A bio-fluidic device for adaptive sample pretreatment and its application to measurements ofEscherichia coli concentrations

2006 ◽  
Vol 11 (1) ◽  
pp. 54-60 ◽  
Author(s):  
Wonjae Choi ◽  
Je-Kyun Park
Keyword(s):  
Sample Pretreatment ◽  
Ofescherichia Coli ◽  
Fluidic Device

Morphology and growth pattern of a rod-negativeenv B mutant ofEscherichia coli K12

1974 ◽  
Vol 14 (6) ◽  
pp. 465-477 ◽  
Author(s):  
G. D. Bloom ◽  
J. Gumpert ◽  
S. Normark ◽  
E. Schuhmann ◽  
U. Taubeneck ◽  
...  
Keyword(s):  
Growth Pattern ◽  
Ofescherichia Coli

Growth and cell division ofEscherichia coli 173-25 in the presence of some analogues of diaminopimelic acid

1974 ◽  
Vol 14 (4) ◽  
pp. 283-296 ◽  
Author(s):  
J. Chaloupka ◽  
M. Strnadová ◽  
J. Čáslavská ◽  
K. Vereš
Keyword(s):  
Cell Division ◽  
Ofescherichia Coli ◽  
Diaminopimelic Acid

Fatty acid composition of lipids ofEscherichia coli W 1655 F+ and its stable protoplast type L-form

1982 ◽  
Vol 22 (3) ◽  
pp. 169-174 ◽  
Author(s):  
J. Gumpert ◽  
W. Schade ◽  
D. Krebs ◽  
S. Baykousheva ◽  
E. Ivanova ◽  
...  
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Acid Composition ◽  
Ofescherichia Coli

scholarly journals Urine lipoarabinomannan in HIV uninfected, smear negative, symptomatic TB patients: effective sample pretreatment for a sensitive immunoassay and mass spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Anita G. Amin ◽  
Prithwiraj De ◽  
Barbara Graham ◽  
Roger I. Calderon ◽  
Molly F. Franke ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Sample Pretreatment ◽  
Positive Association ◽  
Proteinase K ◽  
Clinical Samples ◽  
Sputum Smear ◽  
Smear Negative ◽  
Tuberculostearic Acid ◽  
Negative Sputum ◽  
Adult Culture

AbstractOur study sought to determine whether urine lipoarabinomannan (LAM) could be validated in a sample cohort that consisted mainly of HIV uninfected individuals that presented with tuberculosis symptoms. We evaluated two tests developed in our laboratory, and used them on clinical samples from Lima, Peru where incidence of HIV is low. ELISA analysis was performed on 160 samples (from 140 adult culture-confirmed TB cases and 20 symptomatic TB-negative child controls) using 100 μL of urine after pretreatment with Proteinase K. Two different mouse monoclonal antibodies-CS35 and CHCS9-08 were used individually for capture of urine LAM. Among cases, optical density (OD450) values had a positive association with higher bacillary loads. The 20 controls had negative values (below the limit of detection). The assay correctly identified all samples (97–100% accuracy confidence interval). For an alternate validation of the ELISA results, we analyzed all 160 urine samples using an antibody independent chemoanalytical approach. Samples were called positive only when LAM surrogates—tuberculostearic acid (TBSA) and d-arabinose (d-ara)—were found to be present in similar amounts. All TB cases, including the 40 with a negative sputum smear had LAM in detectable quantities in urine. None of the controls had detectable amounts of LAM. Our study shows that urinary LAM detection is feasible in HIV uninfected, smear negative TB patients.

scholarly journals ATPase activity of the MsbA lipid flippase ofEscherichia coli.

2002 ◽  
Vol 277 (46) ◽  
pp. 44588
Author(s):  
William T. Doerrler ◽  
Christian R.H. Raetz
Keyword(s):  
Atpase Activity ◽  
Ofescherichia Coli ◽  
Lipid Flippase
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