Effectiveness ofCyanothece spp. andCyanospira capsulata exocellular polysaccharides as antiadhesive agents for blocking attachment ofHelicobacter pylori to human gastric cells

2004 ◽  
Vol 49 (1) ◽  
pp. 64-70 ◽  
Author(s):  
F. Ascencio ◽  
N. L. Gama ◽  
R. De Philippis ◽  
B. Ho
Keyword(s):  
Gastric Cells ◽  
Exocellular Polysaccharides

Synaptic regulation of cellular properties and burst oscillations of neurons in gastric mill system of spiny lobsters, Panulirus interruptus

1984 ◽  
Vol 52 (1) ◽  
pp. 54-73 ◽  
Author(s):  
D. F. Russell ◽  
D. K. Hartline
Keyword(s):  
Motor Neurons ◽  
Pattern Generator ◽  
Stomatogastric Ganglion ◽  
Gastric Mill ◽  
Panulirus Interruptus ◽  
Synaptic Regulation ◽  
Gastric Cells ◽  
Current Pulses ◽  
Regenerative Property ◽  
Stimulation Of

The properties of neurons in the stomatogastric ganglion (STG) participating in the pattern generator for the gastric mill rhythm were studied by intracellular current injection under several conditions: during ongoing gastric rhythms, in the nonrhythmic isolated STG, after stimulation of the nerve carrying central nervous system (CNS) inputs to the STG, or under Ba2+ or Sr2+. Slow regenerative depolarizations during ongoing rhythms were demonstrated in the anterior median, cardiopyloric, lateral cardiac, gastropyloric, and continuous inhibitor (AM, CP, LC, GP, and CI) neurons according to criteria such as voltage dependency, burst triggering, and termination by brief current pulses, etc. Experiments showed that regenerative-like behavior was not due to synaptic network interactions. The slow regenerative responses were abolished by isolating the stomatogastric ganglion but could be reestablished by stimulating the input nerve. This indicates that certain CNS inputs synaptically induce the regenerative property in specific gastric neurons. Slow regenerative depolarizations were not demonstrable in gastric mill (GM) motor neurons. Their burst oscillations and firing rate were instead proportional to injected current. CNS inputs evoked a prolonged depolarization in GM motor neurons, apparently by a nonregenerative mechanism. All the gastric cells showed prolonged regenerative potentials under 0.5-1.5 mM Ba2+. We conclude that the gastric neurons of the STG can be divided into three types according to their properties: those with a regenerative capability, a repetitively firing type, and a nonregenerative "proportional" type. The cells are strongly influenced by several types of CNS inputs, including "gastric command fibers."

scholarly journals Helicobacter pylori and the BMP pathway regulate CDX2 and SOX2 expression in gastric cells

2012 ◽  
Vol 33 (10) ◽  
pp. 1985-1992 ◽  
Author(s):  
V. Camilo ◽  
R. Barros ◽  
S. Sousa ◽  
A. M. Magalhaes ◽  
T. Lopes ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Sox2 Expression ◽  
Gastric Cells ◽  
Bmp Pathway

scholarly journals Helicobacter pylori Infection Induces Genetic Instability of Nuclear and Mitochondrial DNA in Gastric Cells

2009 ◽  
Vol 15 (9) ◽  
pp. 2995-3002 ◽  
Author(s):  
Ana Manuel Dantas Machado ◽  
Ceu Figueiredo ◽  
Eliette Touati ◽  
Valdemar Máximo ◽  
Sonia Sousa ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Mitochondrial Dna ◽  
Genetic Instability ◽  
Helicobacter Pylori Infection ◽  
Gastric Cells

scholarly journals Afadin Downregulation by Helicobacter pylori Induces Epithelial to Mesenchymal Transition in Gastric Cells

2018 ◽  
Vol 9 ◽  
Author(s):  
Miguel Sardinha Marques ◽  
Joana Melo ◽  
Bruno Cavadas ◽  
Nuno Mendes ◽  
Luísa Pereira ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Epithelial To Mesenchymal Transition ◽  
Mesenchymal Transition ◽  
Gastric Cells

scholarly journals microRNA-29a-3p, Up-Regulated in Human Gastric Cells and Tissues with H.Pylori Infection, Promotes the Migration of GES-1 Cells via A20-Mediated EMT Pathway

2018 ◽  
Vol 51 (3) ◽  
pp. 1250-1263 ◽  
Author(s):  
Fengying Sun ◽  
Ying Ni ◽  
Hong Zhu ◽  
Jian Fang ◽  
Hua Wang ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Epithelial Cells ◽  
Western Blotting ◽  
Molecular Mechanisms ◽  
Luciferase Reporter ◽  
Human Gastric Mucosa ◽  
Gastric Epithelial Cells ◽  
Migration Ability ◽  
Gastric Cells ◽  
Dose Dependent

Background/Aims: Helicobacter pylori (H. pylori) infection is closely related to human gastric mucosa-associated diseases. Several recent studies on miRNAs have expanded our insights on H.pylori pathogenesis. This study aimed to investigate the biological roles and underlying molecular mechanisms of miR-29a-3p in human gastric cells and tissues with H.pylori infection. Methods: miR-29a-3p expression was quantified by quantitative RT-PCR (qRT-PCR). A miR-29a-3p target gene was validated by bioinformatics analysis, western blotting and dual luciferase reporter gene assays. Western blotting and immunohistochemistry (IHC) assay were performed to detect the protein expression. Transwell assay was used to determine the cell migration ability. Results: MiR-29a-3p was up-regulated in H.pylori-positive gastric mucosa tissues and H.pylori-infected gastric cells. The up-regulation of miR-29a-3p was dose-dependent in BGC-823 and GES-1 cells infected with H.pylori. Using gain- and loss-of-function experiments in vitro, we demonstrated that miR-29a-3p promoted the migration of gastric epithelial cells. We further characterized A20 as a direct target of miR-29a-3p. The expression of A20 was decreased in H.pylori-positive gastric mucosa tissues compared with H.pylori-negative gastric mucosa tissues. A20 downregulation was time- and dose-dependent in GES-1 and BGC-823 cells infected with H.pylori. In GES-1 and BGC-823 cells infected with H.pylori, the miR-29a-3p mimic significantly blocked A20 expression, which suggests that H.pylori decreased A20 expression through up-regulating miR-29a-3p in GES-1 and BGC-823 cells infected with H.pylori. The knockdown of A20 by siRNA enhanced the migration of human gastric epithelial cells and promoted the expression of Snail, Vimentin, and N-cadherin and inhibited the expression of E-cadherin. Conclusion: The miR-29a-3p may act as a tumor promotive miRNA by regulating cells migration through directly targeting of A20 gene in human gastric epithelial cells infected with H.pylori.

scholarly journals Performances of different protocols for exocellular polysaccharides extraction from milk acid gels: Application to yogurt

2018 ◽  
Vol 239 ◽  
pp. 742-750 ◽  
Author(s):  
An Thi-Binh Nguyen ◽  
Michaël Nigen ◽  
Luciana Jimenez ◽  
Hassina Ait-Abderrahim ◽  
Sylvie Marchesseau ◽  
...  
Keyword(s):  
Exocellular Polysaccharides

Co-lyophilized Aspirin with Trehalose Causes Less Injury to Human Gastric Cells and Gastric Mucosa of Rats

2016 ◽  
Vol 61 (8) ◽  
pp. 2242-2251 ◽  
Author(s):  
Lee-Shuan Lin ◽  
Yuko Kayasuga-Kariya ◽  
Shugo Nakamura ◽  
Nobuyuki Shimohata ◽  
Takamasa Sakai ◽  
...  
Keyword(s):  
Gastric Mucosa ◽  
Gastric Cells
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