endocrine ◽  
1995 ◽  
Vol 3 (11) ◽  
pp. 769-774 ◽  
Author(s):  
Kimberly V. Rogers ◽  
Rebecca L. Conklin ◽  
Stacey H. Lowe ◽  
Barbara A. Petty
Keyword(s):  
Renal Insufficiency ◽  
Parathyroid Gland ◽  
Chronic Renal Insufficiency ◽  
Dietary Calcium ◽  
Mrna Levels ◽  
Calcium Receptor ◽  
Receptor Mrna

Differential regulation of PHEX expression in bone and parathyroid gland by chronic renal insufficiency and 1,25-dihydroxyvitamin D3

2004 ◽  
Vol 286 (4) ◽  
pp. F739-F748 ◽  
Author(s):  
Angela J. Brewer ◽  
Lucie Canaff ◽  
Geoffrey N. Hendy ◽  
Harriet S. Tenenhouse
Keyword(s):  
Renal Insufficiency ◽  
Parathyroid Gland ◽  
Chronic Renal Insufficiency ◽  
Control Diet ◽  
Protein Abundance ◽  
Dihydroxyvitamin D3 ◽  
Dihydroxyvitamin D ◽  
Phex Gene ◽  
Rat Tibia ◽  
Serum Pth

Mutations in the PHEX gene are responsible for X-linked hypophosphatemia, a renal phosphate-wasting disorder associated with defective skeletal mineralization. PHEX is predominantly expressed in bones and teeth and in the parathyroid gland of patients with chronic renal failure and tertiary hyperparathyroidism. The purpose of the present study was to examine the effects of renal insufficiency and 1,25-dihydroxyvitamin D3 [1,25(OH)2D3] on the regulation of PHEX expression in rat tibia and parathyroid gland. In rats fed a high-phosphate (Pi) diet, ⅚ nephrectomy elicited a significant increase in the serum parathyroid hormone (PTH) concentration that was associated with a significant increase in the abundance of PHEX mRNA and protein in the tibia and a significant increase in PHEX mRNA in the parathyroid gland. In contrast, 1,25(OH)2D3 administration to intact rats fed a control diet elicited a significant decrease in the serum PTH concentration that was accompanied by a significant decrease in PHEX mRNA and protein abundance in the tibia and a significant decrease in PHEX mRNA in the parathyroid gland. In addition, the increases in serum PTH levels and PHEX mRNA in the tibia and parathyroid gland in ⅚ nephrectomized rats fed a high-Pi diet were blunted by 1,25(OH)2D3. Serum PTH concentration was positively and significantly correlated with tibial PHEX mRNA and protein abundance. In summary, we demonstrate that PHEX expression in the tibia and parathyroid gland is increased by chronic renal insufficiency and decreased by 1,25(OH)2D3 administration and suggest that PTH status may play an important role in mediating these changes in PHEX expression.

scholarly journals Unfavorable Reduction in the Ratio of Endothelin B to A Receptors in Experimental 5/6 Nephrectomy and Adenine Models of Chronic Renal Insufficiency

2020 ◽  
Vol 21 (3) ◽  
pp. 936
Author(s):  
Suvi Törmänen ◽  
Päivi Lakkisto ◽  
Arttu Eräranta ◽  
Peeter Kööbi ◽  
Ilkka Tikkanen ◽  
...  
Keyword(s):  
Renal Insufficiency ◽  
Chronic Renal Insufficiency ◽  
Rat Kidney ◽  
Kidney Cortex ◽  
Mrna Levels ◽  
Endothelin Receptor ◽  
Protein Ratio ◽  
Protein Levels ◽  
Mrna Ratio ◽  
Endothelin B

Chronic renal insufficiency (CRI) is characterized by increased endothelin 1 (ET-1) synthesis. We studied rat kidney endothelin receptor A (ETA) and receptor B (ETB) expressions after 12 and 27 weeks of 5/6 nephrectomy, and after 12 weeks of 0.3% adenine diet, representing proteinuric and interstitial inflammation models of CRI, respectively. Uric acid and calcium-phosphate metabolism were modulated after 5/6 nephrectomy, while ETA blocker and calcimimetic were given with adenine. Endothelin receptor mRNA levels were measured using RT-qPCR and protein levels using autoradiography (5/6 nephrectomy) or ELISA (adenine model). Both 12 and 27 weeks after 5/6 nephrectomy, kidney cortex ETA protein was increased by ~60% without changes in ETB protein, and the ETB:ETA ratio was reduced. However, the ETB:ETA mRNA ratio did not change. In the adenine model, kidney ETA protein was reduced by ~70%, while ETB protein was suppressed by ~95%, and the ETB:ETA ratio was reduced by ~85%, both at the protein and mRNA levels. The additional interventions did not influence the observed reductions in the ETB:ETA ratio. To conclude, unfavorable reduction in the ETB:ETA protein ratio was observed in two different models of CRI. Therefore, ETA blockade may be beneficial in a range of diseases that cause impaired kidney function.

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