Surface plasmon resonance imaging analysis of hexahistidine-tagged protein on the gold thin film coated with a calix crown derivative

2004 ◽  
Vol 9 (2) ◽  
pp. 143-146 ◽  
Author(s):  
Seung-Hak Baek ◽  
Yong-Beom Shin ◽  
Min-Gon Kim ◽  
Hyeon-Su Ro ◽  
Eun-Ki Kim ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Surface Plasmon Resonance Imaging ◽  
Imaging Analysis ◽  
Resonance Imaging ◽  
Gold Thin Film

Surface Plasmon Resonance Imaging ( SPRi ): An Inexpensive Tool to Study the Water Transport in Thin Film PFSA Ionomers

2019 ◽  
Author(s):  
Shahab Bayani Ahangar ◽  
Kishan Bellur ◽  
Ezequiel Medici ◽  
Kazuya Tajiri ◽  
Jeffrey S. Allen ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Water Transport ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Imaging Techniques ◽  
Low Cost ◽  
Proton Exchange ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging

The kinetics of water transport in confined thin film Perfluorinated sulfonic-acid (PFSA) ionomers is of vital importance in various applications such as a proton-exchange membrane or catalyst layers in polymer-electrolyte fuel cells. Advanced imaging techniques such as Neutron reflectivity, grazing-incidence x-ray scattering, and atomic force microscopy have been used for studying interfacial water transport in thin-film ionomers. The instruments mentioned are considered high-end, expensive, super-resolution microscopes. The need for an expensive microscopic apparatus restricts many laboratories in developing countries from conducting experiments in the field of interfacial sciences such as visualization and in-situ measurement of water transport in thin-film PFSA ionomers due to financial constraints, limited infrastructure, and lack of high-end technical support. Following the notion of portable and low-cost technologies, which is a vision of many researchers, we introduced the application of surface plasmon resonance imaging (SPRi) in the visualization of diffusion transport phenomena of water in thin-film ionomers. In this work, the smartphone-based Surface plasmon resonance imaging with 3D printed optical components that cost less than $350 is proposed for imaging of water transport in thin-film ionomer.

scholarly journals Development of automated angle-scanning, high-speed surface plasmon resonance imaging and SPRi visualization for the study of dropwise condensation

2019 ◽  
Vol 61 (1) ◽  
Author(s):  
Shahab Bayani Ahangar ◽  
Vinaykumar Konduru ◽  
Jeffrey S. Allen ◽  
Nenad Miljkovic ◽  
Seong Hyuk Lee ◽  
...  
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Speed ◽  
Dropwise Condensation ◽  
Surface Plasmon Resonance Imaging ◽  
Resonance Imaging ◽  
Film Rupture ◽  
Image Artifact

Abstract This paper describes the fabrication and testing of a novel angle-scanning surface plasmon resonance imaging (SPRi) instrument. The combination of two stationary mirrors and two angle-controlled mirrors provides high accuracy (up to 10−3°) and high-speed angular probing. This instrument minimizes the angle-dependent image artifact that arises due to beam walk, which is the biggest challenge for the use of SPRi with angular modulation (AM). In the work described in this paper, two linear stages were employed to minimize the image artifact by adjusting the location of the angle-controlled mirrors and the camera. The SPRi instrument was used to visualize coalescence during dropwise condensation. The results show that the effect of the environment’s temperature on reflectance was less than 1% when the incident angle was carefully chosen for SPRi with intensity modulation (IM). This means that condensation visualization can be carried out at ambient temperatures, without the need for a Peltier stage or a thermally controlled condensing surface. The concept of pixel neighboring was employed to assess the probability of noise and the standard error of thin film measurement. Experimental analyses during dropwise condensation show (1) the presence of a thin film with thickness of one monolayer, and (2) surface coverage of 0.71 m2/m2 by the thin film in the area between the droplets. In addition, analyses showed the existence of a dry area at the part of the substrate exposed by coalescence to ambient air. The results of this work undermine the validity of the film rupture theory as the dropwise condensation mechanism. Graphic abstract

Surface Plasmon Resonance Imaging: A Technique to Reveal the Dropwise Condensation Mechanism

2020 ◽  
Vol 142 (3) ◽  
Author(s):  
Shahab Bayani Ahangar ◽  
Jeffrey S. Allen ◽  
Seong Hyuk Lee ◽  
Chang Kyoung Choi
Keyword(s):  
Thin Film ◽  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
High Temporal Resolution ◽  
Dropwise Condensation ◽  
Surface Plasmon Resonance Imaging ◽  
Label Free ◽  
Resonance Imaging ◽  
Ambient Conditions

Abstract To understand the physics behind dropwise condensation, a microscopy technique must be able to measure a sub-nanometer film at a high temporal resolution (>1,000 FPS). In this work, automated Surface Plasmon Resonance imaging (SPRi) is used as a tool to study the existence of a thin film between the dropwise condensate. SPRi is a label-free imaging technique that works based on the attenuated total internal reflection. SPRi can detect changes in the refractive index (RI) of the test medium in the thin region (<300 nm) above the sensing gold layer. The automated, angle-scanning SPRi instrument was developed by integrating linear and rotating motorized stages. This instrument improves conventional SPRi by enhancing the resolution of angle probing, increasing the speed of angle scanning, and minimizing the angle-dependent image artifacts. As a proof of concept, we visualized the three stages of coalescence at 10,000 FPS, including bridge formation, composite peanut-shape droplet formation, and the relaxation stage. Furthermore, we probed the solid-vapor interface during the dropwise condensation to evaluate the existence of a thin film on the substrate. The results of our visualization show that the area between droplets is covered by an adsorbed film with a thickness of a monolayer (0.275 nm) and a surface coverage of less than one (m2/m2). Moreover, the results reveal a dry region forms on the substrate when part of the substrate is exposed to ambient conditions due to the coalescence. The dry zone on the substrate has higher surface energy, as compared to the surrounding area. Therefore, the exposed area serves as a favorable site for vapor molecules to strike the surface and form new nuclei.

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