On-line monitoring of IPTG induction for recombinant protein production using an automatic pH control signal

2005 ◽  
Vol 10 (4) ◽  
pp. 304-308 ◽  
Author(s):  
Won Hur ◽  
Yoon-Keun Chung
Keyword(s):  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Ph Control ◽  
Control Signal ◽  
Iptg Induction ◽  
On Line

scholarly journals Optogenetic lac operon to control chemical and protein production in Escherichia coli with light

2019 ◽  
Author(s):  
Makoto A. Lalwani ◽  
Samantha S. Ip ◽  
Cesar Carrasco-Lopez ◽  
Evan M. Zhao ◽  
Hinako Kawabe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Engineering ◽  
Recombinant Protein ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Lac Operon ◽  
Iptg Induction ◽  
Spatial Control ◽  
Chemical Inducers ◽  
Cell Functions

AbstractControl of the lac operon with IPTG has been used for decades to regulate gene expression in E. coli for countless applications, including metabolic engineering and recombinant protein production. However, optogenetics offers unique capabilities such as easy tunability, reversibility, dynamic induction strength, and spatial control that are difficult to obtain with chemical inducers. We developed an optogenetic lac operon in a series of circuits we call OptoLAC. With these circuits, we control gene expression from various IPTG-inducible promoters using only blue light. Applying them to metabolic engineering improves mevalonate and isobutanol production by 24% and 27% respectively, compared to IPTG induction, in light-controlled fermentations scalable to at least 2L bioreactors. Furthermore, OptoLAC circuits enable light control of recombinant protein production, reaching yields comparable to IPTG induction, but with enhanced tunability of expression and spatial control. OptoLAC circuits are potentially useful to confer light controls over other cell functions originally engineered to be IPTG-inducible.

Continuous bleed recycling significantly increases recombinant protein production yield in perfusion cell cultures

2021 ◽  
Vol 169 ◽  
pp. 107966
Author(s):  
Jean-Marc Bielser ◽  
Mathieu Aeby ◽  
Stefania Caso ◽  
Anaïs Roulet ◽  
Hervé Broly ◽  
...  
Keyword(s):  
Recombinant Protein ◽  
Cell Cultures ◽  
Recombinant Protein Production ◽  
Protein Production ◽  
Production Yield
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